[Simultaneous determination of 14 mycotoxins in Bombyx Batryticatus and Eupolyphaga Steleophaga by QuEChERS combined with UPLC-MS/MS].

A method for simultaneous determination of multiple mycotoxins in Bombyx Batryticatus and Eupolyphaga Steleophaga was established to provide technical support for monitoring and quality control of such medicinal materials. The mildew-prone Bombyx Batryticatus and Eupolyphaga Steleophaga were detected by UPLC-MS/MS technology, and the chromatographic conditions, mass spectrometry conditions, test sample preparation methods, and QuEChERS purification parameters were optimized. Scanning was conducted under multiple reaction monitoring mode(MRM), and quantitative analysis was performed by matrix standard curve method.

Simulation of distribution in China: assessing habitat suitability and bioactive component abundance under future climate change scenariosplant components.

Background: is used in traditional Chinese medicine, so ensuring an adequate supply of plant material with high levels of bioactive components is important.

Methods: Using an optimized maximum entropy niche model and assays of bioactive components from cultivation samples, this study started from the plant's natural distribution area and estimated correlations of ecological factors with not only abundance of the plant but also abundance of polysaccharides and heterophyllin B. These correlations were combined with the spatial analysis function in ArcGIS to generate maps of the suitability of different habitats in China for cultivating under current climate conditions and different models of climate change.

Identification and characterization of Morganella morganii strain YC12-C3 and Enterococcus faecalis strain YC12-C10 and elucidation of its deoxynivalenol-degrading potential.

Deoxynivalenol ( DON) is one of the most harmful mycotoxins in food or feed or Traditional Chinese Medicine. An efficient and applicable method for the detoxification of DON is urgently developed. 1152 strains were isolated from the intestinal contents of crucian.

Variation in SSRs at different genomic regions and implications for the evolution and identification of Armillaria gallica.

Armillaria spp. are devastating forest pathogens. Due to its low pathogenicity and abundant genetic variation, Armillaria gallica exhibited a unique and beneficial symbiosis with Gastrodia elata, which was used as a traditional Chinese medicine.

Evaluation of mycotoxins, mycobiota and toxigenic fungi in the traditional medicine .

Medicinal herbs have been increasingly used for therapeutic purposes against a diverse range of human diseases worldwide. However, inevitable contaminants, including mycotoxins, in medicinal herbs can cause serious problems for humans despite their health benefits. The increasing consumption of medicinal plants has made their use a public health problem due to the lack of effective surveillance of the use, efficacy, toxicity, and quality of these natural products.

Aflatoxin B1 contamination reduces the saponins content and anti-osteoporosis efficacy of the traditional medicine Radix Dipsaci.

Ethnopharmacological Relevance: The Radix Dipsaci, a traditional Chinese medicine with a history spanning over 2000 years in China, is widely recognized for its hepatorenal tonic properties, musculoskeletal fortifying effects, fracture healing capabilities, and its frequent application in the treatment of osteoporosis. Like many traditional Chinese herbal medicines, preparations from Radix Dipsaci are at risk of contamination by harmful mycotoxins such as aflatoxin B1.

Aims Of The Study: This study aims to evaluate the impact of aflatoxin B1 contamination on Radix Dipsaci in terms of changes in quality, efficacy of anti-osteoporosis and hepatorenal toxicity.

Simultaneous detection of multiple mycotoxins in Radix Dipsaci and estimation of exposure risk for consumers.

Like many traditional Chinese herbal medicines, preparations from Radix Dipsaci are at risk of contamination by harmful mycotoxins; however, there have been no reports of actual contamination. In this study, we developed an analytical method to simultaneously detect eight mycotoxins in Radix Dipsaci and estimate the exposure risk for consumers. We have developed an analytical method utilizing ultra-high performance liquid chromatography and tandem mass spectrometry to accurately determine the levels of AFB1, AFB2, AFG1, AFG2, OTA, ZEN, T-2 and ST mycotoxins in 45 batches of Radix Dipsaci sourced from major medicinal herb markets across five regions in China.

Pathogen-driven Pseudomonas reshaped the phyllosphere microbiome in combination with Pseudostellaria heterophylla foliar disease resistance via the release of volatile organic compounds.

Background: Continuous monocropping obstacles are common in plants, especially medicinal plants, resulting in disease outbreaks and productivity reductions. Foliar disease, mainly caused by Fusarium oxysporum, results in a severe decrease in the yield of Pseudostellaria heterophylla annually. Determining an effective biomethod to alleviate this disease is urgently needed to improve its productivity and quality.

Contamination of the traditional medicine Radix Dipsaci with aflatoxin B1 impairs hippocampal neurogenesis and cognitive function in a mouse model of osteoporosis.

Background: Aflatoxin B1, which can penetrate the blood-brain barrier and kill neural cells, can contaminate traditional herbal medicines, posing a significant risk to human health. The present study examined cellular, cognitive and behavioral consequences of aflatoxin B1 contamination of the anti-osteoporotic medicine Radix Dipsaci.

Methods: A mouse model of osteoporosis was created by treating the animals with all-trans-retinoic acid.

The sweating process promotes toxigenic fungi expansion and increases the risk of combined contamination of mycotoxins in .

Sweating is one of the most important processing methods of Chinese medicinal herbs. However, the high temperature and humidity environment required for sweating Chinese medicinal herbs makes it very easy for fungi to breed, especially toxigenic fungi. The mycotoxins produced by these fungi will then contaminate the Chinese medicinal herbs.

Composition and diversity of soil microbial communities change by introducing Phallus impudicus into a Gastrodia elata Bl.-based soil.

Background: The Gastrodia elata Bl. is an orchid, and its growth demands the presence of Armillaria species. The strong competitiveness of Armillaria species has always been a concern of major threat to other soil organisms, thus disrupting the equilibrium of soil biodiversity.

causes the leaf rot disease of in Guizhou, China.

Epimedium sagittatum is a collective term for herbaceous plants belonging to the family Berberidaceae. Their dried leaves and stems have significant therapeutic effects on tumor inhibition, hypertension control, and coronary heart disease (Ke et al. 2023; Zhao et al.

Gut microbiota regulate stress resistance by influencing microglia-neuron interactions in the hippocampus.

Communication among the brain, gut and microbiota in the gut is known to affect the susceptibility to stress, but the mechanisms involved are unclear. Here we demonstrated that stress resistance in mice was associated with more abundant and in the gut, but less abundant and . Stress-sensitive animals showed higher permeability and stronger immune responses in their colon, as well as higher levels of pro-inflammatory cytokines in serum.

A Probiotic D-1 Strain Is Responsible for Zearalenone Detoxifying in Coix Semen.

Zearalenone (ZEN) is a mycotoxin produced by spp., which commonly and severely contaminate food/feed. ZEN severely affects food/feed safety and reduces economic losses owing to its carcinogenicity, genotoxicity, reproductive toxicity, endocrine effects, and immunotoxicity.

Combined biochar and water-retaining agent application increased soil water retention capacity and maize seedling drought resistance in Fluvisols.

Global climate change has accelerated the occurrence of agricultural drought events, which threaten food security. Therefore, improvements in the soil water retention capacity (WRC) and crop drought resistance are crucial for promoting the sustainability of the agricultural environment. In this study, we explored the effects of applying biochar and water-retaining agent (WRA) on soil WRC and crop drought resistance in a Fluvisols, along with their potential mechanisms.

Effect of symbiotic fungi- on the yield of Bl. and insight into the response of soil microbial community.

members play important roles in the nutrient supply and growth modulation of Bl., and they will undergo severe competition with native soil organisms before colonization and become symbiotic with . Unraveling the response of soil microbial organisms to symbiotic fungi will open up new avenues to illustrate the biological mechanisms driving 's benefit from .

Comparative Analysis of the Expression of Resistance-Related Genes Respond to the Diversity Foliar Pathogens of Pseudostellaria heterophylla.

The foliar disease, which is the primary complex disease of Pseudostellaria heterophylla, can be caused by multiple co-infecting pathogens, resulting in a significant reduction in yield. However, there is a lack of research on the relationship between co-infection of various pathogens and the response of resistance-related genes in P. heterophylla.

[Cloning and functional verification of PhAEP gene, a key enzyme for biosynthesis of heterophyllin A in Pseudostellaria heterophylla].

This paper aimed to study the role of asparagine endopeptidase(AEP) gene in the biosynthesis mechanism of cyclic peptide compounds in Pseudostellaria heterophylla. The transcriptome database of P. heterophylla was systematically mined and screened, and an AEP gene, tentatively named PhAEP, was successfully cloned.

[Contamination status and exposure risk of mycotoxins in Coicis Semen].

By investigating the contamination status and predicting the exposure risk of mycotoxin in Coicis Semen, we aim to provide guidance for the safety supervision of Chinese medicinal materials and the formulation(revision) of mycotoxin limit standards. The content of 14 mycotoxins in the 100 Coicis Semen samples collected from five major markets of Chinese medicinal materials in China was determined by UPLC-MS/MS. The probability evaluation model based on Monte Carlo simulation method was established after Chi-square test and One-way ANOVA of the sample contamination data.

Volatiles from Strain B-BH16-1 Suppress Aflatoxin Production and Growth of s on during Storage.

Semen coicis is not only a traditional Chinese medicine (TCM), but also a typical food in China, with significant medical and healthcare value. Because semen coicis is rich in starch and oil, it can be easily contaminated with and its aflatoxins (AFs). Preventing and controlling the contamination of semen coicis with and its aflatoxins is vital to ensuring its safety as a drug and as a food.

Reversible Adhesive Bio-Toe with Hierarchical Structure Inspired by Gecko.

The agile locomotion of adhesive animals is mainly attributed to their sophisticated hierarchical feet and reversible adhesion motility. Their structure-function relationship is an urgent issue to be solved to understand biologic adhesive systems and the design of bionic applications. In this study, the reversible adhesion/release behavior and structural properties of gecko toes were investigated, and a hierarchical adhesive bionic toe (bio-toe) consisting of an upper elastic actuator as the supporting/driving layer and lower bionic lamellae (bio-lamellae) as the adhesive layer was designed, which can adhere to and release from targets reversibly when driven by bi-directional pressure.

Genome Resource for H4-3-C1: An Endophyte of with Degradation Activity to Toxins Produced by Fungal Pathogens.

is an endophyte of , a traditional Chinese herbal plant. It has high degradation activity to toxins produced by fungal pathogen . Here, we deployed PacBio single-molecule real-time long-read sequencing technology to generate a complete genome assembly for the H4-3-C1 strain and obtained 1.

Gasdermin D-mediated microglial pyroptosis exacerbates neurotoxicity of aflatoxins B1 and M1 in mouse primary microglia and neuronal cultures.

Aflatoxin B1 (AFB1) disrupts the blood-brain barrier by poisoning the vascular endothelial cells and astrocytes that maintain it. It is important to examine whether aflatoxin B1 or its metabolite, aflatoxin M1 (AFM1), affect microglia, which as the "immune cells" in the brain may amplify their damaging effects. Here we evaluated the toxicity of AFB1 and AFM1 against primary microglia and found that both aflatoxins decreased the viability of primary microglia and increased the leakage of lactate dehydrogenase, gamma-H2AX expression, nuclear lysis, necrosis and apoptosis in a dose-dependent manner.

[Construction of protoplast genetic transformation system for Mycena--symbiont of Gastrodia elata].

Mycena, a symbiont of Gastrodia elata, promotes seed germination of G. elata and plays a crucial role in the sexual reproduction of G. elata.