Gene Expression of Ethanol and Acetate Metabolic Pathways in the EmaSR Regulon.

Background: Previous studies have confirmed the involvement of EmaSR (ethanol metabolism a sensor/regulator) in the regulation of ATCC 19606 ethanol and acetate metabolism. RNA-seq analysis further revealed that , , , and regulatory gene clusters potentially participate in ethanol and acetate metabolism under the control of EmaSR.

Methods: This study fused the EmaSR regulon promoter segments with reporter genes and used fluorescence expression levels to determine whether EmaSR influences regulon expression in ethanol or acetate salt environments.

Reactivity of human antisera to codon optimized SARS-CoV2 viral proteins expressed in .

Objective: The coronavirus disease 2019 (COVID-19) pandemic caused by the SARS-CoV2 virus continues to pose a serious threat to public health worldwide. The development of rapid diagnostic kits can assist the Tzu Chi Foundation in supporting global volunteers working to provide relief during the current pandemic.

Materials And Methods: In this study, nucleotide sequences derived from publicly available viral genome data for several domains of the SARS-CoV2 spike and nucleocapsid (N) proteins were chemically synthesized, with codon optimization for protein expression.

Transcranial color-coded sonography helps differentiation between idiopathic Parkinson's disease and vascular parkinsonism.

Background: Recently, transcranial color-coded sonography (TCCS) has been found to have a diagnostic value in patients with idiopathic Parkinson's disease (IPD), which displays increased hyperechogenicity at the substantia nigra (SN).

Objective: To use TCCS, to assess the difference in SN hyperechogenicity and intracranial hemodynamics among subjects with IPD, vascular parkinsonism (VP) and controls.

Methods: Eighty IPD and 30 VP patients, and 60 controls were recruited into this study.