The change of break modulus drives human fibroblast differentiation in 3D collagen gels.

Extracellular matrix is one of the key environmental factors influencing cell survival and provides signals for cell morphological change, migration, proliferation and differentiation. However, the mechanism through which denatured collagen modulates the biological properties of fibroblasts, is unclear. We investigated the regulation of human fibroblast differentiation in vitro grown in collagen gels with different properties.

A severely infected diabetic foot treated successfully without using systemic antibiotics.

About 50% to 70% of all lower extremity amputations are related to diabetes infection. And antibiotic therapy is routinely used for all infected wounds to reduce the mortality of diabetic foot. Here, we report a case of diabetic foot with acute and deep severe infection.

[Advances in research of the mechanism of "covert disorder" in diabetic skin].

The diabetic ulceration is not uncommon, and becomes refractory, as the skin in a diabetic patient is relatively thin as well as hypoesthetic and less sensitive to temperature. As there are already preexisting histological and cellular derangement in the skin, healing of the skin injury is difficult, thus resulting in an intractable ulceration. When diabetes is not controlled, the skin contents of sugar and advanced glycation end product accumulate, invoking cellular deformation and accumulation of matrix metalloproteinases (MMP), resulting in an imbalance between MMP and its inhibitors, malfunction of growth factors, and inflammatory reaction.

[Exploring the three-dimensional structure of dermal tissues of normal skin and scar in rat with synchrotron radiation X-ray imaging technology].

Objective: To compare the morphological difference between dermal tissue of normal skin and that of scar in rat, and to explore its structural pattern.

Methods: The full-thickness skin and the scar tissue formed 3 weeks after wound healing from SD rats were harvested as samples, which were prepared appropriately afterwards. Samples were scanned and imaged with synchrotron radiation technology, micro-CT, and phase-contrast imaging technology.

[Exploratory study on the micro-remodeling of dermal tissue].

Objective: To explore the effect of three-dimensional structure of dermal matrix on biological behavior of fibroblasts (Fb) in the microcosmic perspective.

Methods: The three-dimensional structure of dermal tissue was analyzed by plane geometric and trigonometric function. Microdots structure array with cell adhesion effect was designed by computer-assisted design software according to the adhesive and non-adhesive components of dermal tissue.

[The effect of focal-adhesion micromanipulation in trauma and wound healing].

Objective: To explore the effect of focal-adhesion micromanipulation on the biological behavior of fibroblast.

Methods: Micro-pot was made by microcontact printing. The molecules of constitutive protein was adhered on micro-pot by self-assemble of peptides.

Antimitotic drug injections and radiotherapy: a review of the effectiveness of treatment for hypertrophic scars and keloids.

Scars are a common complication of surgery or burn wound management. Scars occur over the body, affecting people of both sexes and all ages. Scar therapy is a constant clinical challenge; antimitotic drugs and radiotherapy are used with varying degrees of success.

[Discoidin domain receptors (DDRs) expression in pathological scar fibroblast formation].

Objective: To explore the role of discoidin domain receptors (DDRs) in the formation of the keloid.

Methods: The real-time quantitative PCR was used to compare the DDRs expression in the keloids and normal fibroblasts.

Results: The level of DDR1 expression was significantly higher in keloid than in normal fibroblast (20.