Genetic diversity and phylogeography of the endemic species Prunus mira in the Qinghai-Tibetan Plateau.

Studying the evolutionary history of plants in the Qinghai-Tibetan Plateau region provides a theoretical basis for the conservation and use of plant genetic resources. In this study, we analyzed five chloroplast gene fragments to examine the genetic diversity and phylogeography of Prunus mira in 577 individuals from 32 populations. The results indicated that P.

Saline-alkali stress affects the accumulation of proanthocyanidins and sesquiterpenoids via the MYB5-ANR/TPS31 cascades in the rose petals.

Rose () petals are rich in diverse secondary metabolites, which have important physiological functions as well as great economic values. Currently, it remains unclear how saline and/or alkaline stress(es) influence the accumulation of secondary metabolites in rose. In this study, we analyzed the transcriptome and metabolite profiles of rose petals under aline-alkali stress and uncovered the induction mechanism underlying major metabolites.

Insight into the Dynamic Nature of the Pt-CeO Interface in Dry Reforming of Methane.

Single-atom catalysts (SACs) are attractive in one-carbon (C1) chemistry because of their high atom efficiency. However, it is a great challenge for understanding the dynamic roles of SACs under operating conditions. Here, isolated Pt atoms trapped on defective CeO surface are investigated by experiments, especially operando techniques, which offers basic understanding of the nature and dynamic evolution of the Pt-CeO interface in dry reforming of methane (DRM).

Construction of biodegradable superhydrophilic/underwater superoleophobic materials with CNF (cellulose nanofiber) fence-like attached on the surface for efficient oil/water emulsion separation.

Superhydrophilic/underwater superoleophobic materials for the separation of oil-water emulsions by filtration have received much attention in order to solve the pollution problem of oil-water emulsion. In this paper, a fence-like structure on the surface of CNF/KGM (Konjac Glucomannan) materials by a simple method using CNF instead of metal nanowires was successfully developed based on the hydrogen bonding of KGM and CNF. The resulted organic CNF/KGM materials surface has outstanding superhydrophilic (WCA = 0°) in air and superoleophobicity (OCA≥151°) in water, which could separate oil-water mixtures with high separation efficiency above 99.

Enrofloxacin Rapid Detection in Aquatic Foods: Based on DNA Aptamer Sensor.

Enrofloxacin (ENR) is widely used as a synthetic fluoroquinolone antibiotic for disease control in aquatic animals. ENR aptamers were screened in this study using the magnetic bead-SELEX method, and a graphene oxide fluorescent sensor was developed to detect the ENR residues in aquatic products. Firstly, ENR was conjugated to amino magnetic beads by amidation reaction, and then the aptamer sequences showing high affinity to ENR were screened step by step by using the SELEX screening method.

Preparation of Sweet Potato Porous Starch by Marine Dextranase and Its Adsorption Characteristics.

Dextranase (EC 3.2.1.

Chloroplast genome data of five species: Clarifying genome structure and phylogenetic relationships.

species have considerable ecological and economic value, however, the phylogenetic relationships among remain controversy. In this study, we sequenced and assembled the chloroplast (cp) genomes of five species: , and . We then conducted comparative genomic analyses and constructed their phylogenetic relationships.

Isolation and Characterization of Bacteriophage VA5 against .

Bacteriophages, or phages, can be used as natural biological control agents to eliminate pathogenic bacteria during aquatic product cultivation. Samples were collected from seafood aquaculture water and aquaculture environmental sewage, and phage VA5 was isolated using the double-layer agar plate method, with as the host bacteria. The purified phage strain was subjected to genome sequencing analysis and morphological observation.

Nitric oxide synthase and its function in animal reproduction: an update.

Nitric oxide (NO), a free radical labile gas, is involved in the regulation of various biological functions and physiological processes during animal reproduction. Recently, increasing evidence suggests that the biological role and chemical fate of NO is dependent on dynamic regulation of its biosynthetic enzyme, three distinct nitric oxide synthase (NOS) according to their structure, location and function. The impact of NOS isoforms on reproductive functions need to be timely elucidated.

Sequencing and Phylogenetic Analysis of the Chloroplast Genome of Three Apricot Species.

The production and quality of apricots in China is currently limited by the availability of germplasm resource characterizations, including identification at the species and cultivar level. To help address this issue, the complete chloroplast genomes of L., L.

The prevalence, genetic diversity and evolutionary analysis of cachavirus firstly detected in northeastern China.

Canine cachavirus is a novel parvovirus belonging to the genus that was first detected in dogs in the United States. However, our knowledge of the prevalence and genetic characteristics of cachavirus is relatively limited. In this study, 325 canine fecal specimens collected from healthy and diarrheic dogs in northeastern China were screened with PCR.

Gastrodin synergistically increases migration of interleukin-13 receptor α2 chimeric antigen receptor T cell to the brain against glioblastoma multiforme: A preclinical study.

Therapy with chimeric antigen receptor T (CAR-T) cells involves using reformative T lymphocytes that have three domains, antigen recognition, transmembrane, and costimulating to achieve the therapeutic purpose. CAR-T therapy on malignant hematologic has been successful; however, its effectiveness in patients with solid tumors is still limited. Few studies exist confirming the efficacy of natural products on the function of CAR-T cells.

Mechanism underlying Müller cell pyroptosis and its role in the development of proliferative vitreoretinopathy.

Objectives: To explore the mechanism underlying Müller Cell Pyroptosis (MCP) and its role in the development of Proliferative Vitreoretinopathy (PVR).

Method: The expression of pyroptosis-related factors, namely, cysteinyl aspartate-specific proteinase (caspase-1), interleukin (IL)-1β, IL-18, and Gasdermin D (GSDMD), was detected by quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) and western blotting at the mRNA and protein levels, respectively, in retinal tissues. Müller and spontaneously Arising Retinal Pigment Epithelia (ARPE)-19 primary cells with GSDMD overexpression or knockdown were cultivated.

Genome-wide identification of auxin-responsive microRNAs in the poplar stem.

Background: Wood (secondary xylem) of forests is a material of great economic importance. Wood development is strictly controlled by both the phytohormone auxin and microRNAs (miRNAs). Currently, the regulatory mechanisms underlying wood formation by auxin-associated miRNAs remain unclear.

Optimized concurrent hearing and genetic screening in Beijing, China: A cross-sectional study.

Concurrent screening has been proven to provide a comprehensive approach for management of congenital deafness and prevention of ototoxicity. The SLC26A4 gene is associated with late-onset hearing loss and is of great clinical concern. For much earlier detection of newborns with deafness-causing mutations in the SLC26A4 gene, the Beijing Municipal Government launched a chip for optimized genetic screening of 15 variants of 4 genes causing deafness based on a chip to screen for 9 variants of 4 genes, and 6 variants of the SLC26A4 gene have now been added.

Human Motion Segmentation via Velocity-Sensitive Dual-Side Auto-Encoder.

Human motion segmentation (HMS) aims to segment a long human action video into a bunch of short and meaningful action clips. Existing supervised learning approaches need a large amount of training data which may be costly in real-world scenario, while most unsupervised clustering methods cannot fully explore the temporal correlations among human motions and hard to achieve promising performances. In our paper, we design a novel unsupervised framework, called Velocity-Sensitive Dual-Side Auto-Encoder (VSDA), for HMS task.

IL13Rα2-targeted third-generation CAR-T cells with CD28 transmembrane domain mediate the best anti-glioblastoma efficacy.

Chimeric antigen receptor (CAR)-modified T (CAR-T) cell therapy has been proven to be a powerful tool for the treatment of cancer, however, the limits are obvious, especially for solid tumors. Therefore, constantly optimizing the structure of CAR to improve its therapeutic effect is necessary. In this study, we generated three different third-generation CARs targeting IL13Rα2, with the same scFv, but different transmembrane domains (TMDs) from CD4, CD8 or CD28 (IL13-CD4TM-28.

Prevalence and genetic diversity of canine coronavirus in northeastern China during 2019-2021.

Canine coronavirus (CCoV) is associated with diarrhea in dogs, with a high incidence and sometimes even death. However, there is currently limited information about its prevalence and molecular characterization in northeastern China. Therefore, in this study, we examined 325 canine fecal specimens in four provinces in northeastern China from 2019 to 2021.