[Experimental study of Xuefu Zhuyu Decoction induced participation of endothelial progenitor cells in the angiogenesis of the ischemic region].

Objective: To observe the effects of Xuefu Zhuyu Decoction (XFZYD) on the angiogenesis and injury repair in the ischemic region.

Methods: The ischemic hind limb rat model was established using Bletilla embolization. Endothelial progenitor cells (EPCs) were labeled with DAPI and injected into the model rats from the vena caudalis.

[Effects of Xuefu Zhuyu decoction on EPCs function].

Objective: To observe the effect on EPCs function by Xuefu Zhuyu Decoction.

Methods: After induced by serial concentrations of Xuefu Zhuyu Decoction-contained serum (XZDCS) and blank serum, EPCs proliferation, migration, adhesion and uptake function were detected by MTT, Boyden chamber, adhesion and uptake of ac-LDL respectively.

Results: Compared with the control group, 15% and 10% XZDCS could elevate the cell regeneration for 24 h and 48 h respectively.

The effect of Xuefu Zhuyu Decoction on in vitro endothelial progenitor cell tube formation.

Objective: To observe the effect of Xuefu Zhuyu Decoction ()-containing serum (XFZYD-CS) on endothelial progenitor cell (EPC) tube formation in vitro.

Methods: Mononuclear cells from rat bone marrow were prepared in a Ficoll density gradient centrifuge. EPCs were separated by the differential attachment method, and observed with inverted microscope for the effect of XFZYD-CS on EPC tube formation.

[Preparation of sustained release multivesicular liposome for thymopentin and preliminary study on its pharmacokinetics in rats].

To optimize the formulation and preparation method of multivesicular liposome of thymopentin and to investigate its pharmacokinetics in rats, the multivesicular liposome of thymopentin was prepared by double emulsification method and the formulation was optimized by orthogonal design. The release characteristics of thymopentin from multivesicular liposome in PBS (pH 7.4) and in plasma were investigated.