[Estrogens determination of livestock dung based on UE-SPE-HPLC/FLD].

A method for detecting the estrogens estriol, 17beta-estradiol, ethinyl estradiol, and bisphenol A in livestock dung was established by the combination of ultrasonic extraction (UE), solid phase extraction (SPE) purification, and high performance liquid chromatography (HPLC) with fluorescence detector (FLD). The dung samples were extracted with ethyl acetate ultrasonication for 30 min, and purified with C18 solid phase extraction column and related solvents. The test four estrogens in the dung samples were isolated with Inertsil ODS-SP-C18 reversed-phase columns (150 mm x 4.

[Analysis on 2175 admitted cases of clonorchiasis sinensis in Guigang City].

Objective: To understand the epidemiological features of the hospitalized cases with Clonorchis sinensis infection in Guigang City of Guangxi Region.

Methods: Fecal samples were collected from patients each with 30-50 g and examined by sedimentation technique. Number of eggs was counted in randomly selected cases by Stoll's method.