[Application progress of indocyanine green angiography in lymphedema].

Objective: To summarize the application progress of indocyanine green (ICG) angiography in diagnosis and treatment of lymphedema.

Methods: The literature related to dynamic imaging tracing of lymphedema at home and abroad was reviewed extensively. And the research status and progress of ICG angiography in diagnosis and treatment of lymphedema were retrospectively analyzed.

Fluorometric determination of fipronil by integrating the advantages of molecularly imprinted silica and carbon quantum dots.

A fluorometric method is described for the determination of fipronil, a frequently-used insecticide. It exploits the blue fluorescence of carbon quantum dots (CQDs) and the selectivity of molecularly imprinted silica (MIS). The MIS was prepared via the sol-gel method by using fipronil as the template, 3-aminopropyltriethoxysilane as functional monomer, and tetraethoxysilane as cross-linker in the presence of CQDs.

Separation performance of -butyl(tetradecyloxy)calix[6]arene as a stationary phase for capillary gas chromatography.

This work presents the first example of the utilization of -butyl(tetradecyloxy)calix[6]arene (C6A-C10) as a stationary phase for capillary gas chromatographic (GC) separation. The statically coated C6A-C10 column showed a column efficiency of 3293 plates per m and had a nonpolar nature. Its selectivity and retention behaviour were investigated using a number of mixtures of diverse analytes and their isomers.

Modified behavioural tests to detect white matter injury- induced motor deficits after intracerebral haemorrhage in mice.

Motor function deficit induced by white matter injury (WMI) is one of the most severe complications of intracerebral haemorrhage (ICH). The degree of WMI is closely related to the prognosis of patients after ICH. However, the current behavioural assessment of motor function used in the ICH mouse model is mainly based on that for ischaemic stroke and lacks the behavioural methods that accurately respond to WMI.

Study of gastrointestinal tract viability and motility via modulation of serotonin in a zebrafish model by probiotics.

The relationship between bacterial strains and serotonin regulation was studied via the in vitro screening of the strains. Using the transparent visibility of zebrafish, the strains for intestinal peristalsis were quickly screened. The methods and results were as follows: (1) 18 strains with outstanding gastrointestinal viability via their surface hydrophobicity, self-aggregation ability, acid tolerance, bile tolerance, simulated gastric digestion and intestinal fluid tolerance were determined from 54 unpublished strains.

A multifunctional gelatine-quaternary ammonium copolymer: An efficient material for reducing dye emission in leather tanning process by superior anionic dye adsorption.

Leather wastewater is one of the most polluting industrial emissions. The efficiency of wastewater remediation is limited by its complex composition. Herein, a novel strategy for designing modified gelatine with higher degree of quaternization (MG-2) is presented.

Surfactant-Sensitized Covalent Organic Frameworks-Functionalized Lanthanide-Doped Nanocrystals: An Ultrasensitive Sensing Platform for Perfluorooctane Sulfonate.

Perfluorooctane sulfonate (PFOS) known as a persistent organic pollutant has been attracting great interests due to its potential ecotoxicity. An approach capable of sensing ultra-trace PFOS is in urgent demand. Here, we developed an approach for highly sensitive sensing PFOS using surfactant-sensitized covalent organic frameworks (COFs)-functionalized upconversion nanoparticles (UCNPs) as a fluorescent probe.

SV40 Hijacks Cellular Transport, Membrane Penetration, and Disassembly Machineries to Promote Infection.

During entry, a virus must be transported through the endomembrane system of the host cell, penetrate a cellular membrane, and undergo capsid disassembly, to reach the cytosol and often the nucleus in order to cause infection. To do so requires the virus to coordinately exploit the action of cellular membrane transport, penetration, and disassembly machineries. How this is accomplished remains enigmatic for many viruses, especially for viruses belonging to the nonenveloped virus family.

Upregulated Expression of MicroRNA-204-5p Leads to the Death of Dopaminergic Cells by Targeting DYRK1A-Mediated Apoptotic Signaling Cascade.

MicroRNAs (miRs) downregulate or upregulate the mRNA level by binding to the 3'-untranslated region (3'UTR) of target gene. Dysregulated miR levels can be used as biomarkers of Parkinson's disease (PD) and could participate in the etiology of PD. In the present study, 45 brain-enriched miRs were evaluated in serum samples from 50 normal subjects and 50 sporadic PD patients.

Star-poly(ε-caprolactone) as the stationary phase for capillary gas chromatographic separation.

This work presents the separation performance of star-poly(ε-caprolactone) (star-PCL) as the stationary phase for capillary gas chromatography (GC). The statically coated star-PCL column showed a column efficiency of 3345 plates per m and moderate polarity. Importantly, the star-PCL column exhibited high selectivity and resolving capability for more than a dozen mixtures covering a wide-ranging variety of analytes and isomers.

Generation of induced pluripotent stem cells from a young-onset Parkinson's disease patient carrying the compound heterozygous PLA2G6 p.D331Y/p.M358IfsX mutations.

Mutations in PLA2G6 gene cause PLA2G6-associated neurodegeneration, including recessive familial type 14 of Parkinson's disease (PARK14). Previously, we identified PARK14 patients with compound heterozygous c.991G > T/c.

Amphiphilic Block Copolymer PCL-PEG-PCL as Stationary Phase for Capillary Gas Chromatographic Separations.

This work presents the first example of utilization of amphiphilic block copolymer PCL-PEG-PCL as a stationary phase for capillary gas chromatographic (GC) separations. The PCL-PEG-PCL capillary column fabricated by static coating provides a high column efficiency of 3951 plates/m for -dodecane at 120 °C. McReynolds constants and Abraham system constants were also determined in order to evaluate the polarity and possible molecular interactions of the PCL-PEG-PCL stationary phase.

Molecular Dynamics Simulations Reveal the Inhibitory Mechanism of Dopamine against Human Islet Amyloid Polypeptide (hIAPP) Aggregation and Its Destabilization Effect on hIAPP Protofibrils.

The aberrant self-assembly of human islet amyloid polypeptide (hIAPP) into toxic oligomers, protofibrils, and mature fibrils is associated with the pathogenesis of type 2 diabetes (T2D). Inhibition of hIAPP aggregation and destabilization of preformed hIAPP fibrils are considered as two major therapeutic strategies for treating T2D. Previous experimental studies reported that dopamine prevented the formation of hIAPP oligomers and fibrils.

Molecular Beam Epitaxy and Electronic Structure of Atomically Thin Oxyselenide Films.

Atomically thin oxychalcogenides have been attracting intensive attention for their fascinating fundamental properties and application prospects. Bi O Se, a representative of layered oxychalcogenides, has emerged as an air-stable high-mobility 2D semiconductor that holds great promise for next-generation electronics. The preparation and device fabrication of high-quality Bi O Se crystals down to a few atomic layers remains a great challenge at present.

The optoelectronic microrobot: A versatile toolbox for micromanipulation.

Microrobotics extends the reach of human-controlled machines to submillimeter dimensions. We introduce a microrobot that relies on optoelectronic tweezers (OET) that is straightforward to manufacture, can take nearly any desirable shape or form, and can be programmed to carry out sophisticated, multiaxis operations. One particularly useful program is a serial combination of "load," "transport," and "deliver," which can be applied to manipulate a wide range of micrometer-dimension payloads.

How calorie-rich food could help marine calcifiers in a CO-rich future.

Increasing carbon emissions not only enrich oceans with CO but also make them more acidic. This acidifying process has caused considerable concern because laboratory studies show that ocean acidification impairs calcification (or shell building) and survival of calcifiers by the end of this century. Whether this impairment in shell building also occurs in natural communities remains largely unexplored, but requires re-examination because of the recent counterintuitive finding that populations of calcifiers can be boosted by CO enrichment.

Performance and selectivity of lower-rim substituted calix[4]arene as a stationary phase for capillary gas chromatography.

This work presents the investigation of -butyl(tetradecyloxy)calix[4]arene (C4A-C10) as stationary phase for capillary gas chromatographic (GC) separations. The statically-coated C4A-C10 capillary column showed weak polarity and column efficiency of 2566 plates per m determined by -dodecane at 120 °C. Impressively, the C4A-C10 column exhibited extremely high resolving capability for a wide range of analytes from nonpolar to polar, including -alkanes, esters, ketones, aldehydes, alcohols and bromoalkanes.

All-dielectric metasurface grating for on-chip multi-channel orbital angular momentum generation and detection.

Optical vortices, carrying quantized orbital angular momentum (OAM) states, have been widely investigated because of their promising applications in both classical and quantum realms. Among these applications, efficient generation and measurement of OAM beams are critical. Current techniques available for generating OAM beams generally suffer from bulky size, low operation efficiency, or single-function or complicated fabrication processes.

Molecular dynamics simulations reveal the mechanism of graphene oxide nanosheet inhibition of Aβ peptide aggregation.

The aggregation of the amyloid-beta (Aβ) peptides into toxic β-sheet-rich oligomers, protofibrils and mature fibrils is the major pathological hallmark of Alzheimer's disease (AD). Inhibiting the β-sheet formation and fibrillization of Aβ peptides is considered an important treatment strategy for AD. Graphene oxide (GO) has attracted particular interest in the anti-aggregation of amyloid proteins due to its good ability of crossing the blood-brain barrier (BBB), low cytotoxicity and good biocompatibility.

Computed tomography angiography-based analysis of high-risk intracerebral haemorrhage patients by employing a mathematical model.

Background: Haemorrhagic stroke accounts for approximately 31.52% of all stroke cases, and the most common origin is hypertension. However, little is known about the method to identify high-risk populations of hypertensive intracerebral haemorrhage.

The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection.

Although flaviviruses co-opt the function of the host endoplasmic reticulum (ER) membrane protein complex (EMC) during infection, a mechanistic explanation for this observation remains unclear. Here, we show that the EMC promotes biogenesis of dengue virus (DENV) and Zika virus (ZIKV) non-structural multi-pass transmembrane proteins NS4A and NS4B, which are necessary for viral replication. The EMC binds to NS4B and colocalizes with the DENV replication organelle.

A Double-Buffering Strategy to Boost the Lithium Storage of Botryoid MnO /C Anodes.

Transition metal oxides (TMOs) are regarded as promising candidates for anodes of lithium ion batteries, but their applications have been severely hindered by poor material conductivity and lithiated volume expansion. As a potential solution, herein is presented a facile approach, by electrospinning a manganese-based metal organic framework (Mn-MOF), to fabricate yolk-shell MnO nanostructures within carbon nanofibers in a botryoid morphology. While the yolk-shell structure accomodates the lithiated volume expansion of MnO , the fiber confinement ensures the structural integrity during charge/discharge, achieving a so-called double-buffering for cyclic volume fluctuation.

Stereoselective Oxidation Kinetics of Deoxycholate in Recombinant and Microsomal CYP3A Enzymes: Deoxycholate 19-Hydroxylation Is an In Vitro Marker of CYP3A7 Activity.

The primary bile acids (BAs) synthesized from cholesterol in the liver are converted to secondary BAs by gut microbiota. It was recently disclosed that the major secondary BA, deoxycholate (DCA) species, is stereoselectively oxidized to tertiary BAs exclusively by CYP3A enzymes. This work subsequently investigated the in vitro oxidation kinetics of DCA at C-1, C-3, C-4, C-5, C-6, C-6, and C-19 in recombinant CYP3A enzymes and naive enzymes in human liver microsomes (HLMs).