Development of the near-infrared fluorescence probe for detection of ONOO and its application in vitro and vivo.

Diabetes has become one of the most common endocrine and metabolic diseases threatening human health, which can induce mitochondrial dysfunction and exacerbate the excessive production of reactive oxygen species (ROS). Among them, ONOO level fluctuation was closely related to diabetes. Hence, it is of great significance to develop a near-infrared fluorescence probe for visualizing ONOO level fluctuations in diabetes.

A fluorescence probe with targeted mitochondria for detecting hydrogen peroxide and in diabetic mice.

We designed and prepared probe W-1 for the detection of HO. W-1 showed excellent selectivity for HO and was accompanied by colorimetric signal changes. The excellent linear relationship between fluorescence intensity and HO concentration (0-100 μM) provided favorable conditions for its quantitative detection.

The highly selective and sensitive fluorescence probe for detection of copper (II) ions and its bioimaging in vitro and vivo.

We designed and developed the probe W-3 for detection of Cu. The results showed probe can selectively detect Cu, accompanied by noticeable color change. The probe can detect the Cu in water samples and drinks based on absorption detection.

Multifunctional fluorescence probe for simultaneous detection of viscosity, polarity, and ONOO and its bioimaging in vitro and vivo.

Intracellular microenvironment (viscosity and polarity) and peroxynitrite ions (ONOO) are involved in maintaining cell morphology, cell function, and signaling so that it is crucial to explore their level changes in vitro and vivo. In this work, we designed and synthesized a mitochondria-targeted fluorescence probe XBL for monitoring the dynamic changes of viscosity, polarity, and ONOO based on TICT and ICT mechanism. The fluorescence spectra showed obvious changes for polarity at 500 nm as well as ONOO and viscosity at 660 nm, respectively.