The complete chloroplast genome sequence of (Primulaceae) and its phylogeny.

W.B Ju, B. Xu & X.

Correlation between DNA methylation and Thymic Stromal Lymphopoietin expression in asthmatic airway epithelial cells.

Background: The overexpression of TSLP and DNA methylation in asthma were both risk factors the relationship was not clear.

Objective: This study aimed to investigate the relationship between methylation status of TSLP promoter and mRNA/protein expression in asthmatic airway epithelial cells.

Methods: Human bronchial epithelial cells were cultured in vitro and divided into: Control group, treated with PBS, model group, sensitized with LPS (10 μg/mL) for 12 h (37 °C, 5% CO).

Simultaneous Determination of 5 Components in the Leaves of by Quantitative Analysis of Multicomponents by Single Marker (QAMS) Based on UPLC and HPLC.

The pharmacodynamic effect of leaves was attributed to various components, especially the flavonoids. In this paper, a new strategy of quantitative analysis of multicomponents by a single marker (QAMS) method was first established to synchronously determine 5 components (ethyl gallate (C), astragalin (C), quercetin (C), luteolin (C), and kaempferol (C)) in by ultra-performance liquid chromatography (UPLC) and high-performance liquid chromatography (HPLC). Quercetin (C) was chosen as the internal reference.

Sphingosine kinase 1/sphingosine 1-phosphate signalling pathway as a potential therapeutic target of pulmonary hypertension.

Pulmonary hypertension is characterized by extensive vascular remodelling, leading to increased pulmonary vascular resistance and eventual death due to right heart failure. The pathogenesis of pulmonary hypertension involves vascular endothelial dysfunction and disordered vascular smooth muscle cell (VSMC) proliferation and migration, but the exact processes remain unknown. Sphingosine 1-phosphate (S1P) is a bioactive lysophospholipid involved in a wide spectrum of biological processes.

Transfer of the IL-37b gene elicits anti-tumor responses in mice bearing 4T1 breast cancer.

Aim: IL-37b has shown anti-cancer activities in addition to its anti-inflammatory properties. In this study, we investigated the effects of IL-37b on breast carcinoma growth in mice and to determine the involvement of T cell activation in the effects.

Methods: IL-37b gene was transferred into mouse breast carcinoma cell line 4T1 (4T1-IL37b cells), the expression of secretory IL-37b by the cells was detected, and the effects of IL-37b expression on the cell proliferation in vitro was evaluated.

RAGE signaling significantly impacts tumorigenesis and hepatic tumor growth in murine models of colorectal carcinoma.

Background: The receptor for advanced glycation end-products (RAGE) is a cell surface receptor implicated in tumor cell proliferation and migration. We hypothesized that RAGE signaling impacts tumorigenesis and metastatic tumor growth in murine models of colorectal carcinoma.

Materials And Methods: Tumorigenesis: Apc (1638N/+) mice were crossed with Rage (-/-) mice in the C57BL/6 background to generate Apc (1638N/+)/Rage (-/-) mice.

Simultaneous removal of coexistent heavy metals from simulated urban stormwater using four sorbents: a porous iron sorbent and its mixtures with zeolite and crystal gravel.

The selectivity sequence and removal of coexistent heavy metals (namely As, Cd, Cr, Cu, Ni and Zn) in synthetic urban stormwater runoff were investigated by adsorption onto a porous iron sorbent (namely P1) and its mixtures with zeolite and crystal gravel, respectively (namely P2, P3, and P4). A batch method was employed to simulate the sorption processes. The geochemical model PHREEQC was used to calculate the metals' species and saturation data for elucidating the sorption data.

[Determination of trace elements of Gentiana macrophlla and Gentiana straminea by microwave digestion-FAAS].

An acid-assisted microwave digestion procedure is optimized for the determination of trace elements in traditional Chinese medicine by the use of flame atomic absorption spectrometric (FAAS) techniques. Microwave-assisted digestion has the advantages of reduced time for sample dissolution, fewer possibilities for technical errors caused by spilling of hot digestion solutions, use of less chemicals, and lower losses of volatile metals. In addition, modern microwave ovens are safer and simpler and provide more controlled and reproducible conditions than hot plate or block digesters.