[Diagnosis of Muscular Dystrophy Using Western Blot with Micro-sample of Muscle].

Objective: To diagnose muscular dystrophy using Western blot (WB) by improving the method of the protein extraction.

Method: Firstly,we compared the effect of different sample buffer solutions and processing Methods on the extraction of muscle protein in rats,then selected the appropriate extracting method and the process of the muscular protein.

Results: We put the selected sample buffer into the micro-sample,then mixed.

[The clinical and muscular pathological study of dermatomyositis with perifascicular atrophy changes].

Objective: To investigate the clinical and pathological characteristics of dermatomyositis with muscular perifascicular atrophy (PFA).

Methods: A series of 104 consecutive patients clinically and pathologically diagnosed as dermatomyositis by muscle biopsy in our laboratory from December, 2003 to August, 2011, were enrolled in this study. Muscle biopsy of all the enrolled patients had shown PFA of muscle fibers.

[Clinical and pathological features of glycogen storage disease type III].

Objectives: To summarize the clinical and pathological features of glycogen storage disease (GSD) type III.

Methods: The clinical data of 12 GSD type III, 8 males and 4 females, aged 2 - 27, were collected. The biopsy specimens of quadriceps muscle of thigh underwent HE and histochemical staining and light and electron microscopy.

Evaluation of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes with magnetic resonance imaging and proton magnetic resonance spectroscopy.

Objective: To study the characteristics of spectra on proton magnetic resonance spectroscopy (1H-MRS) and its value in patients with mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS).

Methods: Seven clinically diagnosed patients with MELAS underwent magnetic resonance imaging (MRI) and 1H-MRS examinations. The 1H-MRS techniques, characteristics of the spectra, and its correlation with the laboratory tests were analyzed.

[Primary agiitis of central nervous system].

The common clinical manifestations of the primary agiitis of the central nervous system include burst of headache, dementia, change of aptitude, paralysis of cranial nerves, and recurrent focal depletion of the neural function. Lptomeningeal and brain biopsy are still the gold criteria for diagnosis. The prognosis may be improved after cortin and immunosuppressant therapy.

[Cloning, expression and purification of neural specific HuD cDNA].

Objective: To prokaryoticly express and purify HuD protein and its RNA recognition motifs.

Methods: HuD protein was prokaryoticly expressed and purified by molecular cloning technology. Its biologic activity was testified by Western Blot.