Publications by authors named "Yu-Kun Jie"
Hypoxia-inducible factors -1 (HIF-1) is a crucial transcription factor that regulates the expression of glycolytic genes. Our previous study proved that the Mud crab dicistrovirus-1 (MCDV-1) can induce aerobic glycolysis that favors viral replication in mud crab Scylla paramamosain. However, the role of HIF-1 on key glycolytic genes during the MCDV-1 infection has not been examined.
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- Prolyl hydroxylase 2 (PHD2) serves as the main oxygen sensor regulating HIF-1α stability, and researchers identified a novel PHD2 gene called SpPHD2 from the mud crab Scylla paramamosain, revealing its full transcript length of 1926 bp.
- The SpPHD2 protein features key structural domains and is broadly expressed in various tissues, with its localization primarily in the cytoplasm.
- Silencing SpPHD2 increased levels of SpHIF-1α and associated genes, while overexpression decreased SpHIF-1α activity; additionally, SpPHD2 expression rises under low oxygen conditions, suggesting a feedback loop in regulating hypoxia responses.
Glutaredoxin (Grx) is a glutathione-dependent oxidoreductase that plays a key role in antioxidant defense. In this study, a novel Grx2 gene (SpGrx2) was identified from the mud crab Scylla paramamosain, which consists of a 196 bp 5' untranslated region, a 357 bp open reading frame, and a 964 bp 3' untranslated region. The putative SpGrx2 protein has a typical single Grx domain with the active center sequence C-P-Y-C.
View Article and Find Full Text PDFHypoxia is a major environmental stressor that can damage the oxidation metabolism of crustaceans. Glutaredoxin (Grx) is a key member of the thioredoxin superfamily and plays an important role in the host's defense against oxidative stress. At present, the role of Grx in response to hypoxia in crustaceans remains unclear.
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- Phosphofructokinase (PFK) is a crucial enzyme in glycolysis, and researchers have cloned and studied a specific PFK gene (SpPFK) from the mud crab Scylla paramamosain.
- The SpPFK gene consists of 249 bp of 5' UTR, a 2,859 bp open reading frame, and 1,248 bp of 3' UTR, with high expression levels in the gill, hemocytes, and muscle, especially after infection with mud crab dicistrovirus-1 (MCDV-1).
- Knocking down SpPFK through RNA interference was shown to lower lactate dehydrogenase levels, enhance survival rates in
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- Apoptosis is crucial for the immune defense against infections, and caspase 3, a family of proteins, plays a key role in this process.
- The full-length caspase 3 from mud crab (Sp-caspase 3) was cloned, characterized, and found to be expressed widely, especially in hemocytes, with its activity located in both the cytoplasm and nucleus.
- Over-expressing Sp-caspase 3 can induce cell death and its expression increases during infection with V. Parahaemolyticus, while knocking it down reduces apoptosis and increases crab mortality.
Oxidative stress is considered as the toxicity mechanism of environmental stressors on aquatic organisms. This study aims to explore the effects of oxidative stress on physiological responses, DNA damage and transcriptional profiles of the mud crabs Scylla paramamosain. In the present study, mud crabs were injected with 0.
View Article and Find Full Text PDFHypoxia-induced oxidative stress and transcriptome changes in the mud crab (Scylla paramamosain).
Comp Biochem Physiol C Toxicol Pharmacol
July 2021
Mud crab (Scylla paramamosain) is an economically important cultured species in China. Hypoxia is a major environmental stressor during mud crab culture. In the present study, we investigated the oxidative stress and transcriptome changes in the gills of mud crab after intermediate hypoxia stress with dissolved oxygen (DO) 3.
View Article and Find Full Text PDFCadmium is one of the most common heavy metal pollutants in the aquatic environment. Mud crab (Scylla paramamosain) is considered a model organism to monitor the impact of heavy metals. However, knowledge about toxicological mechanism of cadmium in crustaceans still remains limited.
View Article and Find Full Text PDFImmune and physiological responses of mud crab (Scylla paramamosain) under air exposure.
Comp Biochem Physiol C Toxicol Pharmacol
July 2020
Article Synopsis
- Researchers examined how mud crabs (Scylla paramamosain) respond physiologically and immunologically to being exposed to air.
- Air exposure led to increased levels of certain plasma enzymes (AST, ALT, ALP) and elevated glucose and malondialdehyde levels, indicating stress.
- Key proteins and signals, including SOD, CAT, HSP90, HSP70, and HIF-1, were activated, showing oxidative stress was a factor, especially after prolonged air exposure, with notable fluctuations in caspase-3 levels over time.
Nitrite is one of major environmental pollutants that can impact immunological parameters in aquatic organisms. In the present study, we investigated the effects of nitrite exposure on oxidative stress, DNA damage and apoptosis in mud crab (Scylla paramamosain). Mud crab were exposed to 0, 5, 10 and 15 mg L nitrite for 72 h.
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