Identification and characterization of two types of amino acid-regulated acetyltransferases in actinobacteria.

One hundred and fifty GCN5-like acetyltransferases with amino acid-binding (ACT)-GCN5-related -acetyltransferase (GNAT) domain organization have been identified in actinobacteria. The ACT domain is fused to the GNAT domain, conferring amino acid-induced allosteric regulation to these protein acetyltransferases (Pat) (amino acid sensing acetyltransferase, (AAPatA)). Members of the AAPatA family share similar secondary structure and are divided into two groups based on the allosteric ligands of the ACT domain: the asparagine (Asn)-activated PatA and the cysteine (Cys)-activated PatA.

[Efficiency of Aerobic Co-composting of Urban Sludge and Chinese Medicinal Herbal Residues].

With Chinese medicinal herbal residues and municipal sludge as raw materials for co-composting experiment, the effect of the material ratio and addition time of Chinese medicinal herbal residues on the composting efficiency were investigated, including the change of the temperature, organic matter, ammonia nitrogen, and activity of protease. The best composting conditions were determined based on the results. The experimental results showed that the temperature of the pile was raised in the presence of 60 g Chinese medicinal herbal residues as carbon source and 300 g municipal sludge, the ammonia volatilization was reduced and the activity of protease was improved.