Clinical application of a modified predeposit autologous red blood cell apheresis in multistage spinal fusion: a single-center retrospective study.

Purpose: This study aimed to evaluate the efficacy and safety of predeposit autologous RBC apheresis (PARA) in patients undergoing multilevel spinal fusion surgery.

Methods: A total of 112 patients from January 2020 to June 2022 were divided into two groups according to PARA: the PARA group ( = 51) and the control group ( = 61). The baseline characteristics of the patients, outcomes, transfusion cost, hospitalization cost, length of stay, complications, and changes in hemoglobin and hematocrit levels between the two groups were compared.

Intrauterine Infusion of Leukocyte-Poor Platelet-Rich Plasma Is an Effective Therapeutic Protocol for Patients with Recurrent Implantation Failure: A Retrospective Cohort Study.

Background: The clinical application of autologous leukocyte-poor platelet-rich plasma (LP-PRP) in patients with recurrent implantation failure (RIF) is rare. This retrospective observational cohort study aimed to evaluate the efficacy of LP-PRP intrauterine infusion in patients with RIF.

Methods: Patients with RIF undergoing frozen embryo transfer (FET) from January 2019 to December 2021 ( = 118) were enrolled, with those undergoing LP-PRP intrauterine infusion as the PRP group ( = 64), and those receiving no LP-PRP treatment as the control group ( = 54).

A novel RHD allele, with c.491A > T (p.Asp164Val) mutation, identified via family pedigree analysis.

A Chinese Han man was confirmed to carry an RHD variation by serological tests, and exons 1 through 10 of the RHD gene were analyzed by sequence-specific primer-polymerase chain reaction. To clarify the nature of this mutation, Sanger sequencing was used and a c.491A > T mutation was identified in exon 4.

Flagellin attenuates experimental sepsis in a macrophage-dependent manner.

Background: Sepsis is the leading cause of death among critically ill patients, and no specific therapeutic agent is currently approved for the treatment of sepsis.

Methods: We assessed the effects of flagellin administration on survival, bacterial burden, and tissue injury after sepsis. In addition, we examined the effects on phagocytosis and bacterial killing in monocytes/macrophages.

Diagnosis of mesothelioma with deep learning.

Malignant mesothelioma (MM) is a rare but aggressive cancer. The definitive diagnosis of MM is critical for effective treatment and has important medicolegal significance. However, the definitive diagnosis of MM is challenging due to its composite epithelial/mesenchymal pattern.

CRISPR/Cas9-mediated reversibly immortalized mouse bone marrow stromal stem cells (BMSCs) retain multipotent features of mesenchymal stem cells (MSCs).

Mesenchymal stem cells (MSCs) are multipotent non-hematopoietic progenitor cells that can undergo self-renewal and differentiate into multi-lineages. Bone marrow stromal stem cells (BMSCs) represent one of the most commonly-used MSCs. In order to overcome the technical challenge of maintaining primary BMSCs in long-term culture, here we seek to establish reversibly immortalized mouse BMSCs (imBMSCs).

Elevated production of IL-36α in chronic hepatitis B virus-infected patients correlates with viral load.

Chronic hepatitis B (CHB) infection is a typical inflammatory disease characterized by a dysregulated expression of cytokines, which contributes to the pathogenesis of chronic Hepatitis B virus (HBV) infection. IL-36 cytokines (IL-36α, IL-36β, IL-36γ and IL-36Ra) are important players in infection and immunity. However, their roles in the pathogenesis of chronic HBV infection remain unknown.

Serum progranulin levels are elevated in patients with chronic hepatitis B virus infection, reflecting viral load.

Progranulin (PGRN) is implicated in infection, immunity and host defense, but its role in the pathogenesis of HBV infection remains unknown. Here we investigated whether there is dysregulated production and the clinical significance of circulating PGRN in patients with chronic HBV infection. Serum concentrations of PGRN were analyzed by enzyme-linked immunosorbent assay.

Therapeutic potential of umbilical cord mesenchymal stem cells in mice with acute hepatic failure.

Backround/objective: Mesenchymal stem cells are probably one of the most promising alternatives for liver regeneration and repair. We present data supporting the ability of human umbilical cord mesenchymal stem cells (hUCMSCs) to generate hepatic elements and discuss the best transplantation pathway.

Methods: AHF mice were given hUCMSCs through tail-vein injection or into the liver lobes.

[Study on drug resistance and molecular epidemiology of Streptococcus pneumoniae isolated in Chongqing].

Objective: To investigate the prevalence and drug resistance of Streptococcus (S.) pneumoniae in patients infected in communities and molecular epidemiology with BOX-polymerase chain reaction (PCR) in Chongqing areas.

Methods: A total of 680 clinical specimens from sputum and throat/nasal swabs were collected from patients seen from September 2000 to March 2001.

Piperacillin-sulbactam versus piperacillin-tazobactam: a multicentre, randomised, single-blind, controlled clinical trial.

The objective of this study was to compare the efficacy and safety of piperacillin-sulbactam (PIP-SBT) and piperacillin-tazobactam (PIP-TAZ) in the treatment of bacterial respiratory and urinary tract infections. A randomised, single-blind, controlled clinical trial was performed. Differences in clinical efficacy, bacteriology and safety between the two groups were subjected to statistical analysis, including intent-to-treat (ITT) analysis.