[Studies on the chemical constituents of Ficus microcarpa].

Objective: To study the chemical constituents of the Ficus microcarpa.

Methods: Isolation and identification were carried out by using various chromatography techniques and spectral methods.

Results: Eight compounds were isolated.

Four new minor taxanes from cell cultures of Taxus chinensis.

Four new minor taxanes (1-4) have been isolated from Ts-19 cell cultures of Taxus chinensis together with five known taxanes (5-9) by silica gel chromatography combined with semi-preparative HPLC chromatography. On the basis of the analyses of the chemical and spectroscopic (IR, MS, 1D, and 2D NMR) data, the structures of new compounds were elucidated as 5alpha-hydroxy-2alpha,10beta-diacetoxy-14beta-(3-hydroxy-2-methyl-butyryl)oxytaxa-4(20),11-diene (1), 2alpha,5alpha,10beta-triacetoxy-14beta-(2-hydroxy-propionyl)oxytaxa-4(20),11-diene (2), 2alpha,5alpha,10beta-triacetoxy-14beta-(2-hydroxy-3-methyl-butyryl)oxytaxa-4(20),11-diene (3), and 2alpha-benzoxy-4alpha,9alpha,10beta,13alpha-tetraacetoxytax-11-ene (4), respectively. Compounds 1-5 were pharmacologically evaluated for their cytotoxicities against five human cancer cell lines (HCT-8, Bel-7402, BGC-823, A549, and A2780) and their reversing activity towards multi-drug resistance A549/taxol tumor cell line, and the results showed that all of the tested compounds exhibited very low cytotoxicities, while compound 4 possessed twice the reversing activity as that of verapamil at 10 muM.

[The comparative study on morphology and polysaccharides content of Dendrobium loddigesii from different habits].

The morphological characters and the polysaccharides content of Dendrobiam loddigesii from different habits were studied in this paper. The results showed that there were remarkable differences in morphology from five areas, such as stem height and diameter, laminae sizes and figures, amount of lateral bud, and texture of stem. The content of polysaccharides from sample of Xingyi and Anlong, Guizhou Province, respectively 22.

[ITS sequence identification of Radix Bupleuri].

Objective: To provide the basis of molecular authentication of Radix Bupleuri by the comparison of the internal transcribed spacer (ITS) sequences of five kinds of Radix Bupleuri in common use.

Method: Firstly, total DNA of five kinds of Radix Bupleuri was extracted. Secondly, the ITS sequence was amplified by PCR with universal primer of ITS and PCR product was directly sequenced after purification.

[Studies on the botanical sources and DNA molecular identification of Herba Asari based on ITS sequence].

Objective: To explore the genetic basis of using three species of Asarum as Herba Asari to determine the taxonomic positions of Asarum heterotropoides and A. siebodii; and to apply DNA molecular analysis as a tool for identification of Herba Asari.

Method: PCR, purification, sequence analysis were prerformed.

[Identification of Pterocephalus hookeri].

Objective: To study the identification method of Pterocephalus hookeri.

Method: The microscopical, Physicochemical and TLC methods were used.

Result And Conclusion: The convenient and effective identification methods for P.

[Study on the genetiey relationship between Chinese and Korean medicinal materials of niuxi with the method of RAPD].

Objective: To study the hereditary relationship between Chinese and Korean medicinal materials of Niuxi achyranthis root.

Method: Ten samples of four kinds of Niuxi was studied with the method of RAPD.

Result: Expanded product showed total record of 100 spectrum bands, which proved that the hereditary gap between Korean self-produced A.

[Studies on chemical constituents of Cuscuta chinensis].

Objective: To study the chemical constituents of the seeds of Cuscuta chinensis.

Method: The separation was carried out by polyamide and silica gel chromatography, and the compounds were identified by means of physico-chemical and spectroscopic methods.

Results: Eight compounds were isolated from the plant and identified as quercetin 3-O-beta-D-galactoside-7-O-beta-D-glucoside (I), quercetin 3-O-beta-D-apiofuranosyl-(1-->2)-beta-D-galactoside (II), hyperoside (III), isorhamnetin (IV), kaempferol (V), quercetin (VI), d-sesamin (VII) and 9(R)-hydroxy-d-sesamin (VIII).

[The influences of inorganic elements in soil on the geolism of Atractylodes lancea].

Objective: To study the influence of inorganic elements in soil on the geolism of Atractylodes lancea.

Method: The concentrations of 15 kinds of inorganic elements of regional A. lancea and their correspondingly soils were determined, and the data were analysed with the SPSS10.

[A study on genetic variation between wild and cultivated populations of Paeonia lactiflora Pall].

Aim: To discuss the genetic differentiation between wild and cultivated populations of Paeonia lactiflora Pall., and to find the reason for forming the genuineness of Radix Paeoniae rubra (Chishao) and Radix Paeoniae alba (Baishao).

Methods: Forty three representative samples of P.