Publications by authors named "Yu-Chung Kuo"
We present the results of laboratory-based dengue surveillance in Taiwan for 2005. A phylogenetic study showed that multiple dengue epidemics were caused by three different imported dengue virus (DENV) strains. A strain of DENV-3 (genotype I) imported from the Philippines first appeared in the southern part of Kaohsiung City and later spread to Kaohsiung County from August to December, which resulted in 77 cases of dengue.
View Article and Find Full Text PDFArticle Synopsis
- Airport fever screening in Taiwan from July 2003 to June 2004 detected 40 confirmed cases of dengue.
- Advanced testing methods, including IgM and IgG enzyme-linked immunoassays, real-time polymerase chain reaction, and virus isolation, were used to confirm the cases.
- Of the confirmed patients, 33 (82.5%) were viremic, demonstrating that airport screening can effectively identify imported dengue cases.
A quantitative one-step SYBR Green I-based reverse transcription (RT)-PCR system was developed for the detection and differentiation of four different dengue virus serotypes in acute-phase serum samples. A set of group- and serotype-specific primer pairs was designed against conserved sequences in the core region and evaluated for clinical diagnosis. A linear relationship was obtained between the amount of input RNA and cycle threshold (Ct) value over a range of 10 to 10(7) PFU per ml of cell culture-derived dengue viruses.
View Article and Find Full Text PDF