Genome sequence of a new densovirus infecting the leaf-curl plum aphid brachycaudus helichrysi.

Using degenerate primers and an overlap PCR approach, we identified a new densovirus (family Parvoviridae, subfamily Densovirinae) in an asexual clone of the leaf-curl plum aphid, Brachycaudus helichrysi, collected in Baoji, China, in June 2021. A nearly complete genome sequence of 5,551 nucleotides (nt) was obtained. The virus, with the proposed name "Brachycaudus helichrysi densovirus" (BhDV), has an ambisense genome organization with two ORFs in the sense strand encoding the nonstructural proteins NS1 and NS2 and one ORF at the antisense strand encoding the virion capsid protein VP.

Migration of wheat stripe rust from the primary oversummering region to neighboring regions in China.

Changing climate and changes in cropping systems have greatly affected outbreaks of plant diseases. Wheat stripe rust is a disease posing a threat to global wheat production, caused by Puccinia striiformis f. sp.

Cross-subject EEG-based Motor Imagery Recognition for Patient's Rehabilitation.

Motor imagery (MI), a kind of psychological representation without actual action, has garnered increasing attention in rehabilitation. However, the inherent differences between patients and healthy persons hinder rehabilitation by reducing the accuracy of cross-subject MI recognition. Although unsupervised domain adaptation (UDA) methods have mitigated individual differences, they still suffer from challenges in terms of selecting confusing source domains and accurately classifying MI samples at the boundary.

Comparative Effectiveness and Safety of the JAK Inhibitors and Biologic Disease-Modifying Antirheumatic Drugs in Treating Children With Nonsystemic Juvenile Idiopathic Arthritis: A Bayesian Meta-Analysis of Randomized Controlled Trials.

Objective: We evaluated the efficacy and safety profiles of JAK inhibitors (JAKi) and biologic disease-modifying antirheumatic drugs (bDMARDs) when used with or without methotrexate (MTX) for the treatment of nonsystemic forms of juvenile idiopathic arthritis (nsJIA).

Methods: Randomized clinical trials (RCTs) investigating efficacy and safety outcomes of JAKi or bDMARDs for the nsJIA population up to 2023 were searched in ClinicalTrial.gov, PubMed, EMBASE, and Cochrane databases.

Tim-3 promotes viral infection by suppressing the USP25-TRAF3-IRF7 signaling pathway.

Tim-3, an immune checkpoint inhibitor, plays key roles in maintaining immune homeostasis and is involved in viral evasion. However, the precise role of Tim-3 in viral infection remains to be determined. USP25 is a deubiquitinating enzyme that initiates antiviral immunity by deubiquitinating TRAF3 and triggering the antiviral signaling pathway.

A Joint Approach for Energy Replenishment and Data Collection with Two Distinct Types of Mobile Chargers in WRSN.

Wireless rechargeable sensor networks (WRSNs) address the energy scarcity problem in wireless sensor networks by introducing mobile chargers (MCs) to recharge energy-hungry sensor nodes. Scheduling MCs to charge the recharge nodes is the primary focus of the energy replenishment scheme in WRSNs. The performance of the energy replenishment scheme is significantly influenced by the energy level of each node, which is depends on the data collection scheme employed by the network.

Comprehensive Extraction and Biological Activities of Mycosporine-like Amino Acids and Glyceroglycolipids Extracts from Two Macroalgae and .

Mycosporine-like amino acids (MAAs) and glyceroglycolipids have promising applications in various fields, but limited research exists on their simultaneous extraction from macroalgae. This study optimized the key parameters (liquid-solid ratio, extraction temperature and extraction time) in the extraction of MAAs and glyceroglycolipids from and using single factor and response surface experiments. The yields of MAAs from and were 169.

A practical DNA data storage using an expanded alphabet introducing 5-methylcytosine.

Unlabelled: The DNA molecule is a promising next-generation data storage medium. Recently, it has been theoretically proposed that non-natural or modified bases can serve as extra molecular letters to increase the information density. However, this strategy is challenging due to the difficulty in synthesizing non-natural DNA sequences and their complex structure.

MPEMDA: A multi-similarity integration approach with pre-completion and error correction for predicting microbe-drug associations.

Exploring the associations between microbes and drugs offers valuable insights into their underlying mechanisms. Traditional wet lab experiments, while reliable, are often time-consuming and labor-intensive, making computational approaches an attractive alternative. Existing similarity-based machine learning models for predicting microbe-drug associations typically rely on integrated similarities as input, neglecting the unique contributions of individual similarities, which can compromise predictive accuracy.

Nitrogen-doped reduced graphene oxide for high efficient adsorption of methylene blue.

A highly efficient and widely applicable adsorbent for the removal of methylene blue (MB) was created using nitrogen-doped and reduced graphene oxide (NRGO). The effects of NRGO mass, pH, contact time, and the initial MB concentration on the adsorption properties of MB onto NRGO were investigated. The results showed that the adsorption behavior remained stable within the pH range of 2.

Assembly of 3-(Tetrazol-5-yl)triazole Complexes with Ammonium Perchlorate for High-Performance Energetic Composites.

Advanced energetic composites possess promising properties and wide-ranging applications in explosives and propellants. Nonetheless, most metal-based energetic composites present significant challenges due to surface oxidation and low-pressure output. This study introduces a facile method to develop energetic composites Cutztr@AP through the intermolecular assembly of nitrogen-rich energetic coordination polymers and high-energy oxidant ammonium perchlorate (AP).

Design, Synthesis, and Biological Activity Evaluation of 5-Aryl-cyclopenta[]pyridine Derivatives.

Taking the natural product cerbinal as the lead compound, 30 novel 5-aryl-cyclopenta[]pyridine derivatives were designed and synthesized based on the previous bioactivity studies of the cyclopenta[]pyridines. The modification of the position-5 of compound was achieved by amination, bromination, and cross coupling using cerbinal as the raw material. The results of the bioactivity tests demonstrated that partial compounds exhibited superior activity against plant viruses compared to compound .

LAPTM5 Confers the Resistance to Venetoclax via Promoting the Autophagosome-Lysosome Fusion in Multiple Myeloma.

Multiple myeloma (MM) is a haematological lymphoid malignancy marked by significant morbidity due to severe complications. Despite advances in targeted therapies, including proteasome inhibitors and the BCL-2 inhibitor venetoclax, drug resistance frequently occurs, with the underlying mechanisms poorly understood. This study investigates the role of lysosome-associated protein transmembrane 5 (LAPTM5) in conferring resistance to venetoclax in relapsed MM.

CRMP2 regulates mossy fiber sprouting and modulates microtubule dynamics in a pilocarpine induced rat model of epilepsy.

Objective: Our study aimed to investigate the role of CRMP2 in mossy fiber sprouting (MFS) using a pilocarpine-induced rat model of epilepsy.

Methods: First, the rats were sacrificed on the 1, 7, 14 and 28 day after pilocarpine injection. Quantitative Real-time PCR (qPCR) and Western blot (WB) were performed to assess mRNA and protein levels in the hippocampus and cortex.

Development and application of a LC-HRMS method for simultaneous determination of ten illicit antifungal drugs in herbal bacteriostatic products.

The widespread use of sub-therapeutic antibiotics may lead to treatment failures, increased resistance to antibiotics, and even the encouragement of the formation of superbugs. Fraudulent herbal medicines that actually contain unlabeled active ingredients are a global problem. Therefore, streamlined and accurate analytical techniques that can identify and quantify a variety of antimicrobials in suspected illegal products are required.

Antagonistic Effect of Nitrate Conversion on Photocatalytic Reduction of Aqueous Pertechnetate and Perrhenate.

Sustainable photocatalysis can effectively reduce the radioactive TcO to less soluble TcO·HO(s), but the reduction efficiency is highly susceptible to coexisting nitrate (NO). Here, we quantitatively investigate photocatalytic remediation conditions for Tc-contaminated water stimulated by the analogue perrhenate (ReO) in the presence of NO, and we elucidate the influence mechanism of NO by in situ characterizations. The interfering NO can compete with Re(VII) for the carbonyl radical (·CO) produced by formic acid (HCOOH) oxidation to generate nitrogen-containing products such as NH, NO, and NO, resulting in the decrease in the Re(VII) reduction ratio.

Ubiquitination and degradation of MHC-II by Tim-3 inhibits antiviral immunity.

We previously reported that Tim-3, an immune checkpoint inhibitor, inhibits MHC-II expression, but the molecular mechanisms involved and the implications for antiviral immunity remain to be determined. Here, we found that during H1N1 infection, Tim-3 inhibits MHC-II expression in macrophages/microglia in vitro. Tim-3 interacts with MHC-II via its intracellular tail and induces proteasomal dependent degradation of MHC-II.

Deep learning enables the use of ultra-high-density array in DNBSEQ.

DNBSEQ employs a patterned array to facilitate massively parallel sequencing of DNA nanoballs (DNBs), leading to a considerable boost in throughput. By employing the ultra-high-density (UHD) array with an increased density of DNB binding sites, the throughput of DNBSEQ can be further expanded. However, the typical imaging system of the DNBSEQ sequencer is unable to resolve adjacent DNBs spaced smaller than the resolution limit, resulting in poor base-calling performance of the UHD array and hindering its practical application.

Spatiotemporal modeling of molecular holograms.

Quantifying spatiotemporal dynamics during embryogenesis is crucial for understanding congenital diseases. We developed Spateo (https://github.com/aristoteleo/spateo-release), a 3D spatiotemporal modeling framework, and applied it to a 3D mouse embryogenesis atlas at E9.

A novel high-throughput quantitative method for the determination of per- and poly-fluoroalkyl substances in human plasma based on UHPLC-Q/Orbitrap HRMS coupled with isotope internal standard.

A novel method for the quantitative analysis of 56 per- and polyfluoroalkyl substances (PFASs) in human plasma was established on the basis of ultrahigh performance liquid chromatography tandem quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-Q/Orbitrap HRMS) in combination with accurate customized mass databases and isotopic internal standards. A streamlined, high-throughput, and high-recovery (RE) sample pretreatment method was developed. The method's performance was evaluated in terms of linearity, limit of quantification, RE, repeatability, reproducibility, and matrix effect.

Occurrence, distribution and health risk assessment of quinolone residues in cultured fish in southeast China.

Quinolone antibiotics are widely utilized in aquaculture, but little is known about the health effects of their residues. This study used UPLC-MS/MS to analyze the distribution of 11 quinolone antibiotics in cultured fish sold in a coastal city in southeast China - Fujian Province and to assess their health risks. The study found 35.