Extraction of tree crown parameters of high-density pure plantations by combining the U-Net model and watershed algorithm.

As one of the important timber species in China, is widely distributed in southern China. The information of tree individuals and crown plays an important role in accurately monitoring forest resources. Therefore, it is particularly significant to accurately grasp such information of individual tree.

Comparison of chimeric antigen receptor-T cell-mediated cytotoxicity assays with suspension tumor cells using plate-based image cytometry method.

In the recent decade, chimeric antigen receptor (CAR)-T cell therapy has revolutionized strategies for cancer treatments due to its highly effective clinical efficacy and response for B cell malignancies. The success of CAR-T cell therapy has stimulated the increase in the research and development of various CAR constructs to target different tumor types. Therefore, a robust and efficient in vitro potency assay is needed to quickly identify potential CAR gene design from a library of construct candidates.

[Comparison of artificial neural network with compatible biomass model for predicting aboveground biomass of individual tree].

Forest biomass is an important index in forest development planning and forest resource monitoring. In order to provide a more efficient and low-biased method for estimating individual tree biomass, we introduced artificial neural network here. We used the data of aboveground biomass of 101 trees harvested from the Dongzhelenghe Forest Farm in Heilongjiang Province to develop four aggregation model systems (AMS), based on different combination of the variables (diameter at breast height, tree height, crown width).

New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density.

Platelet-rich plasma (PRP) can stimulate the proliferation of stem cells and have a positive effect on tissue repair. Although many commercialized PRP preparation kits are already on the market, first-line clinical workers are still not satisfied with most of the PRP kits. The work of commercial PRP kits is based on the density of blood elements.

The preparation of cell-containing microbubble scaffolds to mimic alveoli structure as a 3D drug-screening system for lung cancer.

Cancer is the leading cause of mortality worldwide, and lung cancer is the most malignant. However, the high failure rate in oncology drug development from in vitro studies to in vivo preclinical models indicates that the modern methods of evaluating drug efficacies in vitro are not reliable. Traditional 2D cell culture has proved inadequate to mimic real physiological conditions.

[Responses of radial growth to climate change in Pinus massoniana at different altitudes and slopes.].

With dendrochronology method, standard and residual chronologies of Pinus massoniana were established at low altitude (260 m), middle altitude (460 m), high altitude (690 m), sunny slope (270 m), and shady slope (265 m). Relationships between the tree-ring width and the climatic factors were quantified using correlation analysis and redundancy analysis (RDA). The optimal multiple regression models for the radial growth of P.

[Spatial variation of carbon density in Pinus massoniana forest in Jiangxi Province, China].

Carbon density is one of the important indicators for carbon sequestration capacity in forest ecosystems. The analysis of spatial variation in forest carbon density can provide scientific basis for management of forest carbon sink. We investigated the carbon density of Pinus massoniana forest in Jiangxi Province.

Comparison of object-oriented remote sensing image classification based on different decision trees in forest area.

Geographic Object-Based Image Analysis (GEOBIA) was a product under the background of increasing high-resolution remote sensing data. How to improve the accuracy and efficiency of classification of high-resolution images is one of the important topics in image processing. After objects segmented multiscale by QuickBird image was classified, the efficiency of C5.

Protective effects of aucubin on osteoarthritic chondrocyte model induced by hydrogen peroxide and mechanical stimulus.

Background: During the onset of osteoarthritis (OA), certain biochemical events have been shown to accelerate cartilage degradation, including the dysregulation of cartilage ECM anabolism, abnormal generation of reactive oxygen species (ROS) and overproduction of proteolytic enzymes and inflammatory cytokines. The potency of aucubin in protecting cellular components against oxidative stress, inflammation and apoptosis effects are well documented, which makes it a potential candidate for OA treatment. In this study, we aimed to evaluate the protective benefits of aucubin against OA using HO and compression induced OA-like chondrocyte models.

C-phycocyanin alleviates osteoarthritic injury in chondrocytes stimulated with HO and compressive stress.

During the progression of osteoarthritis (OA), dysregulation of extracellular matrix anabolism, abnormal generation of reactive oxygen species (ROS) and inflammatory cytokines have been shown to accelerate the degradation process of cartilage. The potency of c-phycocyanin (C-PC) to protect cellular components against oxidative stress, along with its anti-inflammation and anti-apoptosis effects, are well documented; however, effects of C-PC on OA are still unclear. In this study, we aimed to investigate the effects of C-PC on OA using HO or compression-stimulated OA-like porcine chondrocyte models.

Development of gelatin nanoparticles conjugated with phytohemagglutinin erythroagglutinating loaded with gemcitabine for inducing apoptosis in non-small cell lung cancer cells.

Gelatin is an efficient drug delivery vehicle for attaching targeting molecules like phytohemagglutinin erythroagglutinating (PHA-E) and carrying the chemotherapeutic agent gemcitabine (GEM). Fluorescent gelatin nanoparticles (GNPs) conjugated with PHA-E and carrying gemcitabine (GNP-(PHA-E)-GEM) were synthesized by nanoprecipitation for guiding gemcitabine-loaded gelatin nanoparticles to NSCLC by PHA-E targeting. GNPs have a uniform narrow size distribution and spherical shape, and their particle size is about 290 nm.

Correction: quantitative analysis of ligand-EGFR interactions: a platform for screening targeting molecules.

[This corrects the article DOI: 10.1371/journal.pone.

Quantitative analysis of ligand-EGFR interactions: a platform for screening targeting molecules.

Epidermal growth factor receptor (EGFR) is often constitutively stimulated in many cancers owing to the binding of ligands such as epidermal growth factor (EGF). Therefore, it is necessary to investigate the interaction between EGFR and its targeting biomolecules. The main aim of this study was to estimate the binding affinity and adhesion force of two targeting molecules, anti-EGFR monoclonal antibody (mAb LA1) and the peptide GE11 (YHWYGYTPQNVI), with respect to EGFR and to compare these values with those obtained for the ligand, EGF.

Fabrication of uniform 4H-SiC mesopores by pulsed electrochemical etching.

In this letter, the uniform 4H silicon carbide (SiC) mesopores was fabricated by pulsed electrochemical etching method. The length of the mesopores is about 19 μm with a diameter of about 19 nm. The introduction of pause time (T off) is crucial to form the uniform 4H-SiC mesopores.

[Simulation of above ground biomass in Larix olgensis plantation].

By the method of harvesting sampling trees, the information of aboveground biomass in 34 plots of a Larix olgensis plantation were collected, of which, the information from 29 plots was selected and fitted with stand factors and TM image RS factors, respectively to establish biomass models, and the information from the rest 5 plots was used to verify the models accuracy. The aboveground biomass in the Larix olgensis plantation could be linearly fitted with either stand factors or RS factors. For the young-middle aged trees, the estimation accuracy of stand factors model was higher (P(stand) = 94.

A controlled release system of superoxide dismutase by electrospun fiber and its antioxidant activity in vitro.

In this paper, a new controlled release system of superoxide dismutase was developed by electrospun composite fibers. Highly loading efficacy of sod from 85.6 to 98.

[Purification and analysis of Cimicifuga foetida glycoprotein ( CF- I )].

A kind of glycoprotein ( CF- I ) was extracted from Cimicifuga foetida and purified by DEAE-Cellulose ( DEAE-52) and Sepharose CL-4B. It was identified to be homogeneous glycoprotein complex by electrophoresis and fast protein liquid chromatography (FPLC). alpha-glucosidic bond was detrermined by IR.