[Effect of different nanophase hydroxyapatite particles on osteoblasts metabolism].

Purpose: To compare two different structural nanophase hydroxyapatite(HA) particles on the proliferation activity and functions of osteoblasts.

Methods: Primary culture of osteoblasts from rat calvaria was established and then cultured on the coatings of different size of nano particles (groupI 20-40nm,group II 70-100nm), the HA coatings without nano-particles was used as control group. Proliferation activity, protein content and synthesis of alkaline phosphatase(ALP) by osteoblasts were examined by MTT assay, coomassic brilliant blue method and PNPP test, and statistical significance was assessed by multiple comparison (q test, SNK) in one-way analysis of variance (ANOVA) with SPSS10.