Publications by authors named "Yu-Di Luo"
Background: Folic acid and zinc supplements have been used to treat male infertility, but their efficacy is still debated.
Objective: To systematically evaluate the effects of folic acid and folic acid plus zinc supplements on sperm characteristics and pregnancy outcomes of infertile men.
Methods: An online systematic search was performed using PubMed, Cochrane Library, and EMBASE databases from inception to August 1, 2022.
Article Synopsis
- Kluyveromyces marxianus, a yeast strain, shows promise for producing yeast extracts due to its effective fermentation and nutritional features.
- The study investigates the process of yeast autolysis, which is critical for extract production, and reveals that vacuum negative pressure and higher temperatures trigger this breakdown, but the molecular mechanisms are not yet fully understood.
- RNA-seq analysis identified numerous differentially expressed genes related to RNA synthesis and protein translation during autolysis, indicating that stress leads to cell damage through activated pathways like ERAD and autophagy, ultimately contributing to yeast cell autolysis.
High cell density culture of baker's yeast FX-2 based on pH-stat coupling with respiratory quotient.
Biotechnol Appl Biochem
May 2019
The high cell density culture of baker's yeast FX-2 was investigated in a 50 L(A) automatic bioreactor. Herein, it was found firstly that the Crabtree effect clearly existed in batch fermentation with higher glucose content, then the critical initial glucose content range (≤2.00 g L ) was reasonably ascertained to effectively avoid Crabtree effect.
View Article and Find Full Text PDFThe computational fluid dynamics (CFD) software package Fluent was utilized to simulate the flow field of Escherichia coli (E. coli) BL21 fermentation in a 50 L automatic bioreactor for producing α-cyclodextrin glycosyltransferase (α-CGTase) in this study. 4-down-pumping propeller (4DPP), 6-curved-blade disc turbine (6CBDT), and Rushton turbine (RT) were assembled to form eight impeller combinations (C1-C8).
View Article and Find Full Text PDFUsing 5 Zn supplementation strategies in a 50 L batch bioreactor named FUS-50L(A), possible correlations among Zn content and addition timing, physiologic activity (PA), halohydrin dehalogenase (HheC) accumulation of Escherichia coli P84A/MC1061 were systematically investigated. First, Zn was confirmed as the significant factor, and its optimal concentration for HheC expression was 3.87 mg/L through fermentation experiments in shaking flasks.
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