Characterization of a heat-tolerant sp. GD mutant with enhanced photosynthetic CO fixation efficiency and its implication as lactic acid fermentation feedstock.

Background: Fermentative production of lactic acid from algae-based carbohydrates devoid of lignin has attracted great attention for its potential as a suitable alternative substrate compared to lignocellulosic biomass.

Results: A sp. GD mutant with enhanced thermo-tolerance was obtained by mutagenesis using -methyl-'-nitro--nitrosoguanidine to overcome outdoor high-temperature inhibition and it was used as a feedstock for fermentative lactic acid production.

Enhanced Ascorbate Regeneration Via Dehydroascorbate Reductase Confers Tolerance to Photo-Oxidative Stress in Chlamydomonas reinhardtii.

The role of ascorbate (AsA) recycling via dehydroascorbate reductase (DHAR) in the tolerance of Chlamydomonas reinhardtii to photo-oxidative stress was examined. The activity of DHAR and the abundance of the CrDHAR1 (Cre10.g456750) transcript increased after moderate light (ML; 750 µmol m s) or high light (HL; 1,800 µmol m s) illumination, accompanied by dehydroascorbate (DHA) accumulation, decreased AsA redox state, photo-inhibition, lipid peroxidation, HO overaccumulation, growth inhibition and cell death.