Field evaluation of two commercial RT-rtPCR assays for porcine reproductive and respiratory syndrome virus detection using sera from ill and healthy pigs, China.

Porcine reproductive and respiratory syndrome virus (PRRSV) is a highly contagious respiratory virus causing severe morbidity in pigs worldwide. Control strategies for PRRSV often rely on detecting PRRSV, culling or isolating sick pigs, disinfecting pig barns, vaccination, and monitoring for virus spread. Given the high economic impact of PRRSV on pig farms, there is a great need for rapid and reliable PRRSV detection assays.

Clinical investigation for bilateral cleft lip repair: modified functional bilateral cleft lip cheilorrhaphy.

Purpose: To obtain better operative results, a modified functional bilateral cleft lip (BCL) cheilorrhaphy was designed and used on 131 BCL patients to evaluate the clinical effectiveness in this clinical investigation. A new surgical method would be provided for BCL patients.

Patients And Methods: Based on the experiences and advantages of the commonly used self-longation and elongation surgical methods for BCL repair, a new surgical method was designed for BCL patients.

[HBsAg uptake into human trophoblasts cultured in vitro].

Objective: To detect the entry of HBsAg into human trophoblasts cultured in vitro, to provide some clues for the mechanism responsible for HBV intrauterine transmission.

Methods: Digestion with both trypsin (0.25%) and Dnase I(0.