[Toxicity reduction of human islet amyloid polypeptide by Trim-Away technique in insulinoma cells].

Human islet amyloid polypeptide (hIAPP, also known as amylin) is a co-secreting protein of insulin in human pancreatic β-cells. It is encapsulated in vesicles and secreted out of the cells with insulin. hIAPP can promote insulin secretion and regulate blood glucose homeostasis in the body under the normal physiological conditions.

Zinc chelator TPEN induces pancreatic cancer cell death through causing oxidative stress and inhibiting cell autophagy.

The essential trace element zinc (Zn) is widely required in cellular functions, and abnormal Zn homeostasis causes a variety of health problems including immunodeficiency and sensory dysfunctions. Previous studies had shown that Zn availability was also important for tumor growth and progression. The aim of the present study was to investigate the potential mechanisms of N,N,N,N-Tetrakis(2-pyridylmethyl)-ethylenediamine (TPEN) (a membrane permeable zinc chelator) induced pancreatic cancer cell death.

[Preparation of monoclonal antibodies against human Apo B₁₀₀ and establishment of a sandwich ELISA system].

Aim: To prepare the monoclonal antibodies (mAbs) against human Apo B₁₀₀ and establish a double-antibody sandwich ELISA system for human Apo B₁₀₀ detection.

Methods: The BALB/c mice were immunized with human Apo B₁₀₀ antigen. After cell fusion and screening, the hybridoma cells were cultured in serum-free medium and the supernatant was purified to obtain mAbs using protein A.

[The application of two-dimensional liquid phase chromatography to separation of total protein of Leymus chinensis shoot].

The total proteins of Leymus chinensis were extracted from eight-week-old seedlings grown in the greenhouse by TCA-acetone precipitation method. After the lysis buffer was replaced by the start buffer, proteins were fractionated along the first dimension using chromatofocusing (CF). Subsequently the fractions with pI values between 8.