Clinical application and drug-use-guidance value of metagenomic next-generation sequencing in central nervous system infection.

Objective: Timely and precise etiology diagnosis is crucial for optimized medication regimens and better prognosis in central nervous system infections (CNS infections). We aimed to analyze the impact of mNGS tests on the management of patients with CNS infections.

Methods: We conducted a single-center retrospective cohort study to analyze the value of mNGS in clinical applications.

The direct application of plasma droplet digital PCR in the ultra-early pathogen detection and warning during sepsis: Case reports.

Background: Sepsis is a severe and acute disease associated with high mortality, for those who survive, long-term morbidity. In-time detection of pathogen for proper and optimized treatment in the early stage of sepsis is crucial.

Methods: We performed droplet digital PCR (ddPCR) on two cases of sepsis.