[Analysis of classical prescription Jinshui Liujun Jian based on ancient literature].

To provide the ancient literary evidence support for the clinical application and development of classical prescription based on systematical collection and analysis of the ancient Chinese medical literature containing Jinshui Liujun Jian, including its origin and development. Bibliometric analysis was used and information of Jinshui Liujun Jian in ancient Chinese medical literature was then collected for statistical analysis of formula compositions, main indications, dosage, preparation methods, etc. A total of 151 valid items of data were obtained from 48 ancient Chinese medicine books.

[Secretion expression of Mt. heat shock protein 70 in Pichia pastoris and identification of the protein].

To obtain the expression of Mycobacterium tuberculosis heat shock protein 70 in methylotropic yeast. The expression vector pPIC9K-hsp70 was constructed, linearized and introduced into Pichia pastoris GS115 by electroporation. The result protein was secreted into the supernatant induced by 0.

[Antivirus effect of plasmid co expressing hepatitis B surface antigen and granulocyte macrophage-colony stimulating factor].

Objective: To investigate whether GM-CSF can enhance the antiviral effect of HBsAg DNA vaccine.

Methods: Divided animals into 8 groups. Group A: pcDNA3.

Relationship between the different replication status of HBV and mutations in the core promoter in mothers and their children infected via mother-to-infant transmission.

Objective: To study the relationship between the different replication status of hepatitis B virus (HBV) and mutations in the core promoter (CP) in mother and her child infected by mother-to-infant transmission.

Methods: The core promoter was amplified by PCR and cloned into pGEM-T vector with the T-A cloning technique. The recombinant plasmid pGEM-CP was confirmed by digestion with restriction enzyme Apa I and Sac I.

[Establishment of consensus sequence of PreS/S of hepatitis B virus with genotype B/serotype adw2 or genotype C/serotype adrq+ prevailing in Chongqing of China].

Objective: To develop consensus sequence of HBV PreS/S with different subtype in Chongqing of China.

Methods: The gene of PreS/S of HBV in 18 AsC was sequenced. The genotype and serotype of HBV were determined.

[Specific immune responses in Balb/c mice induced by plasmid coexpressing hepatitis B surface antigen and granulocyte-macrophage colony stimulating factor].

Objectives: To investigate the improvement of specific immune responses induced by plasmid coexpressing hepatitis B surface antigen (HBsAg) and granulocyte-macrophage colony stimulating factor (GM-CSF).

Methods: All Balb/c (H-2d) mice were immunized with pGM-CSF/S, pS/GM-CSF, pS or control plasmids. 4 weeks later, anti-HBs titer and the levels of IL-2, IL-4 and IFN-gamma in the supernatant of splenocytes were detected using enzyme- linked immunosorbent assay (ELISA), and HBsAg-specific cytotoxic T lymphocytes (CTL) activity was measured with a 51Cr release assay, using P815/S transfectants as target cells.

[A study of the characteristics of pre S/S gene mutation of hepatitis B virus (HBV) in mothers and HBV-infected children via mother-to-infant transmission].

Objective: To investigate the characteristics of mutations in pre S/S gene of HBV in asymptomatic carrier (AsC) children infected through mother-to-infant transmission and their AsC mothers with different degree of viremia.

Methods: According to the levels of viremia in every pair of mother and child, 15 pairs of child and mother were divided into three groups 5 pairs in each group in this study: group I (both children and mothers had high viremia), group II (children had high and mothers had low viremia) and group III (children had low and mothers had high viremia). pre S/S gene was amplified by PCR and cloned into pGEM-T vector with T-A cloning technique.

[PreS/S gene mutations in HBV in children infected through mother-to-infant transmission and in their mothers].

Objectives: To investigate the characteristics of mutations in PreS/S gene of HBV in children infected through mother-to-infant transmission and in their mothers with different degree of viremia.

Methods: There were 15 pairs of child and mother in this study. Mothers of all children were chronic asymptomatic HBsAg carrier (ASC) before pregnancy and the children were not inoculated against HBV after birth.

[Prokaryocytic expression of the shortened hepatitis B surface antigen and antigenic analysis].

Objective: To study the expression of the shortened hepatitis B surface antigen in prokaryocyte and detect the antigenic characters.

Methods: Firstly, the gene fragments coding the 152 and 124 amino acids of the carboxyl terminus of hepatitis B surface antigen (HBsAg) were amplified by polymerase chain reaction (PCR). Secondly, they were cloned to plasmid pBKS+, and the accuracy of those constructions were confirmed by restriction enzyme digestion and DNA sequencing.