[Retracted] DNA methyltransferase 3b silencing affects locus‑specific DNA methylation and inhibits proliferation, migration and invasion in human hepatocellular carcinoma SMMC‑7721 and BEL‑7402 cells.

[This retracts the article DOI: 10.3892/ol.2015.

Carbon nanoparticle lymph node tracer improves the outcomes of surgical treatment in papillary thyroid cancer.

Background: Papillary thyroid carcinoma (PTC) is the most common thyroid malignancy which is generally accompanied by lymph node metastasis.

Objective: Our study evaluated whether carbon nanoparticle lymph node tracer can improve the outcomes of surgical treatment in papillary thyroid cancer (PTC).

Methods: Ninety-two patients were selected and underwent total thyroidectomy and lymph node resection.

LncRNA PTCSC3 affects drug resistance of anaplastic thyroid cancer through STAT3/INO80 pathway.

Background: LncRNA PTCSC3 is a tumor suppressor in thyroid cancer, and its role in drug resistance of anaplastic thyroid cancer (ATC) to chemotherapy drug doxorubicin was investigated in this study.

Methods: Expression of RNA and protein was analyzed by qRT-PCR and western blot, respectively. Flow cytometry was used to analyze the expression rate of CD133 cells.

DNA methyltransferase 3b silencing affects locus-specific DNA methylation and inhibits proliferation, migration and invasion in human hepatocellular carcinoma SMMC-7721 and BEL-7402 cells.

DNA methylation is an important regulator of gene transcription, and its role in carcinogenesis has been a topic of considerable interest in previous years. The present study examined the influence of DNA methyltransferase 3b (DNMT3b) on cell proliferation, migration and invasion, and the methylation status of identified tumor suppressor genes in hepatoma SMMC-7721 and BEL-7402 cells. DNMT3b was silenced by small interfering RNA (siRNA) in human hepatocellular carcinoma cell lines.

Identification of potential plasma biomarkers for esophageal squamous cell carcinoma by a proteomic method.

Among malignant tumors, the mortality rate of esophageal squamous cell carcinoma (ESCC) ranks sixth in the world. Late-stage diagnosis of ESCC increases the mortality. Therefore, more effective biomarkers for early diagnosis of ESCC are necessary.

[Application of surface-enhanced Raman spectroscopy to the determination of trace chemical hazards in food products].

In the present review, we summarized the research progress in applying SERS for the determination of illegal food additives, residual pesticides, banned or restricted antibiotics and other drugs. The nanosubstrates used in these studies included, but were not limited to, gold and silver nanosphere colloids, solid surface gold coated nanosubstrates, bimetallic nanosubstrates and spherical magnetic-core gold-shell nanoparticles, and etc. Standard solutions of a targeted chemical were normally tested first before analysis of relevant food in which the targeted chemical was commonly detected, and the tested food products included dairy products, condiments (such as chili powder and spices), fish, fruits and vegetables.

[Identification and expression of cytochrome C in tumor-bearing mice model with neuroblastoma].

Objective: To screen, identify and verify the serum specific protein of neuroblastoma in a tumor-bearing murine model and speculate about the source of cytochrome C.

Methods: NB cells (KP-N-NS) were inoculated subcutaneously into nude mice. And the sera samples of tumor-bearing mice (observation group, n = 14) and controls (control group, n = 25) were collected at 4 weeks to screen for and identify differentially expressed proteins.

[Relationship between mRNA expression and promoter methylation status of p73 gene in peripheral blood among children with Wilms' tumor].

Objective: To investigate the mRNA expression and promoter methylation status of p73 gene in the peripheral blood of children with Wilms' tumor (WT), and their relationship.

Methods: Forty-five children with WT were selected as the case group, and 15 sex- and age- matched children (without malignancies) who visited the hospital for physical examination or other reasons were selected as the control group. Peripheral blood was collected from both groups.

[Qualitative and quantitative detection of minced pork quality by near infrared reflectance spectroscopy].

The present study is concerning qualitative and quantitative detection of minced pork quality based on FT-near infrared (FT-NIR) spectroscopy and achieving the rapid approach to detecting the minced pork quality. Firstly, FT-NIR spectroscopy combined with partial least squares (PLS) and least squares-support vector machine (LS-SVM) was used for minced pork quality prediction including discrimination of the different muscle type of pig and quantitative detection of the fat, protein and moisture content of pork. The result indicated that 100% recognition ratio for calibration and 96% recognition ratio for validation were achieved by PLSDA for 4 different muscle types of pig.

Expression of vascular endothelial growth factor (VEGF) and VEGF-C in serum and tissue of Wilms tumor.

Background: Angiogenesis and lymphogenesis which were promoted by vascular endothelial growth factor (VEGF) and VEGF-C are important in the growth and metastasis of solid tumors. The high level of VEGF and VEGF-C were distributed in numerous types of cancers, but their distribution and expression in Wilms tumor, the most common pediatric tumor of the kidney, was unclear.

Methods: To learn about the distribution, mass spectroscopy and immunohistochemistry were used to measure the level of VEGF and VEGF-C in serum and tissue of Wilms tumor.

[Prediction of minced pork quality attributes using visible and near infrared reflectance spectroscopy].

The objective of the present study was to estimate minced pork meat quality using visible and near infrared (Vis-NIR) spectroscopy. Two hundred twenty five carcasses samples from longissimus dorsi muscle were scanned over the Vis-NIR spectral range from 350 to 1 015 nm and analysed for intramuscular fat (IMF), protein and moisture according to the official methods. Wavelet transform was employed to eliminate the spectra noise.

On-line prediction of fresh pork quality using visible/near-infrared reflectance spectroscopy.

Visible/near-infrared (Vis/NIR) spectroscopy was tested to predict the quality attributes of fresh pork (content of intramuscular fat, protein and water, pH and shear force value) on-line. Vis/NIR spectra (350-1100 nm) were obtained from 211 samples using a prototype. Partial least-squares regression (PLSR) models were developed by external validation with wavelet de-noising and several pre-processing methods.

[Online determination of pH in fresh pork by visible/near-infrared spectroscopy].

The present research was focused on determination of the pH value online by visible and near-infrared spectroscopy. In the part of data gathering, fresh pork longissimus dorsi was moving at the constant velocity of 0.25 m x s(-1) on the conveyor belt, and the visible and near-infrared diffuse reflectance spectrum (350-1 000 nm) was captured.

[Qualitative analysis of fragrant pear class based on near infrared diffuse reflectance spectroscopy].

A method was developed to automatically discriminate the persistent calyx fruit and fruit without calyx of fragrant pear by means of near infrared spectroscopy (NIRS). The prediction performance of different band regions range, different principal component numbers and different preprocessing methods of the spectra (multiplicative signal correction, standard normal variate, and derivative spectra) together with discriminant analysis (DA) was also investigated, and The calibration model was established to classify the different kinds of fragrant pear. The research results for the fragrant pear classification showed that DA calibration models using these parameters with band regions between 9 091 and 4 000 cm(-1) and original spectra are optimal, with the percentage of correct sample classification being 100% and 95% for the calibration and validation set, respectively.

[Detection and identification of specific serum biomarkers in papillary thyroid cancer].

Objective: To detect and identify the potential specific serum biomarkers for diagnosis of papillary thyroid cancer.

Methods: Samples of 35 patients with papillary thyroid carcinoma, 40 patients with benign thyroid nodule and 34 healthy individuals were analyzed using the SELDI-TOF ProteinChip System and bioinfomation technology to find the differential peaks which were separated by HPLC and then further analyzed by LC-MS/MS. The protein sequences were analyzed by SEQUEST software and searched in Bioworks database.

[Screening and identification analysis of serum protein biomarkers in nephroblastoma].

Objective: To screen and characterize the serum protein biomarkers in nephroblastoma so as to establish the proteins as the specific serum biomarkers for diagnosis and prognosis monitoring.

Methods: The differential protein peaks were located by detecting serum samples of preoperative and postoperative patients and normal children using the SELDI-TOF MS technology. After purification, the differential proteins were further analyzed by LC-MS/MS and the protein sequences searched in database.