Fusing Events and Frames with Coordinate Attention Gated Recurrent Unit for Monocular Depth Estimation.

Monocular depth estimation is a central problem in computer vision and robot vision, aiming at obtaining the depth information of a scene from a single image. In some extreme environments such as dynamics or drastic lighting changes, monocular depth estimation methods based on conventional cameras often perform poorly. Event cameras are able to capture brightness changes asynchronously but are not able to acquire color and absolute brightness information.

Adherence to Mediterranean diet and female urinary incontinence: Evidence from the NHANES database.

Background: Urinary incontinence (UI) is a common condition in female. Oxidative stress and inflammation levels play important roles in UI progression. Mediterranean diet (MD) as a healthy anti-inflammatory dietary pattern has been reported to be associated with several inflammatory diseases.

Formation of Different Polyploids Through Disrupting Meiotic Crossover Frequencies Based on cntd1 Knockout in Zebrafish.

Polyploidy, a significant catalyst for speciation and evolutionary processes in both plant and animal kingdoms, has been recognized for a long time. However, the exact molecular mechanism that leads to polyploid formation, especially in vertebrates, is not fully understood. Our study aimed to elucidate this phenomenon using the zebrafish model.

Peri-implantation estradiol level has no effect on pregnancy outcome in vitro fertilization- embryo transfer.

Background: The necessity of monitoring luteal endocrine functions in in vitro fertilization- embryo transfer (IVF-ET) remains uncertain. Specifically, the significance of luteal phase estradiol (E2) levels is a matter of debate in current literature.

Objective: To assess the impact of luteal phase (day 11 after HCG trigger) estradiol levels on IVF-ET outcomes.

Ccdc57 is required for straightening the body axis by regulating ciliary motility in the brain ventricle of zebrafish.

Recently, cilia defects have been proposed to contribute to scoliosis. Here, we demonstrate that coiled-coil domain-containing 57 (Ccdc57) plays an essential role in straightening the body axis of zebrafish by regulating ciliary beating in the brain ventricle (BV). Zygotic ccdc57 (Zccdc57) mutant zebrafish developes scoliosis without significant changes in their bone density and calcification, and the maternal-zygotic ccdc57 (MZccdc57) mutant embryos display curved bodies since the long-pec stage.

A CT-based nomogram for differentiating invasive fungal disease of the lung from bacterial pneumonia.

Background: There is an annual increase in the incidence of invasive fungal disease (IFD) of the lung worldwide, but it is always a challenge for physicians to make an early diagnosis of IFD of the lung. Computed tomography (CT) may play a certain role in the diagnosis of IFD of the lung, however, there are no specific imaging signs for differentiating IFD of lung from bacterial pneumonia (BP).

Methods: A total of 214 patients with IFD of the lung or clinically confirmed BP were retrospectively enrolled from two institutions (171 patients from one institution in the training set and 43 patients from another institution in the test set).

The global incidence of adverse events associated with fecal microbiota transplantation in children over the past 20 years: A systematic review and meta-analysis.

Objectives: To understand the global incidence of the adverse events associated with fecal microbiota transplantation (FMT) in children over the past 20 years.

Methods: We searched PubMed, Web of Science, Embase, and three Chinese databases (CNKI, Wanfang, and Chongqing Weipu) for high-quality articles written over the past 20 years and made selections based on the quality standard score. The study characteristics and incidences of adverse events were extracted from each article, meta-analysis was performed using the R.

Quantitative measurement of cartilage volume with automatic cartilage segmentation in knee osteoarthritis.

Purpose: To determine the reproducibility of the automatic cartilage segmentation method using a prototype KneeCaP software (version 1.3; Siemens Healthcare, Erlangen, Germany) and to compare the difference in cartilage volume (CV) between the normal knee joint and knee osteoarthritis (KOA) of different degrees by using the above software.

Materials And Methods: The study included 62 subjects with knee OA and 29 healthy control subjects.

Pulmonary invasive fungal disease and bacterial pneumonia: a comparative study with high-resolution CT.

Background: Early diagnosis of invasive fungal disease (IFD) is challenging. High-resolution computed tomography (CT) may improve IFD diagnosis; however, there are no definitive imaging signs for differentiating between bacterial pneumonia and IFD.

Methods: We retrospectively evaluated CT images of 208 patients with IFD (n = 102) or bacterial pneumonia (n = 106).

Enzyme/pH-sensitive polyHPMA-DOX conjugate as a biocompatible and efficient anticancer agent.

In this study, to enhance the therapeutic function and reduce the side-effects of doxorubicin (DOX), a biodegradable N-(2-hydroxypropyl) methacrylamide (HPMA) polymer-DOX conjugate has been prepared through reversible addition fragmentation chain transfer (RAFT) polymerization and conjugation chemistry, and the anticancer agent DOX was covalently linked to the polymeric vehicle through a pH-responsive hydrazone bond. The cellular mechanisms of the conjugate were explored, and the therapeutic indexes were studied as well. The high molecular weight (MW) polymeric conjugate (94 kDa) was degraded into products with low MW (45 kDa) in the presence of lysosomal cathepsin B and also showed pH-responsive drug release behavior.

[Construction of a 15-plex Rapid STR Multiplex Amplification System].

Objective: To establish a 15-plex rapid STR multiplex amplification system.

Methods: Fourteen auto-chromosome loci and one sex-chromosome were selected to compare the situations of allelic losses and nonspecific amplication under different conditions. FastStart Taq DNA polymerase and DNA standard sample 9947A were used during amplification and optimization process.

Genetic structure and differentiation analysis of a Eurasian Uyghur population by use of 27 continental ancestry-informative SNPs.

Previously, we developed and validated a multiplex assay of 27 ancestry-informative markers (AIMs) for analyzing African (AFR), European (EUR), and East Asian (EAS) ancestry components. In this study, we typed and collectively analyzed a large Uyghur sample of 979 individuals to estimate the genetic coefficients of the 27 AIMs and investigate differentiation parameters between Uyghur and Han. The Uyghur allele frequencies ranged from 0.

Separation of sperm and epithelial cells based on the hydrodynamic effect for forensic analysis.

In sexual assault cases, forensic samples are a mixture of sperm from the perpetrator and epithelial cells from the victim. To obtain an independent short tandem repeat (STR) profile of the perpetrator, sperm cells must be separated from the mixture of cells. However, the current method used in crime laboratories, namely, differential extraction, is a time-consuming and labor-intensive process.

Construction of a library of cloned short tandem repeat (STR) alleles as universal templates for allelic ladder preparation.

Short tandem repeat (STR) genotyping methods are widely used for human identity testing applications, including forensic DNA analysis. Samples of DNA containing the length-variant STR alleles are typically separated and genotyped by comparison to an allelic ladder. Here, we describe a newly devised library of cloned STR alleles.

Population genetics analysis of 38 STR loci in the She population from Fujian Province of China.

A total of 38 autosomal Short Tandem Repeat (STR) loci (i.e., D5S818, D21S11, D7S820, CSF1P0, D2S1338, D3S1358, vWA, D8S1179, D16S539, Penta E, TPOX, TH01, D19S433, D18S51, FGA, D6S1043, D13S317, D12S391, D6S474, D12ATA63, D22S1045, D10S1248, D1S1677, D11S4463, D1S1627, D3S4529, D2S441, D6S1017, D4S2408, D17S1301, D1GATA113, D18S853, D20S482, D14S1434, D9S1122, D2S1776, D10S1435, D5S2500) were analyzed in the She population from Fujian Province of China with 154 unrelated individuals.

[Temporal and spatial variation of water nutrient level after exogenous nutrient input].

In order to study the spatial and temporal variations of nitrogen (N) and phosphorous (P) nutrition in artificial wetlands after a single exogenous nutrient input, 6 mosaic communities of 7 plant species were set up in a cement channel in the greenhouse. After the addition of N and P nutritional solutions, the concentrations of dissolved total nitrogen (DTN), dissolved total phosphorous (DTP), ammonia nitrogen (NH4(+)-N), nitrate nitrogen (NO3(-) -N) and nitrite nitrogen (NO2(-) -N) in the surface, middle, and bottom layers of the bulk water were determined regularly within 22 days. The results show that: (1) the water depth and measuring date have significant effects on nutritional contents while the type of plant communities have no such an influence; (2) the diffusion of nutrient from surface to the middle water layers is relatively slow, which costs 6 days under the current experimental condition; (3) in the bottom water layer, nutritional concentrations had no significant changes except for NO2-N, thus the exogenous nutrient input mainly affects the nutrient contents of surface and middle-level bulk water; (4) DTP and NH4(+) -N contents gradually decline to similar levels that before the nutritional input event until the end of experimental period, though DTN and NO3(-) -N content decrease much more slowly; (5) the fact that NO2(-) -N contents rise in water layers of all depths demonstrates that nitrification and denitrification in the process of N circulation are enhanced.

[Distribution of nitrogen in the sediment of Taige south river estuary].

To characterize the distribution of nitrogen in the sediment of Taige south river estuary, column samples of sediments were collected and analyzed in April, 2012. Our results revealed that a synchronized trend existed among the levels of ammonium nitrogen (NH4(+) -N), organic nitrogen (Org-N) and total nitrogen (TN) in the surface layer of sediment. The NH4(+) -N positively correlated with TN and Org-N significantly (P < 0.

Donor lymphocytes may acquire cytolytic specificity to donor's engrafted hematopoietic cells after a hematopoietic stem cell allograft resulting in marrow failure.

Background: Secondary rejection sometimes occurs after engraftment of allogeneic hematopoietic stem cell transplantation (allo-HSCT), which results in marrow failure. To clear possible reasons for BM failure, we observed a patient with acute myeloblastic leukemia who died of hematopoietic failure one year after apparently successful allo-HSCT.

Methods: The patient was a 44 year old male.

[Influence of perennial flooding and drought on growth restoration of Acorus calamus in water-level-fluctuation zone of the Three Gorges Reservoir].

Acorus calamus L. is a common kind of wetland plant species in the Three Gorges Reservoir. In this study, we investigated the influence of perennial flooding on growth restoration of A.

[Changes of T-cell clonality after induction-cultivation of peripheral T lymphocytes in adoptive immunotherapy for leukemias].

This study was purposed to analyze the changes of T-cell clonality after induction of peripheral T lymphocytes by autogenous DC and cytokines in the preparation of adoptive immunotherapy for leukemias. The bone marrow and peripheral blood from 21 leukemia patients at remission stage after treatment and subjected to adoptive immunotherapy were collected. Their DCs and T-cells were stimulated with cytokines and then were mixed to activate T-cells.

[Inhibition effect of diimide G-quadruplex ligand on proliferation of leukemia cells and its molecular mechanisms].

This study was aimed to investigate the growth inhibition effect of diimide G-quadruplex ligand on leukemia cells and to explore its molecular mechanisms. K562 leukemia cell lines were treated with various concentrations of the diimide G-quadruples ligand small molecule (0.1 - 10 micromol/L).

An improved design of PCR primers for detection of human T cell receptor beta chain repertoire.

Analysis of T cell receptor beta variable region (TCRBV) gene rearrangement is useful for clonal assessment of abnormal T cell proliferations in various diseases. However, most primer panels previously used can only amplify the third complementarity-determining region. Following IMGT database we established a panel of primers, which can amplify entire sequences of all functional TCRBV families.

TCR spectratyping revealed T lymphocytes associated with graft-versus-host disease after allogeneic hematopoietic stem cell transplantation.

Clonal expansion of T cells after allogeneic hematopoietic stem cell transplantation (allo-HSCT) has been observed, but their characteristics remain to be fully elucidated. We report here that CD8(+) T cells were the dominant T lymphocytes seen and T-cell repertoire diversity decreased dramatically during the first 3 months after allo-HSCT. Patients with GVHD grade II - IV had significantly lower T-cell repertoire diversity compared with non-GVHD patients.

[Real-time PCR array for simultaneous detection of 37 kinds of fusion genes in leukemia].

Objective: To develop a real-time PCR array for simultaneous quantitative detection of translocations/chromosomal aberrations in patients with leukemia, and to investigate the feasibility and utility thereof.

Methods: By construction and optimization a set of specific primes (totally 82 primers), an array containing 66 parallel PCR reactions was developed. That array was used on the specimens of bone marrow or peripheral blood from 31 patients with leukemia to detect simultaneously 37 fusion genes and 4 proto-oncogene activations often occurring in patients with leukemia.

Identification of disease-associated proteins by proteomic approach in ankylosing spondylitis.

Ankylosing spondylitis (AS) is a chronic systemic inflammatory disorder of the axial skeleton and shows significant inherited susceptibility. Auto-immune responses have been traditionally considered as a putative pathogenetic event in AS. However, no consistent self-antigen has been identified to responsible for the disorders in AS to this day.