MicroRNA miR-20a-5p targets CYCS to inhibit apoptosis in hepatocellular carcinoma.

Hepatocellular carcinoma is a primary liver cancer, characterised by diverse etiology, late diagnoses, and poor prognosis. Hepatocellular carcinoma is mostly resistant to current treatment options, therefore, identification of more effective druggable therapeutic targets is needed. We found microRNA miR-20a-5p is upregulated during mouse liver tumor progression and in human hepatocellular carcinoma patients.

[Relationship between serum procalcitonin level and severity and prognosis in patients with traumatic brain injury in plateau areas].

Objective: To analyze the changes rule of serum procalcitonin (PCT) levels in patients with traumatic brain injury in plateau areas, and to evaluate its value in assessing the severity and prognosis of the patients.

Methods: A prospective cohort study was conducted. The patients with traumatic brain injury admitted to the critical care medicine departments of Xining Third People's Hospital (at an altitude of 2 260 metres) and Golmud City People's Hospital (at an altitude of 2 780 metres) from May 2018 to September 2022 were enrolled.

Two-point Fixation for Biological Mesh in Laparoscopic Inguinal Hernia Repair.

Objective: To evaluate the clinical outcomes and safety of two-point fixation for biological mesh in laparoscopic totally extraperitoneal (TEP) inguinal hernia repair.

Study Design: Case series.

Place And Duration Of Study: The first Affiliated Hospital of Fujian Medical University Hospital, Fuzhou, China, from January to December 2019.

Using machine learning models to predict HBeAg seroconversion in CHB patients receiving pegylated interferon-α monotherapy.

Background And Objective: Though there are many advantages of pegylated interferon-α (PegIFN-α) treatment to chronic hepatitis B (CHB) patients, the response rate of PegIFN-α is only 30 ~ 40%. Therefore, it is important to explore predictors at baseline and establish models to improve the response rate of PegIFN-α.

Methods: We randomly divided 260 HBeAg-positive CHB patients who were not previously treated and received PegIFN-α monotherapy (180 μg/week) into a training dataset (70%) and testing dataset (30%).

Compartmentalisation of Hepatitis B virus X gene evolution in hepatocellular carcinoma microenvironment and the genotype-phenotype correlation of tumorigenicity in HBV-related patients with hepatocellular carcinoma.

Hepatitis B virus (HBV) exists as quasispecies (QS). However, the evolutionary characteristics of haplotypes of HBV X gene in the hepatocellular carcinoma (HCC) microenvironment remain unclear. Mutations across X gene are essential for the tumorigenicity of HBV X protein (HBx).

High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.

Alterations in glycosylation regulate fundamental molecular and cellular processes of cancer, serving as important biomarkers and therapeutic targets. However, the potential association and regulatory mechanisms of E6 oncoprotein on glycosylation of cervical cancer cells are still unclear. Here, we evaluated the glycomic changes using Lectin microarray and determined the corresponding enzymes associated with endogenous high-risk HPV16 E6 expression in cervical cancer cells.

N6-methylandenosine-related lncRNAs play an important role in the prognosis and immune microenvironment of pancreatic ductal adenocarcinoma.

Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive, fatal tumor. N6-methylandenosine (m6A) methylation is the major epigenetic modification of RNA including lncRNAs. The roles of m6A-related lncRNAs in PDAC have not been fully clarified.

Elderly Male With Cardiovascular-Related Comorbidities Has a Higher Rate of Fatal Outcomes: A Retrospective Study in 602 Patients With Coronavirus Disease 2019.

Elderly with comorbidities have shown a higher rate of fatal outcomes when suffering coronavirus disease 2019 (COVID-19). However, a delineation of clinical significances of hematologic indices and underlying comorbidities in the progression and outcome of COVID-19 remains undefined. Six hundred two COVID-19 patients with established clinical outcomes (discharged or deceased) from Hankou Hospital of Wuhan, China between January 14, 2020 and February 29, 2020 were retrospectively analyzed.

Effect of type 2 diabetic mellitus in the prognosis of acute-on-chronic liver failure patients in China.

Background: Acute-on-chronic liver failure (ACLF) patients have a high short-term mortality rate, and the severity evaluation of ACLF is necessary for prognostication. Therefore, it was meaningful to evaluate the association between type 2 diabetic mellitus (DM) and ACLF and further explore the feasibility of using DM as a prognostic indicator in ACLF patients. The association between type 2 DM and the prognosis of patients with severe liver disease remains unclear.

EDTA-K Improves the Detection Sensitivity of SARS-CoV-2 IgM and IgG Antibodies by Chelating Colloidal Gold in the Immunochromatographic Assay.

Objective: The coronavirus disease (COVID-19) pandemic, caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), is now rapidly spreading globally. Serological tests are an important method to assist in the diagnosis of COVID-19, used for epidemiological investigations. In this study, we aimed to investigate the impact of different types of vacuum collection tubes on the detection of SARS-CoV-2 IgM and IgG antibodies, using the colloidal gold immunochromatographic assay (GICA).

A facile and low-cost AlO coating as an artificial solid electrolyte interphase layer on graphite/silicon composites for lithium-ion batteries.

Graphite/silicon (G/Si) composites are considered as possible alternative anode materials to commercial graphite anodes. However, the unstable solid electrolyte interphase (SEI) on G/Si particles results in rapid capacity decay, impeding practical applications. Herein, a facile and low-cost AlO coating was developed to fabricate stable artificial SEI layers on G/Si composites.

Mutational characterization of HBV reverse transcriptase gene and the genotype-phenotype correlation of antiviral resistance among Chinese chronic hepatitis B patients.

The drug resistance of hepatitis B virus (HBV) originates from mutations within HBV reverse transcriptase (RT) region during the prolonged antiviral therapy. So far, the characteristics of how these mutations distribute and evolve in the process of therapy have not been clarified yet. Thus we aimed to investigate these characteristics and discuss their contributing factors.

Heat inactivation of serum interferes with the immunoanalysis of antibodies to SARS-CoV-2.

Background: The detection of serum antibodies to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is emerging as a new tool for the coronavirus disease 2019 (COVID-19) diagnosis. Since many coronaviruses are sensitive to heat, heating inactivation of samples at 56°C prior to testing is considered a possible method to reduce the risk of transmission, but the effect of heating on the measurement of SARS-CoV-2 antibodies is still unclear.

Methods: By comparing the levels of SARS-CoV-2 antibodies before and after heat inactivation of serum at 56°C for 30 minutes using a quantitative fluorescence immunochromatographic assay RESULTS: We showed that heat inactivation significantly interferes with the levels of antibodies to SARS-CoV-2.

Impact of heat-inactivation on the detection of SARS-CoV-2 IgM and IgG antibody by ELISA.

Background: To establish a safe and accurate method for detecting SARS-CoV-2 IgM and IgG, we assessed the impact of sera after heat-inactivation on the SARS-CoV-2 IgM and IgG levels measured by ELISA-immunoassay.

Methods: The serum samples of 62 patients with COVID-19 and 18 healthy controls were collected in Hankou's Hospital of Wuhan from February 27 to March 6, 2020. Before and after the samples were inactivated, the levels of IgM and IgG antibodies were measured.

Lentiviral-mediated microRNA-26b up-regulation inhibits proliferation and migration of hepatocellular carcinoma cells.

Hepatocellular carcinoma (HCC) is a frequently occurred malignancy worldwide with a high mortality. The treatment for HCC is still controversial. Emerging evidences have demonstrated that microRNAs (miRs) play a role in HCC.

The paradoxical role of tumor-infiltrating immune cells in lung cancer.

Lung cancer remains one of the leading causes of death worldwide, and lung cancers have often already metastasized when diagnosed. Numerous studies have noted the infiltration of immune cells in the lung cancer microenvironment, but these cells play a dualistic role, they suppress and/or promote tumor development and growth based on tumor progression and different cytokines in the microenvironment. These tumor-infiltrating immune cells create different microenvironments depending on their type and interaction.

Association between mitochondrial DNA content and baseline serum levels of HBsAg in chronic hepatitis B infection.

Recent studies have demonstrated a potential link between mitochondrial DNA (mtDNA) content and cirrhosis or hepatocellular carcinoma (HCC). However, there are few studies evaluating mtDNA content as a noninvasive marker of chronic hepatitis B infection (CHB). In this study, we conducted a case-control study to determine mtDNA content in peripheral blood leukocyte (PBL) samples from 76 CHB cases naïve to antivirus therapy and 96 healthy controls, and then evaluated the association between mtDNA content and baseline serum concentration of HBV markers.

Structurally tailored graphene nanosheets as lithium ion battery anodes: an insight to yield exceptionally high lithium storage performance.

How to tune graphene nanosheets (GNSs) with various morphologies has been a significant challenge for lithium ion batteries (LIBs). In this study, three types of GNSs with varying size, edge sites, defects and layer numbers have been successfully achieved. It was demonstrated that controlling GNS morphology and microstructure has important effects on its cyclic performance and rate capability in LIBs.

Influence of ferric oxide modification on the properties of copper oxide supported on gamma-alumina.

X-ray diffraction (XRD), Mössbauer spectroscopy, and temperature-programmed reduction (TPR) were employed to investigate the dispersion and reduction behaviors of the Fe(2)O(3)/CuO/gamma-Al(2)O(3) system. The results indicated that: (1) the crystalline CuO particle in the CuO/gamma-Al(2)O(3) samples was redispersed during impregnating CuO/gamma-Al(2)O(3) samples with Fe(NO(3))(3) solutions; (2) two different dispersion states of surface iron species could be observed, i.e.

A comparative study on the dispersion behaviors and surface acid properties of molybdena on CeO2 and ZrO2 (Tet).

Dispersion of molybdena on CeO(2), ZrO(2) (Tet), and a mixture of CeO(2) and ZrO(2) (Tet), was investigated by using laser Raman spectroscopy (LRS), Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD) and temperature programmed reduction (TPR). The results indicate that molybdena is dispersed on both individual oxide support and mixed oxide support at the adopted molybdena loadings (0.2 and 0.

A study of thoria on the surface of gamma-Al2O3.

The dispersion of thoria on the surface of gamma-Al2O3 and the surface properties of ThO2/gamma-Al2O3 samples, as well as the influence of the loading amount of thoria on the reduction behavior of copper oxide species, have been studied using XRD, XPS, FTIR, and TPR. The results indicate that the dispersion capacity of thoria, like that of ceria, is much lower than for two other tetravalent metal oxides, zirconia and titania, and the surface adsorption amount of the carbonyl compound and H2O slightly increases with increasing thoria loading. The different thoria loadings can influence the reduction behavior of the dispersed copper oxide by comparing the TPR results of CuO/ThO2/gamma-Al2O3 samples.