MiR-124-5p Inhibits the Progression of Gastric Cancer by Targeting MIEN1.

Objective: To observe the effect of miR-124-5p on progression of gastric cancer (GC) and explore the targeting mechanism.

Methods: After collecting the specimens, we used real-time fluorescence quantitative PCR to detect the miR-124-5p level of GC tissue and corresponding adjacent tissue. Then MTT test and scratch wound-healing assay were hired to evaluate the influence of miR-124-5p in GC cell (SGC-803 and SGC7901) migration and proliferation ability.

Mucosal fluid evaporation is not the method of heat dissipation from fourth-degree laryngopharyngeal burns.

This study was designed to explore whether mucosal fluid evaporation represents a method of heat dissipation from thermal air inhalation injury and to assess laryngopharyngeal tissue damage according to heat quantity changes of dry air and vapour. Fifteen adult male beagles were divided into five groups to inhale heated air or vapour for 10 min as follows: control group (ordinary air), group I (91-110 °C heated air), group II (148-175 °C heated air), group III (209-227 °C heated air), and group IV (96 °C saturated vapour). The heat quantity changes of the dry air and vapour were calculated via thermodynamic formulas.

Laryngeal morphologic changes and epidemiology in patients with inhalation injury: a retrospective study.

Background And Objectives: Laryngeal morphologic changes are important in risk assessment of upper airway obstruction (UAO) after inhalation injury. This retrospective study evaluates the clinical application of laryngeal burn classification system.

Materials And Methods: Clinical data from January 1999 to June 2013 were analyzed retrospectively.

The Preparation of Acellular Dermal Matrices by Freeze-Thawing and Ultrasonication Process and the Evaluation of Its Antigenicity.

Antigenicity is the biggest obstacle of xenogeneic acellular dermal matrices (ADM) as dermal scaffold in treatment of large-area skin defect. We prepared ADM by repeated freezing and thawing and ultrasonic process, and then injected the ADM homogenate and degradation product into porcine skin to evaluate the effectiveness of the decellularized method and the antigenicity of porcine ADM. In this work, chinese miniature pigs (n = 10) were sensitized by subcutaneous injection with human ADM degradation products on days 0, 7, and 14.