Publications by authors named "Yu-xue Liao"
[Genetic evolution of VP1 of enterovirus type 71 in Shenzhen].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
June 2011
Article Synopsis
- - The study analyzed the genetic evolution of the VP1 protein in enterovirus type 71 (EV71) strains from Shenzhen, focusing on their nucleotide sequences and phylogenetic relationships.
- - A total of 35 strains showed a VP1 sequence homogeneity of 92.1%-100%, with significant variations when compared to subgenotypes A and B, indicating a shift in prevalent strains from cluster C4b to C4a over the years.
- - Yearly analysis revealed a gradual increase in sequence homogeneity from 2003 to 2008, alongside a specific mutation in the VP1 nucleotide at position 66 leading to an amino acid change, suggesting potential evolutionary adaptations of EV71.
[Detection and genotype analysis of sapovirus associated with sporadic diarrhea in Shenzhen in 2009].
Nan Fang Yi Ke Da Xue Xue Bao
June 2011
Article Synopsis
- - The study aimed to analyze the occurrence and genetic types of sapovirus (SaV) in cases of diarrhea in Shenzhen during 2009, involving a total of 852 fecal samples collected and tested using RT-PCR.
- - Out of the samples, 16 tested positive for SaV, yielding a positivity rate of 1.88%, with different genotypes identified, including SaV GI (3 GI.1 and 5 GI.2), GIV, and GII.
- - The findings indicate that SaV infections are present in Shenzhen, mainly with the GI genotype, and marks the first detection of GIV strains in China, highlighting genetic diversity in SaV infections across the country.
[Development of a SPA-ELISA method for detecting anti-coronavirus IgG antibodies in serum samples from fulvous fruit bats].
Nan Fang Yi Ke Da Xue Xue Bao
May 2008
Objective: To develop an simple and sensitive method for detecting anti-coronavirus IgG antibodies in bat sera based on enzyme-linked immunosorbent assay (ELISA).
Methods: A commercial ELISA kit for detecting SARS-CoV antibody was modified for detecting coronavirus antibodies in bat serum samples. The second antibody in the kit was replaced with horseradish peroxidase-conjugated protein-A (HRP-SPA) based on the characteristics of binding between Staphylococcus aureus protein A (SPA) and mammal IgG Fc fragment.