[Umbilical Cord-derived Mesenchymal Stem Cells Grafts for Neonatal Rats Model of HIBD: the Mechanism of PI3K/Akt Signaling Pathway].

Objective: To investigate the role of phosphatidylinositol 3 kinase/protein kinase B (PI3K/Akt) signaling pathway for umbilical cord-derived mesenchymal stem cells (UC-MSCs) treating in neonatal rats with hypoxia-ischemia brain damage (HIBD).

Methods: P10 SD rats were divided into sham group, MSCs group, inhibitor (LY 294002) group (LY group) and HIBD group randomly. To establish a neonatal rat model of HIBD, UC-MSCs labeled with Brd U were transplanted into the lateral ventricle of rats.

[Effect of Berberine on the Insulin Resistance and TLR4/IKKbeta/NF-kappaB Signaling Pathways in Skeletal Muscle of Obese Rats with Insulin Resistance].

Objective: To explore the possible mechanism of berberine improve insulin resistance in skeletal muscle of obese rats.

Methods: Forty Wistar rats were divided into normal control group (NC group, n =10) and high fat food group (HF group, n=30). After the obese model rats established successfully, the rats of NC group (n=10) and HF group (n=10) were sacrificed.

[The risk factors of ventilator-associated pneumonia in newborn and the changes of isolated pathogens].

Objective: To study the risk factors of ventilator-associated pneumonia (VAP) in newborn and the profiles of isolated pathogens.

Methods: The clinical data of 179 neonates in the previous group (from December 2005 to December 2008) and 331 neonates in the present group (from January 2009 to January 2012) admitted into the neonatal intensive care unit (NICU) and received mechanical ventilation for equal or longer than 48 hours were respectively reviewed and analyzed,and their isolated pathogen profile and drug sensitivity were also compared in two groups.

Results: The incidence of VAP in the previous group and the present group were 22.

Epidemiological survey on newborns born at the obstetric departments in hospitals in mid-southern region of China in 2005.

Objective: To investigate the situations at birth of newborns in the mid-southern region of China by performing a survey on the newborns born at urban hospitals.

Methods: A total of 23 hospitals in the mid-southern region of China were selected to participate in this survey. The data of 15,582 newborns who were born at the obstetric departments from January 1, 2005 to December 31, 2005 were retrospectively investigated.

Incidence of brain injuries in premature infants with gestational age ≤ 34 weeks in ten urban hospitals in China.

Background: There is a large number (1.5 million per year) of premature births in China. It is necessary to obtain the authentic incidences of intraventricular hemorrhage (IVH) and periventricular leukomalacia (PVL), the common brain injuries, in Chinese premature infants.

Epidemiologic survey on hospitalized neonates in China.

Objective: To carry out a nationwide epidemiologic survey on the neonates in urban hospitals with an attempt to understand the disease spectrum and treatment outcomes of hospitalized neonates in China.

Methods: The clinical data of 43,289 hospitalized neonates from 86 hospitals in 47 Chinese cities (22 provinces) between January 1, 2005 and December 31, 2005 were retrospectively analyzed.

Results: The male:female ratio was 1.

[A survey of neonatal births in maternity departments in urban China in 2005].

Objective: To study the epidemiology of births in urban China.

Methods: A retrospective study was conducted on neonates born in 2005 in the maternity departments of 72 urban hospitals from 22 provinces in China.

Results: A total of 45722 infants born between January 1, 2005 and December 31, 2005 were enrolled.

[An epidemiologic investigation of newborns from obstetric departments in the central south region of China].

Objective: To investigate the birth information of newborn infants from obstetric departments in the Central South Region of China.

Methods: A retrospective investigation was carried out in 15582 newborns from obstetric departments of 23 hospitals in the Central South Region of China between January 1 and December 31 of 2005.

Results: The sex ratio (male/female) of neonates was 1.

[Multicenter investigation for incidence of periventricular leukomalacia in premature infants in China].

Objective: Sponsored by the Subspecialty Group of Neonatology of Pediatric Society, China Medical Association, more than 10 large-scale hospitals participated in the near two-year multicenter investigation for brain injuries in premature infants in China. This study presented the investigation result for the incidence of periventricular leukomalacia (PVL) in premature infants from 10 Third Class A Level hospitals.

Methods: The premature infants with a gestation age<37 weeks in the 10 hospitals were given routine cranial ultrasound scanning within seven days after birth, and then repeated every 3-7 days until discharge from January 2005 to August 2006.

[Protective effect of calpain inhibitor-3 on hypoxic-ischemic brain damage of neonatal rats].

Objective: The mechanisms of hypoxic-ischemic brain damage (HIBD) are still largely unknown. Elevation of intracellular calcium concentration and subsequent calcium-dependent proteases activation such as calpains seem to play an important role in the process of neuronal death. Calpain inhibitors showed neuroprotective effects in adult rat cerebral ischemia models.

[Expression of F-actin and RhoA in experimental hypoxic-ischemic white matter damage in premature SD rats].

Objective: White matter damage (WMD) in preterm infants is a well-recognized serious complication of prematurity. The collapse of cell skeleton of growth cone after hypoxia-ischemia (HI) is considered as the basic neuropathologic change of the long-term residuals of premature white matter damage. F-actin is the major component of cell skeleton and maintains the normal form of cells, its function and potential mechanism of WMD have not been reported.

[Expression of chemokine receptor CCR2 in cerebral tissue of newborn rat with experimental hypoxic-ischemic brain damage].

Objective: To observe the expressions of mRNA and protein for the CCR2 in cerebral tissue with experimental hypoxic-ischemic brain damage (HIBD) to newborn rat.

Methods: The HIBD model of SD rat was set up by ligating the right carotid artery and exposing the animals to 8% oxygen for 2.5 h.

[The influence of oxygen and glucose deprivation on the expression of neurite growth inhibitor Nogo-A in oligodendrocyte precursor cell of rat].

Objective: To investigate the Nogo-A expression and its regeneration inhibition role in oligodendrocyte precursor cells (OLPs) after oxygen & glucose deprivation (OGD).

Methods: The OLPs were separated by the improved procedure of separation and purification through agitation and then cultured in chemically defined medium. The OGD model of OLPs was set up by using Na2S2O4 and Earle's fluid in the medium in vitro.

[Expressions of micro-calpain mRNA and protein in cerebral tissues of neonatal rats after hypoxic-ischemic brain damage].

Objective: To identify the expression of micro-calpain mRNA and protein in cerebral tissues of neonatal rats after hypoxic-ischemic brain damage (HIBD) and its role in the pathogenesis of HIBD.

Methods: Seven-day-old Sprague-Dawley rats were randomly divided into seven groups: control group and groups with 0, 6, 12, 24, 48, and 72 hours of exposure to HIDB. Quantitative real-time fluroscent polymerase chain reaction and Western blot technique were employed to detect the expression of micro-calpain mRNA and its protein respectively.

[NgR expression in oligodendrocyte precursor cells and its changes after oxygen & glucose deprivation in neonatal rats].

Objective: This study examined the NgR expression in oligodendrocyte precursor cells (OLPs) and its changes after oxygen & glucose deprivation (OGD) in order to explore the role of NgR expression in the regeneration of OLPs after OGD in neonatal rats.

Methods: The OLPs from 2-day-old neonatal rats were separated by improved separation and purification through agitation and then cultured in chemically defined medium. OLPs OGD model was prepared using the medium consisting of Na2S2O4 and Earle's fluid in vitro.

[Expression of P75NTR protein and RhoA mRNA in the brain of neonatal rats with white matter damage].

Objective: Recent studies have indicated that the signal pathway of NgR-P75NTR- RhoA plays a key role in nerve injury and remodeling, but its exact mechanism and the role of the downstream molecule RhoA regulated by P75NTR remain unclear in hypoxia-ischemia (HI) neonatal animals. The present study was designed to assess the expression of P75NTR protein and RhoA mRNA in neonatal white matter and to investigate their relationship in newborn rats with white matter damage (WMD).

Methods: The rat WMD model was established by the ligation of right common carotid artery, followed by 6% hypoxia exposure for 4 hrs.

[Roles of glutamate receptor 2 and cellular free calcium in the pathogenesis of periventricular leukomalacia].

Objective: To study the expression of the AMPA receptor subunit glutamate receptor 2 (GluR2) and the cellular free calcium concentration in the white matter of neonatal rats with periventricular leukomalacia (PVL) and their roles in the pathogenesis of PVL.

Methods: A PVL model was prepared by unilateral carotid artery ligation (UCL) followed by exposure to 6% oxygen for 4 hrs in 2-day-old rats. The neonatal rats performed a sham operation, without hypoxia-ischemia (HI), were used as the control group.

[Expression of ngR mRNA and ngR protein in brain tissues of newborn rats with hypoxic ischemic brain damage].

Unlabelled: OBJECTIVE To study the expression of NgR mRNA and NgR protein in the brain tissues of newborn rats with hypoxic ischemic brain damage (HIBD) its implications.

Methods: Seventy two newborn SD rats (7 days) were randomly divided into two groups, with or without surgical induced HIBD. RT-PCR was used to detect the expression of NgR mRNA.

[Brain mitochondrial DNA damage of newborn piglets following hypoxia-ischemia].

Objective: This study investigated the 8003 base pair (bp) fragmentation damage of brain mitochondrial DNA in newborn piglets at different times after hypoxic-ischemic brain damage (HIBD) so as to explore the biomolecular foundation of neonatal neuronal metabolic disorders.

Methods: Fifty 3-day-old piglets were randomly assigned into Control and HIBD groups. The HIBD group was subdivided into groups sacrificed at 0, 24, 48 and 72 hrs post-HIBD (n=10).

[Alternations of ADF and G-actin in renal tubular epithelial cell of neonatal rats during kidney ischemic Injury].

Objective: To study the changes of ADF and G-actin in renal tubular epithelial cell of neonatal rats during kindney ischemic injury. To explore the interactions between ADF and G-actin and their relationships with actin cytoskeleton.

Methods: Twenty-four neonatal rats were used to the experiments due to have the weights from 30 to 40 g respectively.

[Alteration and its significance to expression of aquaporin-4 in cultured neonatal rat astrocytes in the model of hypoxic damage].

Objective: To investigate the expression of aquaorin-4 (AQP4) in the cultured neonatal rat primary astrocytes during hypoxia and during reoxygenation after hypoxia.

Methods: The astrocytes were randomly assigned to the hypoxia group (the astrocytes were exposed to hypoxia for 3 h, 6 h, 12 h and 24 h respectively), the reoxygenation group (after exposure to 12 h hypoxia, the astrocytes were returned to normoxic cultures for 3 h, 6 h, 12 h and 24 h respectively), and the normoxic group (control group). AQP4 mRNA expression in astrocytes of all groups was detected by Real Time PCR, and AQP4 protein expression was determined by immunofluorescence.

[Effect of hypoxia on energy metabolism of G1/S renal epithelial cells of piglets].

Objective: To determine whether effect of energy metabolism on G1/S renal tubular epithelial cells (RTEs) is strongly associated with anoxemia.

Methods: Neonatal pig's RTEs were synchronized by the Excess Thymidine Block technique and the distribution of G1/S phases was determined by flow cytometry (FACS). The hypoxic-cell model of G1-phase RTEs was established by exposure of the RTEs to sodium cyanide (NaCN), and the preventive (NaCN+Cantharidin) group and un-treated control group were set up at the same time.

[Cantharidin regulates intracellular ATP in G1/S-phase renal tubule epithelial cells].

Objective: To study the mechanism of cantharidin in protecting F-actin microfilaments from disruption by hypoxic damage by observing the effects of cantharidin on intracellular ATP metabolism in G(1)/S-phase renal tubule epithelial cells (RTECs).

Methods: G1-phase RTECs were divided into cantharidin-treated group, exposed to sodium cyanide (CN) and cantharidin, hypoxic-group with CN exposure and non-treated control group. ATP levels were measured in the 3 groups with high-performance liquid chromatography.