[Preparation of agar-paraffin double-embedded longitudinal sections of Schistosoma japonicum].

Schistosoma japonicum adults are pre-embedded in a double-layer agar and made the block, then dehydrated with alcohol, isobutyl alcohol and n-butyl alcohol. Various staining procedures can be conducted after conventional sectioning and dewaxing. Complete longitudinal serial sections of the pre-embedded worms can be obtained, and the desired sections can be easily located accurately.

[Induction of protective immunity against Schistosoma japonicum by pcDNA3.1/Sj20.8 nucleic acid vaccine in BALB/c mice].

Sj20.8 gene was amplified by PCR and inserted into eukaryotic expression plasmid pcDNA3.1 to construct recombinant plasmid pcDNA3.

[Acquisition and analysis of 149 ESTs and 18 novel genes of Schistosoma japonicum].

Objective: To acquire and analyze adult stage Schistosoma japonicum (Chinese strain) expressed sequence tags and new genes from an adult S. japonicum cDNA library, and to search new vaccine candidates and drug targets.

Methods: A cDNA library was constructed from adult stage S.

[Amplification and sequencing of the gene coding for mucin-like protein from Schistosoma japonicum].

Objective: To amplify and sequence the partial gene coding for mucin-like protein from Chinese isolates of Schistosoma japonicum (SjMLP).

Methods: The antigenic determinants of SmMLP were predicted by PCGENE software and specific oligonucleotide primers were designed and synthesized. Total RNA was isolated from adult worms of S.

[Cloning, sequencing and expressing of microneme protein 1 partial gene in toxoplasma gondii ZS2 isolate].

Objective: To construct a recombinant prokaryotic expression vector (plasmid) containing microneme protein 1 (MIC1) partial gene in toxoplasma gondii (T. gondii) ZS2 isolate. The gene was expressed in varied Escherichia coli (E.