PDMS-PUA bi-directional replication technology and its applications.

Polydimethylsiloxane (PDMS) and polyurethane acrylate (PUA) are excellent pattern transfer materials. In this study, PDMS-PUA bi-directional replication technology is explored using the PDMS grating as a template, and relevant technical issues are discussed in detail. Special surface treatment and process optimization are applied to solve the problems of demolding, PDMS polymerization inhibition, and substrate flatness.

Simultaneous Determination of Amphiphobicities of Material Surface by A Dual-Indicator Headspace Gas Chromatography.

This paper reports on a new method for simultaneously determining the amphiphobicities of material surface by a dual-indicator assisted headspace gas chromatography. After adding 40 μL of both methanol-water and toluene-oil droplets on the sample surface for 20 s, the sample was turned vertically (to remove water and oil) and then placed in a headspace vial. The amount of methanol and toluene in the residual water and oil adhered to material surface was quantified using headspace gas chromatography.

[Biological characteristics of bacteriophages infecting three typic rhizobia of legume].

Bacteriophages infecting rhizobia of legume leads to a significant decrease in the number of rhizobia in soil and nodulation in legume, which finally affects nitrogen fixation and remarkably reduces crop yield. However, limited studies have focused on bacteriophages of rhizobia. In this study, three typic rhizobium strains of Bradyrhizobium diazoefficiens USDA110, Sinorhizobium sojae CCBAU05684 and Sinorhizobium meliloti USDA1002 were used as the host bacteria.

Determination of starch gelatinization temperatures by an automated headspace gas chromatography.

This paper reports on an automated temperature-programmed headspace GC technique for the determination of gelatinization temperature of starch. 1-butanol was used as a tracer and added to the starch-solution for the test. Based on measuring the GC signal of 1-butanol in the headspace with the temperature increasing, two transition points (corresponding to the onset temperature and the ending temperature of starch gelatinization) were observed by plotting the GC signal of 1-butanol vs.

[Changes of brain CT imaging of cerebral cysticercosis patients during the period of anti-cysticercus chemotherapy].

Objective: To investigate the changes of brain CT imaging in patients with cerebral cysticercosis during the period of anti-cysticercus chemotherapy.

Methods: A total of 380 inpatients that were conformed to the practical diagnostic criteria of cerebral cysticercosis were enrolled in this study in the Third Affiliated Hospital of Shandong Academy of Medical Sciences from May 2010 to May 2015. All the patients were given anti-cysticercus chemotherapy (albendazole and praziquantel).

Determination of the softening point of rosin by a simple and automated headspace gas chromatographic technique.

We report a simple and automated headspace gas chromatographic technique for the determination of the softening point of rosin. A lumpy solid of rosin is added into a headspace vial, then an automated stepwise temperature ramping and headspace sampling was performed at each temperature stage and gas chromatography measurement. By plotting the gas chromatography signal for an impurity in rosin versus the temperature, a transition point (corresponding to the rosin softening point) was determined.

A pressure-affected headspace-gas chromatography method for determining calcium carbonate content in paper sample.

The present work reports on the development of a pressure-affected based headspace (HS) analytical technique for the determination of calcium carbonate content in paper samples. By the acidification, the carbonate in the sample was converted to CO and released into the headspace of a closed vial and then measured by gas chromatography (GC). When the amount of carbonate in the sample is significant, the pressure created by the CO affects the accuracy of the method.

[Effects of manure application on the diversity of corn root endophytic bacterial communities at seedling stage in eroded Mollisols.].

In order to investigate the change of root endophytic bacterial communities under soil erosion condition, and to evaluate the response of root endophytic bacteria to manure fertilizer, we adopted the LNA-PCR clamping and 454 bar-coded pyrosequencing methods to study the corn root endophytic bacterial communities under 30 cm topsoil erosion and manure fertilization conditions. No topsoil removing (0 cm) and only chemical fertilizer treatment were used as control. A total of 37820 valid sequences of 16S rDNA were obtained, mainly distributed in 4 phyla, 35 classes, 214 genera and 782 OTUs.

[Therapeutic effect of 138 imported falciparum malaria patients].

Objectives: To summarize the clinical characteristics of imported falciparum malaria patients and the treatment, so as to provide the evidences for improving the diagnosis and treatment of the disease.

Methods: A total of 138 imported falciparum malaria patients who received the treatment in Shandong Institute of Parasitic Diseases from January 2007 to February 2013 were adopted as the observation subjects, and their clinical data were collected and analyzed.

Results: All the 138 patients were back from African countries.

[Dipylidium caninum infection in an infant: one case report].

This paper reports the diagnosis and therapy of one case of Dipylidium caninum infection in an infant.

Follicle flushing in monofollicular in vitro fertilization almost doubles the number of transferable embryos.

Follicle flushing has been proved to be ineffective in polyfollicular in vitro fertilization. To analyze the effect of flushing in monofollicular in vitro fertilization we aspirated and then flushed the follicles in 164 cycles. Total oocyte yield/aspiration was 44.

[Technique of running urethrovesical anastomosis during radical laparoscopic prostatectomy: separate application of two needles].

Objective: To study the value and feasibility of a novel method of urethrovesical anastomosis during radical laparoscopic prostatectomy.

Methods: From 2008 to 2009, 10 patients with local prostate cancer (LPC) underwent radical laparoscopic prostatectomy through the placement of two separate needles for running urethrovesical anastomosis. The first step comprised the first stitch placed in the posterior wall of anastomosis (at 4 o'clock) and then clockwise running suture from 4 to 12 o'clock position.

[Simultaneous determination of multi-organotin compounds in seawater by liquid-liquid extraction-high performance liquid chromatography-inductively coupled plasma mass spectrometry].

The hyphenated technique of high performance liquid chromatography coupled with inductively coupled plasma mass spectrometry (HPLC-ICP-MS) was applied to the simultaneous determination of five organotin compounds (trimethyltin, dibutyltin, tributyltin, diphenyltin and triphenyltin) in seawater samples. Agilent TC-C18 column was used for the separation, the mobile phase of HPLC was CH3CN : H2O : CH3COOH = 65 : 23 : 12 (phi3), 0.05% TEA, and pH value was adjusted to 3.

[Application of EITB in immunodiagnosis of cysticercosis].

Objective: To establish a sensitive and specific method EITB for the immunodiagnosis of cysticercosis.

Methods: Two-dimensional electrophoresis was used to analyze and purify the cyst fluid antigen. Western blotting using the sera of patients with cysticercosis, hydatidosis, and other heteroserum was used to screen the specific antigens.