A Temperature-Sensitive Luminescent Ag Nanocluster Supported by TertButyl Thiol Ligands.

Compound [Ag S (StBu) (CF COO) (CO )](CO ) ⋅CH Cl ⋅4CH OH⋅9DMF (1) has been obtained and well defined. It consists of a multi-shell structure involving two Ag centres, one Ag S pentagram, two Ag S pentagons and one Ag S shell. Compound 1 has been characterized by XPS, FT-IR, PXRD, TGA, NMR, MS, UV/Vis spectrum, TEM and cyclic voltammetry.

Activation of chemokine receptor CXCR4 in malignant glioma cells promotes the production of vascular endothelial growth factor.

Numerous studies have showed that chemokine receptors, such as CXCR4, contribute to the growth and metastasis of a variety of malignant tumors. In this study, we investigated the role of CXCR4 in the production of angiogenic factor, vascular endothelial growth factor (VEGF), in various human glioma cells from astrocytic origin. The expression of CXCR4 mRNA and protein in three glioma cell lines, U87-MG, SHG-44, and CHG-5, was determined by RT-PCR and immunocytochemistry, respectively.

[Expression of augmenter of liver regeneration in hepatic tumor cells and its clinical significance].

Objective: To investigate the effects of augmenter of liver regeneration (ALR) on the proliferation of hepatocytes and hepatic tumor cells and the expression of ALR in herpatocellular carcinoma (HCC).

Methods: Primary rat hepatocytes, QGY and HepG2 cells were cultured separately with ALR from different species. Cell proliferation was detected by their 3H-TdR uptake.

Expression of human augmenter of liver regeneration in pichia pastoris yeast and its bioactivity in vitro.

Aim: To construct a yeast expression system of human augmenter of liver regeneration (hALR) and to examine its bioactivity in vitro.

Methods: With PCR and gene recombination techniques, cDNA of open reading frame of hALR was obtained from recombinant plasmid pcDNA3.1-hALR and inserted into plasmid pPIC9.

[Expression of rat augmenter of liver regeneration in pichia pastoris and evaluation of its bioactivity in vitro].

Objectives: To construct yeast expression plasmid of rat augmenter of liver regeneration (rALR), express it in GS115, and examine its bioactivity in vitro.

Methods: With PCR and genetic recombination techniques, the gene fragment of rALR was amplified from recombinant plasmid pcDNA3.1-rALR, and the recombinant plasmid pPIC9K-rALR was constructed.