One-Pot Assay Based on CRISPR/Cas13a Technology for HEV RNA Point-of-Care Testing.

Hepatitis E virus (HEV) poses a serious threat to both public health and animal food safety, thereby highlighting the demands for rapid, sensitive, and easy-to-use detection. This study aimed to develop a One-Pot assay using CRISPR/Cas13a for detecting HEV RNA, suitable for point-of-care testing (POCT) in resource-limited settings. CRISPR/Cas13a combined with reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription recombinase-aided amplification (RT-RAA) was applied to a One-Pot assay device.

Depressed TFAM promotes acetaminophen-induced hepatotoxicity regulated by DDX3X-PGC1α-NRF2 signaling pathway.

Background: Acetaminophen (APAP)-induced acute liver injury (AILI) is the most prevalent cause of acute liver failure and mitochondrial dysfunction plays a dominant role in the pathogenesis of AILI. Mitochondrial transcription factor A (TFAM) is an important marker for maintaining mitochondrial functional homeostasis, but its functions in AILI are unclear. This study aimed to investigate the function of TFAM and its regulatory molecular mechanism in the progression of AILI.

The efficacy of bioretention systems amended with iron-modified biochar for the source-separated and component-specific treatment of rainwater runoff: A microbiome perspective.

Bioretention systems offer advantages in controlling non-point source pollution from runoff rainwater. However, the systems frequently encounter challenges, including insufficient stability of nitrogen and phosphorus removal. Limited research has been performed on bioretention systems which integrate actual data from non-point source pollution cases for the quantitative and qualitative refinement of initial and non-initial rainwater.

[Construction and validation of a prediction model for prolonged hospitalization in patients with severe acute pancreatitis].

Objective: To construction the risk factors associated with prolonged hospitalization in patients with severe acute pancreatitis (SAP) and develop a prediction model for assessing these risks.

Methods: SAP patients admitted to the department of emergency of Hebei Province Cangzhou Hospital of Integrated Traditional Chinese and Western Medicine from January 2015 to December 2023 were retrospectively selected as the study subjects. The 75% of hospital stay was used as the cut-off point, and the patients were categorized into a normal group and an extended group.

Near-infrared fluorescence imaging tool with large Stokes shift for sensitively detecting carboxylesterase 2 and monitoring its expression in non-alcoholic fatty liver disease.

Non-alcoholic fatty liver disease (NAFLD) now affects more than one quarter of the global population and becomes a heavy public health burden. However, the underlying mechanism for the pathogenesis of NAFLD is still not clear. Carboxylesterase 2 (CES2), highly abundant in the liver and intestine, plays an important role in endogenous lipid metabolism and lipolysis.

BMAL1 upregulates STX17 levels to promote autophagosome-lysosome fusion in hippocampal neurons to ameliorate Alzheimer's disease.

We aim to investigate muscle ARNT-like protein 1 (BMAL1) regulation of syntaxin17 (STX17) in mouse hippocampal neurons, focusing on autophagy and amyloid-β (Aβ) deposition. Autophagosome-lysosome fusion in APP/PS1 hippocampal tissues was observed using transmission electron microscopy, while mRNA levels of LC3II and P62 were measured via reverse-transcription PCR (RT-PCR) after Amyloid precursor protein (APP) overexpression. STX17, linked to autophagy and differentially expressed in Alzheimer's disease (AD) brains, was knocked down or overexpressed to assess its effects.

Glycerol Modulated Collagen Fibril Evolution and Lamellar Organization for Biomimetic Corneal Substitutes Construction.

Collagen as the main structural component of the cornea exhibits unique and highly organized fibril lamellae, which contribute to the maintenance of corneal structure and transparency. Nevertheless, collagen assembly in vitro to create ideal artificial corneal substitutes with human cornea comparable thickness and optics is still limited. Here, glycerol as a regulator can reconcile collagen thickness, transparency, and permeability, a conflicting goal by current keratoprosthesis strategies.

Advantages of using touch-controlled, minimally invasive implantation technique on soft tissue in the aesthetic zone of maxillary anterior teeth.

Endosseous implant is an ideal treatment option for the treatment of denture defects and omissions. However, how to maintain the perfect gum contour of patients has been an important research topic for stomatologists and has attracted much attention. The objective of this study was to explore the advantages of minimally invasive touch-controlled implantation in maintaining soft tissue morphology in the aesthetic area of maxillary anterior teeth.

Study on the Chemical Composition and Anti-Tumor Mechanisms of Fruit By-Products: Based on LC-MS, Network Pharmacology Analysis, and Protein Target Validation.

(Lour.) Skeels, commonly known as Wampee, are valued for their edible and medicinal qualities, yet their pericarp and seeds are often discarded, resulting in wasted resources. This study investigates the anti-tumor potential of these by-products, focusing on their chemical composition and underlying mechanisms of action.

Research progress of paclitaxel nanodrug delivery system in the treatment of triple-negative breast cancer.

Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer, characterized by the loss or low expression of estrogen receptor (ER), human epidermal growth factor receptor 2 (HER2) and progesterone receptor (PR). Due to the lack of clear therapeutic targets, paclitaxel (PTX) is often used as a first-line standard chemotherapy drug for the treatment of high-risk and locally advanced TNBC. PTX is a diterpenoid alkaloid extracted and purified from Taxus plants, functioning as an anticancer agent by inducing and promoting tubulin polymerization, inhibiting spindle formation in cancer cells, and preventing mitosis.

Mediators of Filgotinib Treatment Effects in Ulcerative Colitis: Exploring Circulating Biomarkers in the Phase 2b/3 SELECTION Study.

Background: We utilized patient samples from the large, phase 2b/3 SELECTION trial to identify circulating biomarkers of ulcerative colitis (UC) and potential early mediators of filgotinib treatment effects.

Methods: Samples were collected at baseline and during the induction phase of the SELECTION trial. Evaluated biomarkers comprised serum and stool proteins (measured by enzyme-linked immunosorbent assay), whole-blood cell counts, and whole-blood RNA-seq-derived gene-expression factors identified via exploratory factor analysis.

Simultaneous elimination mechanism of formaldehyde and acrolein by resveratrol in food and the cytotoxicity of the products.

Polyphenols have been intensively investigated for scavenging single harmful aldehydes, such as formaldehyde (FA) and acrolein (ACR). However, there is a lack of studies on the effect and mechanism of eliminating co-existing harmful aldehydes by polyphenols. In this study, resveratrol (RV) was found to simultaneously scavenge FA and ACR by forming various adducts, with the RV-ACR adduct (RA, molecular formula: CHO) and RV-ACR-FA adduct (RAF, molecular formula: CHO) being the dominant ones.

Refining Biologically Inconsistent Segmentation Masks with Masked Autoencoders.

Microscopy images often feature regions of low signal-to-noise ratio (SNR) which leads to a considerable amount of ambiguity in the correct corresponding segmentation. This ambiguity can introduce inconsistencies in the segmentation mask which violate known biological constraints. In this work, we present a methodology which identifies areas of low SNR and refines the segmentation masks such that they are consistent with biological structures.

Feasibility and safety of a cable transmission magnetically controlled capsule endoscopy system for examination of the human upper digestive tract.

Objectives: To evaluate the safety and feasibility of the cable-transmitted, magnetically controlled capsule endoscopy (CT-MCCE) system for examining the esophagus and stomach.

Methods: A pilot study was carried out at the endoscopy facility of Peking University First Hospital; 30 volunteers were enrolled between September and November 2022. All participants were instructed to swallow the CT-MCCE capsule.

The effectiveness of a nurse-led home-based cardiac rehabilitation based on instant information intervention in the prognosis of patients with TAVR: A randomized controlled trial protocol.

Background: Patients with poor prognosis after TAVR surgery require high-quality cardiac rehabilitation. Digital home-based rehabilitation based on real-time information intervention can improve outcomes. However, the parameters of the text message intervention, such as text message content, intervention duration, text message frequency, and other parameters, may vary.

Based on computer simulation and experimental verification: mining and characterizing novel antimicrobial peptides from soil microbiome.

Antimicrobial peptides (AMPs) show great promise for enhancing food safety and extending shelf life, but traditional screening methods are complex and costly. To address these issues, we developed a deep learning-based prediction pipeline to identify potential AMPs from soil metagenomic data, achieving high accuracy (92.71 %) and precision (91.

Deep learning methods for high-resolution microscale light field image reconstruction: a survey.

Deep learning is progressively emerging as a vital tool for image reconstruction in light field microscopy. The present review provides a comprehensive examination of the latest advancements in light field image reconstruction techniques based on deep learning algorithms. First, the review briefly introduced the concept of light field and deep learning techniques.

Sodium formate induces development-dependent intestinal epithelial injury via necroptosis and apoptosis.

Objectives: Necrotizing enterocolitis (NEC) is a common and sometimes fatal disease affecting premature infants. Elevated formate has been found in the stool of patients with NEC. Sodium formate (NaF) is used to explore the role of formate in the intestinal epithelial injury.

Adipose tissue macrophages-derived exosomal MiR-500a-5p under high glucose promotes adipocytes inflammation by suppressing Nrf2 expression.

Background: Type 2 diabetes (T2DM) is a chronic metabolic disorder characterized by insulin resistance and chronic inflammation. Adipose tissue macrophages (ATMs), central players in mediating pro-inflammatory responses within adipose tissue, have been shown to influence insulin sensitivity through exosome secretion. While the role of macrophages-derived exosomal miRNA has been studied in various diseases, their pathogenic roles in T2DM, particularly ATMs-derived exosomal miRNA in adipose tissue inflammation, remain underexplored.

Design optimization of bimetal-modified biochar for enhanced phosphate removal performance in livestock wastewater using machine learning.

Mg-modified biochar shows high adsorption performance under weakly acidic and neutral water conditions. However, its phosphate removal efficiency markedly decreases in naturally alkaline wastewater, such as that released in livestock farming (anaerobic wastewater with a high phosphate concentration). This research employed six machine learning models to predict and optimize the phosphate removal performance of bimetal-modified biochar (i.

Design and performance of a novel multi-leaf collimator composed of leaves with fixed and movable layers.

Background: The multi-leaf collimator (MLC) is an advanced device utilized for beam shaping and intensity modulation in radiotherapy. With the framework of the contemporary single-layer MLC featuring a rounded leaf tip, the leaf tip transmission and leakage exert a considerable influence on radiotherapy.

Purpose: To scale down the leakage and transmission from the leaf gap when the opposite leaves are closed.

Automatic jawbone structure segmentation on dental CBCT images via deep learning.

Objectives: This study developed and evaluated a two-stage deep learning-based system for automatic segmentation of mandibular cortical bone, mandibular cancellous bone, maxillary cortical bone and maxillary cancellous bone on cone beam computed tomography (CBCT) images.

Materials And Methods: A dataset containing 155 CBCT scans acquired with different parameters was obtained. A two-stage deep learning-based system was developed for automatically segmenting jawbone structures.