gene disruption causes severe limb deformities in pigs.

In an attempt to generate g.A746G substitution in the gene, we unexpectedly obtained homozygous knockout piglets ( ) and heterogeneous knockout piglets with one copy of the A746G mutation ( ) via CRISPR/Cas9 editing. Polymerase chain reaction (PCR) and sequencing revealed complex genomic rearrangements in the target region.

[Application of CRISPR/Cas9 mediated genome editing in farm animals].

CRISPR (Clustered regularly interspaced short palindromic repeats)/Cas (CRISPR associated proteins) is an acquired immune system found in bacteria and archaea that fight against invasion of viruses or plasmids. CRISPR/Cas systems are currently classified into three main types: I, II and III, of which type II has relatively simple components. The CRISPR/Cas9 technology modified from type II CRISPR/Cas system has been developed as an efficient genome editing tool.