Publications by authors named "Yuan-Hai You"
Background: () persistently colonizes the human gastric mucosa in more than 50% of the global population, leading to various gastroduodenal diseases ranging from chronic gastritis to gastric carcinoma. Cytotoxin-associated gene A (CagA) protein, an important oncoprotein, has highly polymorphic Glu-Pro-Ile-Tyr-Ala segments at the carboxyl terminus, which play crucial roles in pathogenesis. Our previous study revealed a significant association between amino acid deletions at positions 893 and 894 and gastric cancer.
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- - The study examined the distribution of the (E) resistance gene in 96 bacterial isolates from a Beijing hospital, determining that 47.92% of them were positive for this gene, primarily belonging to a specific strain type (ST78).
- - Through mating tests, the (E) gene was successfully transferred between bacterial strains, demonstrating notable transferability and resulting in a transconjugant with significantly higher quinupristin-dalfopristin resistance compared to the original strains.
- - Genome sequencing revealed that the plasmid carrying the (E) gene shares high similarity with existing plasmids and contains multiple antibiotic resistance genes, indicating its potential role in the spread of multidrug resistance in bacterial populations.
This study aimed to determine the in vitro activity of quinupristin-alfopristin against Streptococcus sp. isolated in China. This agent is not yet available for clinical use, but it has been tested against a high proportion of resistant Staphylococcus aureus strains.
View Article and Find Full Text PDFObjective: To investigate molecular characterization of streptococcus pyogenes isolates involved in an outbreak of scarlet fever in China in 2011.
Methods: Seventy-four Streptococcal pyogenes involved in an outbreak of scarlet fever were isolated from pediatric patients in the areas with high incidence in China from May to August of 2011. Emm genotyping, pulsed-field gel electrophoresis (PFGE), superantigen (SAg) genes and antimicrobial susceptibility profiling were analyzed for these isolates.
Objective: To investigate the protein expression profiles of the major food-borne pathogen Campylobacter jejuni NCTC11168.
Methods: Membrane and soluble cellular proteins were extracted from the genome-sequenced C. jejuni strain NCTC11168.
Objective: To investigate genomic variations of two Chinese Yersinia pestis isolates that were isolated from different plague foci obtained from vaccine strain EV76 from the Yunnan province of China.
Methods: A microarray containing 12 000 probes covering the entire genome of seven Yersinia pestis and two Yersinia pseudotuberculosis strains, was used. PCR assays were performed to confirm microarray results.
Objective: The quality of microarray data influences the accuracy of comparative genomic analyses to a large extent. To ensure that the results obtained by using an in situ synthesized microarray are accurate, data quality is to be assessed by evaluating the melting temperature (Tm) of probes, probability of false synthesis rates, and fragmentation of labeled targets.
Methods: DNA from the Yersinia pestis vaccine strain EV76 was used for microarray analyses.
Time-series gene expression profiles in AGS cells stimulated with Helicobacter pylori.
World J Gastroenterol
March 2010
Article Synopsis
- The study aimed to deepen understanding of how Helicobacter pylori interacts dynamically with the stomach lining.
- Researchers used cDNA microarrays to analyze gene expression in gastric cancer cells infected with H. pylori over six time points.
- Results revealed varying gene expression patterns linked to immune and tumor-related pathways, suggesting early infection could influence infection outcomes.*
Article Synopsis
- A new technique was developed to simultaneously detect multiple target genes in Roundup Ready soybean using a combination of multiplex PCR and low-density DNA microarray.
- The method involved designing specific probes and primers for various genetically modified crops, emphasizing goal to avoid false results through control probes.
- The resulting technique demonstrated high specificity and sensitivity, successfully identifying GM crops at very low concentrations (0.5% transgenic events).
[In-vitro activity of rabeprazole, lansoprazole, and esomeprazole against Helicobacter pylori].
Zhonghua Liu Xing Bing Xue Za Zhi
June 2003
Objective: To investigate the antimicrobial activity of Pariet, Tekpron, Nexium, respectively, against Helicobacter pylori (H. pylori) in vitro.
Methods: Antimicrobial effects of these medicines were evaluated through detection of MICs for 3 H.