Publications by authors named "Yu-Hai Gao"

Objective: To explore effects of isopsoralen (ISO) with different doses on fracture and vascular healing in mice.

Methods: Sixty 2-month-old male C57BL/6 mices with body mass of (20±2) g were selected and divided into 4 groups by random number table method:model group (model), low dose group (isopsoralen-low dose, ISO-L), medium dose group (isopsoralen-medium dose, ISO-M) and high dose group (isopsoralen-high dose, ISO-H), with 15 animals in each group. The right tibial fracture model was established.

View Article and Find Full Text PDF
Article Synopsis
  • Oxidative stress is linked to bone loss during spaceflight, with microgravity causing shortening of primary cilia in osteoblasts, leading to increased oxidative stress and decreased bone formation.
  • The study found that this oxidative stress is associated with mitochondrial dysfunction and intracellular calcium overload, primarily driven by the TRPV4 calcium channel.
  • Protecting primary cilia through specific interventions, such as miR-129-3p overexpression or flavonoid supplementation, can prevent the negative impacts of microgravity on oxidative stress and bone health.
View Article and Find Full Text PDF

The molecular mechanism for the microgravity-induced decrease in bone formation remains unclear and there is a lack of effective specific preventative therapies. We recently reported that primary cilia of osteoblasts became shorter and even disappeared when the cells were exposed to random positioning machine (RPM)-simulated microgravity and that the microgravity-induced loss of osteogenic potential of osteoblasts could be attenuated when the resorption of primary cilia was prevented by treatment with 0.1 μM cytochalasin D.

View Article and Find Full Text PDF
Article Synopsis
  • PEMFs are safe and effective for treating bone issues like nonunion and osteoporosis, but their exact working mechanisms are not fully understood.
  • The study found that PEMFs promote bone cell differentiation via a specific signaling pathway that requires primary cilia and nitric oxide (NO).
  • Blocking the NO signaling pathway prevents PEMFs from elongating primary cilia or stimulating bone cell differentiation, highlighting the interdependent relationship between NO and primary cilia in bone formation.
View Article and Find Full Text PDF

Electromagnetic fields (EMFs) have emerged as a versatile means for osteoporosis treatment and prevention. However, its optimal application parameters are still elusive. Here, we optimized the frequency parameter first by cell culture screening and then by animal experiment validation.

View Article and Find Full Text PDF

Objective: To compare effects of low frequency pulsed electromagnetic fields on bone quality in growing rats between 1 h and 1.5 h.

Methods: Thirty male SPF SD rats aged 4 weeks selected, which with the average body weight (115.

View Article and Find Full Text PDF

Objective: Subclavian steal syndrome results from hemodynamic impairment due to stenosis or occlusion of subclavian artery. Therefore, it is important for subclavian steal syndrome patients to assess hemodynamic status during endovascular therapy.

Methods: Eleven subclavian steal syndrome patients undergoing endovascular therapy attended this study.

View Article and Find Full Text PDF

To investigate the preventive effect and possible mechanism of puerarin(Pur) in rat model of disuse osteoporosis(DOP),thirty healthy Wistar female rats of 2 months old were randomly divided into control group(Control), hindlimb suspension group(HLS), and puerarin group(HLS+Pur) in hindlimb suspension, with 10 rats in each group. A disuse osteoporosis model was established by tail suspension method, and 15.4 mg·kg~(-1) puerarin suspension was administered to HLS+Pur group every day, and the same volume of distilled water was administered to Control group and HLS group respectively.

View Article and Find Full Text PDF

Objective To compare the effects of 50-Hz 0.6-mT low-frequency pulsed electromagnetic fields(PEMFs) and 50-Hz 1.8-mT sinusoidal alternating electromagnetic fields(SEMFs) in preventing bone loss in tail-suspended rats,with an attempt to improve the prevention and treatment of bone loss caused by weightlessness.

View Article and Find Full Text PDF

Extremely low-frequency electromagnetic fields have been considered a potential candidate for the prevention and treatment of osteoporosis; however, their action mechanism and optimal magnetic flux density (intensity) parameter are still elusive. The present study found that 50-Hz sinusoidal electromagnetic fields (SEMFs) at 1.8 mT increased the peak bone mass of young rats by increasing bone formation.

View Article and Find Full Text PDF

Microgravity is one of the main threats to the health of astronauts. Pulsed electromagnetic fields (PEMFs) have been considered as one of the potential countermeasures for bone loss induced by space flight. However, the optimal therapeutic parameters of PEMFs have not been obtained and the action mechanism is still largely unknown.

View Article and Find Full Text PDF
Article Synopsis
  • The study aimed to investigate how a combination of tanshinone 2A and resveratrol affects bone mass in growing rats, focusing on potential anti-osteoporosis mechanisms from traditional Chinese medicine.
  • 40 one-month-old female Wistar rats were divided into four groups receiving different treatments, with body weight and bone mineral density measured regularly over two months.
  • Results showed significant improvements in bone density and quality for the compound group compared to the control, indicated by increased levels of osteocalcin and tartrate-resistant acid phosphatase 5b.
View Article and Find Full Text PDF
Article Synopsis
  • The study aimed to compare the effects of resveratrol, puerarin, and a combination of both on peak bone mass in rats.
  • After dividing rats into four groups (control, puerarin, resveratrol, and a compound group), the researchers monitored body weight and measured bone density over three months, concluding that both resveratrol and puerarin significantly enhanced bone mineral density compared to the control group.
  • The combined treatment of resveratrol and puerarin showed the most considerable improvements in bone structure and strength, highlighting the potential of these compounds for enhancing bone health.
View Article and Find Full Text PDF

The application of pulsed electromagnetic fields (PEMFs) in the prevention and treatment of osteoporosis has long been an area of interest. However, the clinical application of PEMFs remains limited because of the poor understanding of the PEMF action mechanism. Here, we report that PEMFs promote bone formation by activating soluble adenylyl cyclase (sAC), cyclic adenosine monophosphate (cAMP), protein kinase A (PKA), and cAMP response element-binding protein (CREB) signaling pathways.

View Article and Find Full Text PDF
Article Synopsis
  • Epimedium sagittatum brevicornum is a traditional Chinese herb known for its role in healing fractures and treating osteoporosis.
  • This study aimed to explore whether the total flavonoid extract (TFE) from Epimedium could increase peak bone mass in young rats, which is crucial for preventing osteoporosis later in life.
  • Results showed that TFE at middle and high doses significantly enhanced bone mineral density and improved bone properties, promoting bone formation through a specific biochemical pathway without affecting bone resorption.
View Article and Find Full Text PDF

Objective: To study effects of 50 Hz 1.8 mT sinusoidal electromagnetic fields (SEMFs) on bone mineral density (BMD) in SD rats.

Methods: Thirty SD rats weighted(110±10) and aged 1 month were randomly divided into control group and electromagnetic field group, 15 in each group.

View Article and Find Full Text PDF

Pulsed electromagnetic fields (PEMFs) have been considered as a potential candidate for the prevention and treatment of osteoporosis, however, the mechanism of its action is still elusive. We have previously reported that 50Hz 0.6mT PEMFs stimulate osteoblastic differentiation and mineralization in a primary cilium- dependent manner, but did not know the reason.

View Article and Find Full Text PDF

Objective: To establish a collagen hydrogel three-dimensional culture model with rat calvarial osteoblasts (ROBs).

Methods: ROBs were obtained through enzyme digestion of segregated neonatal SD rat skull. The collagen hydrogel three-dimensional culture model was established by mixing ROBs with different concentrations of type I rat tail collagen (collagen concentration of 1, 2, 3 mg/mL), DMEM medium and NaOH under adjusted PH and a temperature of 37 degrees C.

View Article and Find Full Text PDF

Objective: To investigate osthole effect on femoral tissue resorption activity of rat in vitro.

Methods: Six SD rats weighted (80 ± 5) g were used to isolate and culture femoral tissue (diaphyses and metaphysis) in vitro. The cultured tissue were devided into control group, estradiol group and osthole group.

View Article and Find Full Text PDF

Objective: To investigate the effects of icariin on the differentiation and maturation of rat calvarial osteoblasts(ROB) in collagen hydrogel three-dimensional culture.

Methods: ROB were obtained by enzyme digestion from the segregated neonatal SD rats skull and were embedded in 2 mg/mL rat tail collagen for three-dimensional culture. The growth state of ROB was observed by FDA/PI staining, HE staining and scanning electron microscopy.

View Article and Find Full Text PDF

Although pulsed electromagnetic fields (PEMFs) have been approved as a therapy for osteoporosis, action mechanisms and optimal parameters are elusive. To determine the optimal intensity, exposure effects of 50 Hz PEMFs of 0.6-3.

View Article and Find Full Text PDF

Objective: To investigate the effects of different-intensity sinusoidal electromagnetic fields (SEMFs) on bone mineral density (BMD) and histomorphometry in SD rats.

Methods: Thirty female SD rats were randomly divided into three groups: group A (a control group), group B (0.1 mT group) and group C (0.

View Article and Find Full Text PDF

Objective: To investigate the effect of 50 Hz 0.1 mT sinusoidal electromagnetic field at different time points on bone mineral density(BMD)and histomorphometry in rats.

Methods: Totally 50 6-week-old female SD rats were equally randomized into 5 groups: control group,45-minute group,90-minute group,180-minute group,and 270-minute group.

View Article and Find Full Text PDF

Objective: Through researching the relationship among osteoporosis and inflammatory reaction besides angiogenesis, to compare pharmacological differences between icariin and genistein to inhibit bone loss.

Methods: 6 months old female SD rats were randomly divided into SHAM group, model group, ICA group, GEN group and E group. The bone mineral density of total, femur and lumbar, serum OC, TRACP 5b, IL-6 and VEGF, biomechanics of femur and tibia microarchitecture were analyzed.

View Article and Find Full Text PDF

Effects of sinusoidal electromagnetic fields (SEMFs) on bone metabolism have not yet been well defined. The present study investigated SEMF effects on bone formation and resorption in rat femur bone tissues in vitro. Cultured femur diaphyseal (cortical bone) and metaphyseal (trabecular bone) tissues were treated with 50 Hz 1.

View Article and Find Full Text PDF

A PHP Error was encountered

Severity: Warning

Message: fopen(/var/lib/php/sessions/ci_session5jhprqab9ugehlu63rjhl9n86uaj40ri): Failed to open stream: No space left on device

Filename: drivers/Session_files_driver.php

Line Number: 177

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once

A PHP Error was encountered

Severity: Warning

Message: session_start(): Failed to read session data: user (path: /var/lib/php/sessions)

Filename: Session/Session.php

Line Number: 137

Backtrace:

File: /var/www/html/index.php
Line: 316
Function: require_once