Development and validation of a green and sustainable procedure for the preparation of Perilla frutescens extracts.

A procedure combining supercritical CO and ultrasound-assisted (USC-CO) extraction was developed to obtain rosmarinic acid (RA)-rich extracts from Perilla frutescens. Based on extraction yields and efficiencies, USC-CO was considered the best extraction method among the methods studied for obtaining RA from P. frutescens.

A green approach for the extraction and characterization of oridonin and ursolic and oleanolic acids from Rabdosia rubescens and its kinetic behavior.

An ultrasound-assisted supercritical carbon dioxide (USC-CO) procedure was developed for the extraction of ursolic acid, oleanolic acid and oridonin from Rabdosia rubescens, with yields that were 9.84-10.46 and 15.

Development and characterization of a green procedure for apigenin extraction from Scutellaria barbata D. Don.

This study compared the use of ultrasound-assisted supercritical CO (USC-CO) extraction to obtain apigenin-rich extracts from Scutellaria barbata D. Don with that of conventional supercritical CO (SC-CO) extraction and heat-reflux extraction (HRE), conducted in parallel. This green procedure yielded 20.

Regulatory Efficacy of Brown Seaweed Lessonia nigrescens Extract on the Gene Expression Profile and Intestinal Microflora in Type 2 Diabetic Mice.

Scope: In this study, the antidiabetic activity of Lessonia nigrescens ethanolic extract (LNE) is investigated in streptozotocin (SZT)-induced type 2 diabetic mice fed with a high-sucrose/high-fat diet.

Methods And Results: Ultra high performance liquid chromatography coupled with photo-DAD and electospray ionization-mass spectrometry (ESI-MS) is employed to analyze the major compounds in LNE. The components of the intestinal microflora in type 2 diabetic mice are analyzed by high-throughput next-generation 16S rRNA gene sequencing.

Hepatoprotective effects of raspberry (Rubus coreanus Miq.) seed oil and its major constituents.

Raspberry seed is a massive byproduct of raspberry juice and wine but usually discarded. The present study employed a microwave-assisted method for extraction of raspberry seed oil (RSO). The results revealed that omega-6 fatty acids (linoleic acid and γ-linolenic acid) were the major constituents in RSO.

Evaluation of occupation hot exposure in industrial workplaces in a subtropical country.

Objectives: The objective of this study has been to evaluate the occupational heat exposure of 12 workers at 5 plants in a subtropical country.

Material And Methods: The heat stresses and strain on workers in 5 plants were assessed by the International Organization for Standardization (ISO) 7243 index (wet bulb globe temperature - WBGT) and the ISO 7933 index (maximum allowable exposure time - D).

Results: Results indicated that 42% of the subjects (5 workers) surpassed the WBGT limits.

Production of oridonin-rich extracts from Rabdosia rubescens using hyphenated ultrasound-assisted supercritical carbon dioxide extraction.

Background: Among active components in Rabdosia rubescens, oridonin has been considered a key component and the most valuable compound because it has a wide range of activities beneficial to human health. To produce a high-quality oridonin extract, an alternative hyphenated procedure involving an ultrasound-assisted and supercritical carbon dioxide (HSC-CO ) extraction method to extract oridonin from R. rubescens was developed in this study.

Extraction of α-humulene-enriched oil from clove using ultrasound-assisted supercritical carbon dioxide extraction and studies of its fictitious solubility.

Clove buds are used as a spice and food flavoring. In this study, clove oil and α-humulene was extracted from cloves using supercritical carbon dioxide extraction with and without ultrasound assistance (USC-CO2 and SC-CO2, respectively) at different temperatures (32-50°C) and pressures (9.0-25.

5-Fluorouracil-Induced Apoptosis Changes in Cultured Corneal Epithelial Cells.

Purpose: Repeated subconjunctival injections with 5-fluorouracil (5-FU) after trabeculectomy are used in glaucoma patients for the inhibition of overproliferation in wound site. Thus, a certain amount of the drug may penetrate into epithelial layer, where it causes toxicity to corneal epithelial cells. The aim of this study was to evaluate the toxic effects of 5-FU and mechanisms of drug-induced apoptosis in cultured corneal epithelial cells.

Determination of Oleanolic and Ursolic Acids in Hedyotis diffusa Using Hyphenated Ultrasound-Assisted Supercritical Carbon Dioxide Extraction and Chromatography.

Oleanolic acid (OA) and ursolic acid (UA) were extracted from Hedyotis diffusa using a hyphenated procedure of ultrasound-assisted and supercritical carbon dioxide (HSC-CO2) extraction at different temperatures, pressures, cosolvent percentages, and SC-CO2 flow rates. The results indicated that these parameters significantly affected the extraction yield. The maximal yields of OA (0.

Toona sinensis leaf extract has antinociceptive effect comparable with non-steroidal anti-inflammatory agents in mouse writhing test.

Background: The antinociceptive effect of an aqueous extract from the leaves of Toona sinensis (TS, [A. Juss., M.

Development and validation of a modified ultrasound-assisted extraction method and a HPLC method for the quantitative determination of two triterpenic acids in Hedyotis diffusa.

In the present study, the oleanolic acid (OA) and ursolic acid (UA) contents ofHedyotis diffusa and H. corymbosa were determined by a rapid, selective and accurate method combining modified ultrasound-assisted extraction (MUAE) and HPLC. Compared with traditional extraction methods, MUAE reduced the extraction time, the extraction temperature and the solvent consumption and maximized the extraction yields of OA and UA.

Ultrasound-assisted extraction and quantitation of oils from Syzygium aromaticum flower bud (clove) with supercritical carbon dioxide.

This study evaluated ultrasound-assisted supercritical carbon dioxide (USC-CO2) extraction for determining the extraction yields of oils and the contents of eugenol, β-caryophyllene, eugenyl acetate and α-humulene from clove buds. Compared to traditional SC-CO2 extraction, USC-CO2 extraction might provide a 13.5% increase in the extraction yield for the oil while utilizing less severe operating parameters, such as temperature, pressure, CO2 flow rate and the time consumed by the process.

Development of a hyphenated procedure of heat-reflux and ultrasound-assisted extraction followed by RP-HPLC separation for the determination of three flavonoids content in Scutellaria barbata D. Don.

A hyphenated procedure of heat-reflux and ultrasound-assisted extraction (HUAE), and an accurate high-performance liquid chromatographic (HPLC) method were developed for the determination of apigenin, baicalin and luteolin content in Scutellaria barbara D. Don. The suitable HUAE conditions for the extraction of target compounds from the herb were identified as an ultrasonic frequency of 40kHz, power of 185W, duty cycle of 75% (intermittent sonication), mean particle size of 0.

Optimisation of an ultrasound-assisted extraction followed by RP-HPLC separation for the simultaneous determination of oleanolic acid, ursolic acid and oridonin content in Rabdosia rubescens.

Introduction: Rabdosia rubescens is a commonly used herb in traditional Chinese medicine and contains diterpenoids, triterpenoids and various other compounds. Among these components, oridonin, oleanolic acid and ursolic acid have gained considerable interest concerning anti-cancer activities. However, there is no suitable currently available method for the simultaneous evaluation of these three bioactive compounds in R.

B1, a novel topoisomerase II inhibitor, induces apoptosis and cell cycle G1 arrest in lung adenocarcinoma A549 cells.

In our previous studies, we demonstrated that 2,6-bis-(2-chloroacetamido) anthraquinone (B1) showed a highly significant cytotoxic effect. However, its influence in the cell cycle and apoptotic induction effects has not been investigated yet. Here we report the antiproliferative effect of B1, for which IC50 values were 0.

Effects of Toona sinensis leaf extract on lipolysis in differentiated 3T3-L1 adipocytes.

The effect of substances extracted from Toona sinensis leaves with 50% alcohol solution on lipolysis was investigated in cultured 3T3-L1 differentiated adipocytes. The amount of glycerol released from cells into culture medium was used to measure lipolysis activity. Glycerol release was increased by Toona sinensis leaf extract in a dose-dependent and time-dependent manner.

Enhancement of glucose uptake in 3T3-L1 adipocytes by Toona sinensis leaf extract.

The effects of substances extracted from Toona sinensis leaves, using 50% alcohol/water, on cellular [3H]-2-deoxyglucose uptake in differentiated cultured 3T3-L1 adipocytes were investigated. Following treatment of cells with 0.001, 0.