Simultaneous Determination of Methotrexate Concentrations in Human Plasma and Cerebrospinal Fluid Using Two-Dimensional Liquid Chromatography: Applications in Primary Central Nervous System Lymphoma.

Background: In this study, we aimed to develop a method for the simultaneous quantification of methotrexate (MTX) samples extracted from human plasma and cerebrospinal fluid (CSF), using two-dimensional liquid chromatography (2D-LC). Furthermore, we intended to verify whether intravenous mannitol could increase MTX concentration in the CSF of patients.

Methods: The mobile phase of PUMP1 consisted of 10.

The Accelerated Progression of Atherosclerosis Correlates with Decreased miR-33a and miR-21 and Increased miR-122 and miR-3064-5p in Circulation and the Liver of ApoE-/- Mice with Streptozocin (STZ)-Induced Type 2 Diabetes.

Atherosclerosis is a major risk factor for type 2 diabetes (T2D) mortality. We aim to investigate the changes in miR-21, miR-122, miR-33a and miR-3064-5p in circulation and the liver of ApoE-/- mice with streptozocin (STZ)-induced T2D. Twenty 5-week-old male ApoE-/- mice were randomly assigned to the control (n = 10) and T2D group (n = 10) and intraperitoneally injected with a citrate buffer and streptozotocin (STZ) (40 mg/kg BW) once a day for three consecutive days.

Two new thymol derivatives from .

Two new thymol derivatives () were isolated from the aerial parts of . Their structures were elucidated on the basis of comprehensive spectroscopic data analysis as 8,9-dehydrothymol-3---glucoside (), and 9-(acetyloxy)thymol-3-yl (3-methylbut-2-enoate) (). All isolates were evaluated for cytotoxic activities with IC50 values greater than 50 μM against MCF-7, HeLa, A549, and Hep G-2 cancer cells.

Real world effectiveness of PCSK-9 inhibitors combined with statins versus statins-based therapy among patients with very high risk of atherosclerotic cardiovascular disease in China (RWE-PCSK study).

Background: The efficacy and safety of proprotein convertase subtilisin/kexin type 9 (PCSK-9) inhibitors were confirmed by several clinical trials, but its effectiveness in routine clinical practice in China has not been evaluated. This study aims to describe the real world effectiveness of PCSK-9 inhibitors combined with statins compared with statins-based therapy among patients with very high risk of atherosclerotic cardiovascular disease (ASCVD).

Methods: This is a multi-center observational study, enrolled patients from 32 hospitals who underwent percutaneous coronary intervention (PCI) from January to June in 2019.

Quantum phase transition in a non-Hermitianspin chain with global complex transverse field.

In this work, we investigate the quantum phase transition in a non-Hermitianspin chain. The phase diagram shows that the critical points of Ising phase transition expand into a critical transition zone after introducing a non-Hermitian effect. By analyzing the non-Hermitian gap and long-range correlation function, one can distinguish different phases by means of different gap features and decay properties of correlation function, a tricky problem in traditionalmodel.

Arsenic transfer along the soil-sclerotium-stroma chain in Chinese cordyceps and the related health risk assessment.

Background: Chinese cordyceps (Lepidoptera: )is a larval-fungus complex. The concentration and distribution of arsenic (As) may vary during the stroma (ST) germination process and between the sclerotium (SC) and the ST. The soil-to-Chinese cordyceps system is an environmental arsenic exposure pathway for humans.

Efficient microwave-to-optical single-photon conversion with a single flying circular Rydberg atom.

We propose a scheme for converting a microwave (mw) single photon in a mw cavity to a flying optical photon. The conversion is realized by using a flying circular Rydberg atom, which plays a role of the "data bus" as an excellent memory to connect the mw and optical cavities. To link the energy levels of atom in optical domain and mw domain, we use fast decircularization method and three-photon Raman transition method.

[Characteristics and Removal Mechanism of an Electro-Hybrid Ozonation-Coagulation System in the Treatment of Organic Matters].

To improve the removal efficiency of dissolved organic matter in wastewater treatment plant (WWTP) effluent, electro-hybrid ozonation-coagulation (E-HOC) is proposed and the treatment characteristics and removal mechanism for WWTP effluent and ibuprofen (IBP) are investigated. The E-HOC process has a better removal effect on dissolved organic matter in WWTP effluent, achieving 46.4%, 20.

Quantifying Light Response of Leaf-Scale Water-Use Efficiency and Its Interrelationships With Photosynthesis and Stomatal Conductance in C and C Species.

Light intensity () is the most dynamic and significant environmental variable affecting photosynthesis ( ), stomatal conductance ( ), transpiration ( ), and water-use efficiency (WUE). Currently, studies characterizing leaf-scale WUE- responses are rare and key questions have not been answered. In particular, (1) What shape does the response function take? (2) Are there maximum intrinsic (WUE; WUE) and instantaneous WUE (WUE; WUE) at the corresponding saturation irradiances ( and )? This study developed WUE- and WUE- models sharing the same non-asymptotic function with previously published - and - models.

Comparing two measures of leaf photorespiration rate across a wide range of light intensities.

Suppression of photorespiration by low O concentrations (Method 1) and simultaneous measurements of gas exchange and chlorophyll fluorescence (Method 2) are often used to estimate leaf photorespiration rate (R) of C plants. However, it is largely unknown whether Method 1 and Method 2 can be used equivalently in estimating R. Using a field experiment on two wheat cultivars (T.

Quantification of the Plasma Concentration of Apatinib by 2-Dimensional Liquid Chromatography.

Background: Apatinib is a new oral micromolecular tyrosine kinase inhibitor, which is mainly used as a third-line treatment for chemotherapy-refractory advanced metastatic gastric cancer patients. However, apatinib has shown dose titration and severe adverse reactions in clinical practice. Quantification of plasma concentrations of apatinib may be an effective method to balance the clinical efficacy and adverse reactions.

Recent advances in fluorescent probes for monitoring of hydrogen sulfide.

Hydrogen sulfide (H₂S), known for its unpleasant rotten egg smell and its high toxicity, has recently emerged as an important mediator of human physiological and pathological processes, such as the regulation of cell growth, cardiovascular protection, the stimulation of angiogenesis, gastric mucosal injury and Alzheimer's disease. Due to its significant actions in the physiology, H₂S has attracted the abundant concern of numerous researchers in the cutting edge of chemistry, biology and medicine. Recently, several fluorescent probes have been developed for detecting and elucidating the role played by H₂S in biological systems.

Influence of drought intensity on the response of six woody karst species subjected to successive cycles of drought and rewatering.

Tolerance to the effects of drought and subsequent recovery after a rainfall appear to be critical for plants in the karst regions of southwestern China, which are characterized by frequent but temporary drought events. This study investigated the effects of drought intensity and repetition on photosynthesis and photoprotection mechanisms of karst plants during successive cycles of drought and subsequent recovery. Leaf water potential, gas exchange, chlorophyll fluorescence and several associated metabolic processes were studied in six plant species, including Pyracantha fortuneana (PF), Rosa cymosa (RC), Broussonetia papyrifera (BP), Cinnamomum bodinieri (CB), Platycarya longipes (PL) and Pteroceltis tatarinowii (PT) during three cycles of drought treatments at four different intensities.