Shoutai pills improve the quality of oocytes exposed to the chemotherapeutic drug Hydroxyurea.

Hydroxyurea (HU), a DNA synthesis inhibitor, is one of the most common chemotherapeutic drugs that have been widely applied to treat a variety of cancers. HU treatment exhibits severe side effects including renal toxicity, skin toxicity and embryo-toxicity. However, the influence of HU on the female gamete development has not yet fully clarified.

Cell Cycle Progression Determines Wing Morph in the Polyphenic Insect Nilaparvata lugens.

Wing polyphenism is a phenomenon in which one genotype can produce two or more distinct wing phenotypes adapted to the particular environment. What remains unknown is how wing pad development is controlled downstream of endocrine signals such as insulin and JNK pathways. We show that genes important in cellular proliferation, cytokinesis, and cell cycle progression are necessary for growth and development of long wings.

Construction of Bimetallic Selenides Encapsulated in Nitrogen/Sulfur Co-Doped Hollow Carbon Nanospheres for High-Performance Sodium/Potassium-Ion Half/Full Batteries.

Metallic selenides have been widely investigated as promising electrode materials for metal-ion batteries based on their relatively high theoretical capacity. However, rapid capacity decay and structural collapse resulting from the larger-sized Na /K greatly hamper their application. Herein, a bimetallic selenide (MoSe /CoSe ) encapsulated in nitrogen, sulfur-codoped hollow carbon nanospheres interconnected reduced graphene oxide nanosheets (rGO@MCSe) are successfully designed as advanced anode materials for Na/K-ion batteries.

An Analysis of the Binding Function and Structural Organization of the Protein Corona.

Blood proteins adsorb onto the surface of nanoparticles after intravenous injection to form a protein corona. The underlying organization and binding function of these adsorbed proteins remain unclear. This can impact how the corona mediates cell and tissue interactions.

Berberine Protects Against Simulated Ischemia/Reperfusion Injury-Induced H9C2 Cardiomyocytes Apoptosis and Myocardial Ischemia/Reperfusion-Induced Apoptosis by Regulating the Mitophagy-Mediated HIF-1α/BNIP3 Pathway.

Berberine (BBR) has a variety of pharmacological activities and is widely used in Asian countries. However, the clinical application of BBR still lacks scientific basis, what protective mechanism of BBR against myocardial ischemia-reperfusion injury (MIRI). experiments, BBR pretreatment regulated autophagy-related protein expression, induced cell proliferation and autophagosome formation, and reduced the mitochondrial membrane potential (ΔΨm) increase in H9C2 cells.

Systemic Delivery of siRNA Specific for Silencing TLR4 Gene Expression Reduces Diabetic Cardiomyopathy in a Mouse Model of Streptozotocin-Induced Type 1 Diabetes.

Introduction: Diabetic cardiomyopathy is a cardiac dysfunction in patients with diabetes which may lead to overt heart failure and death. Toll-like receptor (TLR) signaling triggers diabetic cardiomyopathy through various mechanisms, one of which is the upregulation of TLR4 expression. The aim of this study was to delineate the role of TLR4 in diabetic cardiomyopathy.

Hippo/YAP Pathway Plays a Critical Role in Effect of GDNF Against Aβ-Induced Inflammation in Microglial Cells.

Neuroinflammation is a critical mechanism responsible for the progression of Alzheimer's disease (AD). Recent studies reveal that Hippo/Yes-associated protein (YAP) signaling pathway is highly associated with a series of inflammation-related disorders. Glial cell line-derived neurotrophic factor (GDNF), with its neurotrophic and anti-apoptotic functions for nervous system, has been demonstrated to decrease the expression of proinflammatory mediators.

Tungsten Diselenide Top-gate Transistors with Multilayer Antimonene Electrodes: Gate Stacks and Epitaxially Grown 2D Material Heterostructures.

We have demonstrated that with e-beam deposition of a thin AlO layer before atomic layer deposition, a uniform AlO film can be obtained on WSe/sapphire samples. Device performances are observed for WSe top-gate transistors by using oxide stacks as the gate dielectric. By using thermal evaporation, epitaxially grown multilayer antimonene can be prepared on both MoS and WSe surfaces.

Myeloid Cells Enriched for a Dendritic Cell Population From People Living With HIV Have Altered Gene Expression Not Restored by Antiretroviral Therapy.

Antiretroviral therapy (ART) for human immunodeficiency virus (HIV) infections has been designed to optimize CD4 T-cell survival and limit HIV replication. Cell types other than CD4 T cells such as monocytes/macrophage, dendritic cells, and granulocytes (collectively known as myeloid cells), are generally not considered in the development of ART protocols. Myeloid dendritic cells (mDCs) are the most potent inducers of CD4 T-cell activation and central to the regulation of immune responses.

Characteristics of the urinary microbiome in kidney stone patients with hypertension.

Background: Kidney stone disease (KSD) is more common in individuals with hypertension (HTN) than in individuals with normotension (NTN). Urinary dysbiosis is associated with urinary tract disease and systemic diseases. However, the role of the urinary microbiome in KSD complicated with HTN remains unclear.

Ore image segmentation method using U-Net and Res_Unet convolutional networks.

Image segmentation has been increasingly used to identify the particle size distribution of crushed ore; however, the adhesion of ore particles and dark areas in the images of blast heaps and conveyor belts usually results in lower segmentation accuracy. To overcome this issue, an image segmentation method UR based on deep learning U-Net and Res_Unet networks is proposed in this study. Gray-scale, median filter and adaptive histogram equalization techniques are used to preprocess the original ore images captured from an open pit mine to reduce noise and extract the target region.

Roles and correlation of FOXA1 and ZIC1 in breast cancer.

The aim of this study was to evaluate the prognostic role of Forkhead box A1 (FOXA1) in breast cancer and determine the relationship between FOXA1 and zinc finger of the cerebellum 1 (ZIC1). BCIP, GEPIA, and Oncomine databases were used to detect expression of FOXA1 and assess prognostic roles of FOXA1 and ZIC1 in invasive breast tumors. A total of 113 female invasive breast cancer cases were collected to investigate FOXA1 and ZIC1 expression via immunohistochemistry.

Berberine attenuates mitochondrial dysfunction by inducing autophagic flux in myocardial hypoxia/reoxygenation injury.

Berberine (BBR) is routinely prescribed in many Asian countries to treat diarrhea. Evidence from both animal and clinical investigations suggests that BBR exerts diverse pharmacological activities, including antidiabetic, antineoplastic, antihypertensive, and antiatherosclerotic effects. This study aimed to explore the cardioprotective mechanisms of BBR and to elucidate the modulations between autophagy and mitochondrial function during hypoxia/reoxygenation (H/R) in H9c2 cells.

Deficiency in the anti-apoptotic protein DJ-1 promotes intestinal epithelial cell apoptosis and aggravates inflammatory bowel disease via p53.

Parkinson disease autosomal recessive, early onset 7 (PARK7 or DJ-1) is involved in multiple physiological processes and exerts anti-apoptotic effects on multiple cell types. Increased intestinal epithelial cell (IEC) apoptosis and excessive activation of the p53 signaling pathway is a hallmark of inflammatory bowel disease (IBD), which includes ulcerative colitis (UC) and Crohn's disease (CD). However, whether DJ-1 plays a role in colitis is unclear.

Correction to: Cone-beam breast CT features associated with HER2/neu overexpression in patients with primary breast cancer.

The original version of this article, published on 03 January 2020, unfortunately contained two mistakes.

Ratio of the zygote cytoplasm to the paternal genome affects the reprogramming and developmental efficiency of androgenetic embryos.

Uniparental embryos have uniparental genomes and are very useful models for studying the specific gene expression of parents or for exploring the biological significance of genomic imprinting in mammals. However, the early developmental efficiency of androgenetic embryos is significantly lower than that of parthenogenetic embryos. In addition, oocytes are able to reprogram sperm nuclei after fertilization to guarantee embryonic development by maternally derived reprogramming factors, which accumulate during oogenesis.

Vitamin D receptor targets hepatocyte nuclear factor 4α and mediates protective effects of vitamin D in nonalcoholic fatty liver disease.

Epidemiological studies have suggested a link between vitamin D deficiency and increased risk for nonalcoholic fatty liver disease (NAFLD); however, the underlying mechanisms have remained unclear. Here, using both clinical samples and experimental rodent models along with several biochemical approaches, we explored the specific effects and mechanisms of vitamin D deficiency in NAFLD pathology. Serum vitamin D levels were significantly lower in individuals with NAFLD and in high-fat diet (HFD)-fed mice than in healthy controls and chow-fed mice, respectively.

[Genetic analysis of a patient with late infantile metachromatic leukodystrophy].

Objective: To detect variants of ARSA gene in a child featuring late infantile metachromatic leukodystrophy (MLD).

Methods: PCR and Sanger sequencing was carried out for the patient and her parents.

Results: The patient had typical features of MLD including ARSA deficiency, regression of walking ability, and demyelination.

Single-Atom Vacancy Defect to Trigger High-Efficiency Hydrogen Evolution of MoS.

Defect engineering is widely applied in transition metal dichalcogenides (TMDs) to achieve electrical, optical, magnetic, and catalytic regulation. Vacancies, regarded as a type of extremely delicate defect, are acknowledged to be effective and flexible in general catalytic modulation. However, the influence of vacancy states in addition to concentration on catalysis still remains vague.

Amplified Electrochemical Biosensing of Thrombin Activity by RAFT Polymerization.

As a serine protease, thrombin is a pivotal component in coagulation cascade and has been frequently screened as an informative biomarker for the diagnosis of coagulation disorder-related diseases. Herein, a "signal-on" electrochemical biosensor is described for the highly sensitive and selective detection of thrombin activity, by exploiting a thrombin-specific substrate peptide (Tb peptide) as the recognition element and reversible addition-fragmentation chain transfer (RAFT) polymerization for signal amplification. Specifically, the carboxyl-group-free Tb peptides are self-assembled onto gold electrode surface via the N-terminal cysteine residue and are used for the specific recognition of thrombin molecules.

Y-box protein 1 promotes hypoxia/reoxygenation- or ischemia/reperfusion-induced cardiomyocyte apoptosis via SHP-1-dependent STAT3 inactivation.

Cardiomyocyte apoptosis induced by hypoxia and ischemia plays important roles in heart dysfunction after acute myocardial infarction (AMI). However, the mechanism of apoptosis induction remains unclear. A previous study reported that Y-box protein 1 (YB1) is upregulated after myocardial hypoxia/reoxygenation or ischemia/reperfusion (H/R or I/R, respectively) injury; however, whether YB1 is associated with H/R-induced cardiomyocyte apoptosis is completely unknown.

DO-LPV-based robust 3D path following control of underactuated autonomous underwater vehicle with multiple uncertainties.

This study investigates a path following problem of underactuated autonomous underwater vehicle under multiple uncertainties in three-dimensional space. The uncertainties are partly induced by hydrodynamic coefficient uncertainty, ocean currents and unmodeled hydrodynamics, namely dynamic uncertainties. In addition, velocities are assumed to be measured with bounded noise, which is called velocity measurement uncertainties.

The entry of nanoparticles into solid tumours.

The concept of nanoparticle transport through gaps between endothelial cells (inter-endothelial gaps) in the tumour blood vessel is a central paradigm in cancer nanomedicine. The size of these gaps was found to be up to 2,000 nm. This justified the development of nanoparticles to treat solid tumours as their size is small enough to extravasate and access the tumour microenvironment.

Distribution of organophosphate esters between the gas phase and PM in urban Dalian, China.

We investigated the concentrations and seasonal variations of organophosphate esters (OPEs) in the gas phase and PM (particulate matter with an aerodynamic diameter <2.5 μm) in an urban area of Dalian, China, as well as their gas-particle partitioning. The total concentrations of OPEs in the gas phase were in the range of 0.