Investigating T-Cell Receptor Dynamics Under In Vitro Antibody-Based Stimulation Using Imaging Flow Cytometry.

T cells play a pivotal role in the immune system's response to various conditions. They are activated by antigen-presenting cells (APCs) via T-cell surface receptors, resulting in cytokine production and T-cell proliferation. These interactions occur through the formation of immunological synapses.

Hydrolyzed soy protein nanoparticles designed for VD delivery: VD incorporation and remodeling behavior during digestion matters.

In this study, delivery efficiency of hydrolyzed soy protein nanoparticles (SNPs) with different loading patterns towards VD was investigated. Specifically, SNPs with hydrophobic regions feasible for VD loading (Pro1 and Pro2, Pattern1) showed better stabilization ability. While those co-assembled with VD (Pro3, Pattern2) showed weaker protection due to loosen structures.

Multi-Omics Analysis Reveals Impacts of LincRNA Deletion on Yeast Protein Synthesis.

Non-coding RNAs (ncRNAs) are widespread across various genomic regions and play a crucial role in modulating gene expression and cellular functions, thereby increasing biological complexity. However, the relationship between ncRNAs and the production of heterologous recombinant proteins (HRPs) remains elusive. Here, a yeast library is constructed by deleting long intergenic ncRNAs (lincRNAs), and 21 lincRNAs that affect α-amylase secretion are identified.

APMAT analysis reveals the association between CD8 T cell receptors, cognate antigen, and T cell phenotype and persistence.

Elucidating the relationships between a class I peptide antigen, a CD8 T cell receptor (TCR) specific to that antigen, and the T cell phenotype that emerges following antigen stimulation, remains a mostly unsolved problem, largely due to the lack of large data sets that can be mined to resolve such relationships. Here, we describe Antigen-TCR Pairing and Multiomic Analysis of T-cells (APMAT), an integrated experimental-computational framework designed for the high-throughput capture and analysis of CD8 T cells, with paired antigen, TCR sequence, and single-cell transcriptome. Starting with 951 putative antigens representing a comprehensive survey of the SARS-CoV-2 viral proteome, we utilize APMAT for the capture and single cell analysis of CD8 T cells from 62 HLA A*02:01 COVID-19 participants.

APMAT analysis reveals the association between CD8 T cell receptors, cognate antigen, and T cell phenotype and persistence.

Elucidating the relationships between a class I peptide antigen, a CD8 T cell receptor (TCR) specific to that antigen, and the T cell phenotype that emerges following antigen stimulation, remains a mostly unsolved problem, largely due to the lack of large data sets that can be mined to resolve such relationships. Here, we describe Antigen-TCR Pairing and Multiomic Analysis of T-cells (APMAT), an integrated experimental-computational framework designed for the high-throughput capture and analysis of CD8 T cells, with paired antigen, TCR sequence, and single-cell transcriptome. Starting with 951 putative antigens representing a comprehensive survey of the SARS-CoV-2 viral proteome, we utilize APMAT for the capture and single cell analysis of CD8 T cells from 62 HLA A*02:01 COVID-19 participants.

Transcriptomic Insights into Higher Anthocyanin Accumulation in 'Summer Black' Table Grapes in Winter Crop Under Double-Cropping Viticulture System.

Anthocyanins are responsible for grape ( L.) skin color. To obtain a more detailed understanding of the anthocyanin regulatory networks across' the summer and winter seasons in grapes under a double-cropping viticulture system, the transcriptomes of 'Summer Black' grapes were analyzed using RNA sequencing.

Enfortumab vedotin plus pembrolizumab for previously untreated locally advanced or metastatic urothelial carcinoma: a cost-effectiveness analysis.

Background: Both the antibody-drug conjugate (ADC) enfortumab vedotin (EV) and programmed death-1 inhibitor pembrolizumab have been shown to provide survival benefits in patients previously treated with locally advanced or metastatic urothelial carcinoma (la/mUC). Cost-effectiveness is necessary to consider whether the increased efficacy of the two therapies will lead to higher prices for first-line treatment of previously untreated la/mUC.

Objectives: To guide the choice of EV plus pembrolizumab or chemotherapy for patients with previously untreated la/mUC.

Integrating multi-omics features enables non-invasive early diagnosis and treatment response prediction of diffuse large B-cell lymphoma.

Background: Multi-omics features of cell-free DNA (cfDNA) can effectively improve the performance of non-invasive early diagnosis and prognosis of cancer. However, multimodal characterization of cfDNA remains technically challenging.

Methods: We developed a comprehensive multi-omics solution (COMOS) to specifically obtain an extensive fragmentomics landscape, presented by breakpoint characteristics of nucleosomes, CpG islands, DNase clusters and enhancers, besides typical methylation, copy number alteration of cfDNA.

Single-cell RNA-seq reveals as a prognostic biomarker in low-grade endometrial stromal sarcoma.

Background: Low-grade endometrial stromal sarcoma (LG-ESS) is a rare uterine malignancy characterized by its complex tumor microenvironment (TME) and high recurrence rates, posing challenges to accurate prognosis and effective treatment. Identifying prognostic biomarkers is essential for improving patient stratification and guiding therapeutic strategies.

Methods: Using single-cell transcriptome analysis combined with H&E and multiplex immunofluorescence staining, we identified a subpopulation of tumor cells in LG-ESS and further validated the association of this subpopulation and its characteristic genes with LG-ESS prognosis by molecular characterization and bulk transcriptome data.

Dental Coverage and Care When Transitioning From Medicaid to Medicare.

Importance: Millions of adults with low incomes lose Medicaid eligibility when transitioning to Medicare at age 65 years. However, it remains unclear how this transition is associated with dental care.

Objective: To examine the consequences of transitions from Medicaid to Medicare on coverage and use of dental services.

Laser explosion-based fast lithography to modulate silicon crystals.

Laser direct lithography allows for the maskless production and alignment of nanocrystals. However, laser-induced crystal growth requires a complex process of nucleation followed by growth that takes a long time, resulting in low efficiency. Here, we used a laser explosion to pre-grow silicon seed crystals in a silicon precursor solution, simplified crystal growth and increased the lithography efficiency.

Transport of miR-766-3p to A549 cells by plasma-derived exosomes and its effect on intracellular survival of Mycobacterium tuberculosis by regulating NRAMP1 expression in A549 cells.

Exosomal microRNAs (miRNAs) in circulation were recognized as potential biomarkers for the diagnosis of multiple diseases. However, its potential as a diagnostic hallmark for tuberculosis (TB) has yet to be explored. Here, we comprehensively analyze miRNA profiles in exosomes derived from the plasma of active TB patients and healthy persons to evaluate its efficacy in TB diagnosis.

Ultra-Stretchable Microfluidic Devices for Optimizing Particle Manipulation in Viscoelastic Fluids.

Viscoelastic microfluidics leverages the unique properties of non-Newtonian fluids to manipulate and separate micro- or submicron particles. Channel geometry and dimension are crucial for device performance. Traditional rigid microfluidic devices require numerous iterations of fabrication and testing to optimize these parameters, which is time-consuming and costly.

Injectable self-assembling peptide hydrogel as a promising vitreous substitute.

Vitreoretinal diseases pose significant threats to vision, often requiring vitrectomy and substitution of vitreous humor to restore ocular structure and visual function. However, existing substitutes have limitations that compromise patient outcomes. Supramolecular hydrogels, particularly peptide-based formulations, have emerged as promising alternatives due to their superior optical clarity, biocompatibility, and viscoelasticity.

Correlation between cognitive impairment and body composition indicators in patients with chronic kidney disease after hemodialysis.

Objective: To examine the relationship between cognitive impairment and body composition indicators in chronic kidney disease (CKD) patients post-hemodialysis.

Methods: This retrospective study included 110 CKD patients admitted to Beijing Luhe Hospital, Capital Medical University between January 2019 and January 2023. General clinical data and body composition indicators were compared between patients with and without cognitive impairment.

Mind the Particle Rigidity: Blooms the Bioavailability via Rapidly Crossing the Mucus Layer and Alters the Intracellular Fate of Curcumin.

Overcoming intestinal epithelial barriers to enhance bioavailability is a major challenge for oral delivery systems. Desirable nanocarriers should simultaneously exhibit rapid mucus penetration and efficient epithelial uptake; however, they two generally require contradictory structural properties. Herein, we proposed a strategy to construct multiperformance nanoparticles by modifying the rigidity of amphiphilic nanostructures originating from soy polypeptides (SPNPs), where its ability to overcome multibarriers was examined from both and , using curcumin (CUR) as a model cargo.

Analysis of risk factors for refractory peritonitis in patients undergoing peritoneal dialysis.

Objective: To explore the risk factors for refractory peritonitis in patients undergoing peritoneal dialysis.

Methods: We retrospectively collected data from 130 patients who underwent peritoneal dialysis (PD) and received peritonitis treatment at the Renal Disease Center of Beijing Luhe Hospital affiliated with Capital Medical University from January 1, 2016 to January 30, 2023. According to clinical treatment results, patients with refractory peritonitis were classified as the refractory group (n=52 cases), and those with non-refractory peritonitis were classified as the non-refractory group (n=78 cases).

Synthesis of Rare Earth Metal Complexes Stabilized by Amine Bridged Bis(phenolato) Ligands and Their Performance in the Polymerization of rac-β-Butyrolactone.

A series of rare earth alkoxides bearing amine-bridged bis(phenolato) ligands were synthesized through sequential reactions of RE(CH)(THF) (RE = Y, Lu) or Nd[N(SiMe)] with bis(phenols) LH and CFCHOH. Complexes REL(OCHCF)(THF) (1-6) bearing different aryl-substituents were obtained in good yields of 59-70 %. They were applied in the ring-opening polymerization (ROP) of rac-β-butyrolactone (rac-BBL), which showed good activity (TOF up to 27,300 h), resulting in syndiotactically enriched poly(3-hydroxybutyrate) (PHB) (P up to 0.

A novel visible light-driven oxygen doped CN/BiOCl/ferrate(VI) system for Bisphenol A degradation: Radical and nonradical pathways.

In this study, visible light-activated photocatalyst oxygen-doped CN@BiOCl (OCN@BOC) and Fe(VI) coupling system was proposed for the efficient degradation of bisphenol A (BPA). The comprehensive characterization of the OCN@BOC photocatalyst revealed its excellent photogenerated carrier separation rate in heterogeneous structures. The OCN@BOC/Fe(VI)/Vis system exhibited a remarkable BPA removal efficiency of over 84% within 5 min.