[Artificial and bioartificial liver support systems for acute and acute-on-chronic liver failure: a meta-analysis].

Objective: To evaluate the effect of artificial and bioartificial liver support systems for management of acute and acute-on-chronic liver failure.

Methods: Articles documenting randomized clinical trials concerning any liver support systems vs standard conservative therapy, published between January, 1970 and June, 2008, were retrieved by database searching. Of the 1134 articles retrieved, 12 randomized trials involving 479 patients were included.

[Decellularization technology application in whole liver reconstruct biological scaffold].

Objective: To explore an innovative method for preparation of a whole-liver reconstruct scaffold with intact three-dimensional geometry, vasculature and bile duct by decellularization technology.

Methods: The portal vein was annulated and perfused sequentially with 1%Triton X-100 and 1%SDS for about 4 h, and then was perfused with phosphate buffered saline to dilute SDS residue. The retained structure was evaluated by histological analyses, including macroscopic, Hematoxylin-Eosin staining, Masson's trichrome staining, orcein staining and SEM.

[Preparation of whole-kidney acellular matrix in rats by perfusion].

Objective: To prepare rat whole-kidney acellular matrix (ACM) scaffolds using fluid perfusion method.

Methods: The kidneys with ureters and renal vessels were harvested from 12-week-old Wistar rats. Intravenous catheters were inserted through the renal arteries to establish channels for whole-kidney retrograde perfusion successively with heparinized PBS, 1% SDS, deionized water, 1% TritonX-100 and antibiotic-containing PBS under a pressure of 100 cmH2O.