Cryptochromes (CRYs) in pepper: Genome-wide identification, evolution and functional analysis of the negative role of CaCRY1 under Phytophthora capsici infection.

Cryptochromes (CRYs) are ultraviolet-A (UV-A) and blue light photoreceptors that perceive UV-A and blue light to mediate a range of physiological processes including disease response in plants. However, there has been no report about the roles of CRY genes in pepper, which often suffers from Phytophthora blight caused by Phytophthora capsici. In this work, three pepper CRY genes were identified and their characteristics were examined by bioinformatics analysis.

Effects of compassion fatigue, structural empowerment, and psychological empowerment on the caring behaviours of intensive care unit nurses in China: A structural equation modelling analysis.

Background: Caring behaviours play a pivotal role in nursing. The negative correlation between caring behaviours and compassion fatigue in the intensive care unit (ICU) has been extensively researched. Nevertheless, considerable gaps persist in comprehending the associations of psychological empowerment and structural empowerment with outcomes, particularly in the highly stressful environment of the ICU.

Differences in Ankle Neuromuscular Control Between the Preferred Speed and Fixed Speeds During Walking.

Walking at the preferred speed, considered as a self-optimized gait pattern, is associated with improved energy conservation and cognitive abilities. However, the neuromuscular mechanisms underlying the benefits of the preferred walking speed remain unclear. Therefore, this study aimed to determine the differences in ankle neuromuscular control between the preferred and fixed speeds during walking.

Adapter-Enhanced Hierarchical Cross-Modal Pre-training for Lightweight Medical Report Generation.

Automatic medical report generation is an emerging field that aims to transform medical images into descriptive, clinically relevant narratives, potentially reducing the workload for radiologists significantly. Despite substantial progress, the increasing model parameter size and corresponding marginal performance gains have limited further development and application. To address this challenge, we introduce an Adapter-enhanced Hierarchical cross-modal Pre-training (AHP) strategy for lightweight medical report generation.

Deciphering the value of anoikis-related genes in prognosis, immune microenvironment, and drug sensitivity of laryngeal squamous cell carcinoma.

Background: Laryngeal squamous cell carcinoma (LSCC) is a prevalent malignancy characterized by high metastatic potential and poor prognosis. Anoikis, an apoptotic pathway triggered by detachment from the extracellular matrix (ECM), acts as a barrier against cancer metastasis, so it is necessary to explore the role of anoikis-related genes (ARGs) in LSCC.

Methods: Multivariate Cox regression analysis was used to construct prognostic model.

Risk of acute renal failure associated with combined use of SGLT2 inhibitors and potentially nephrotoxic drugs: an epidemiological surveillance study based on the FDA adverse event reporting system (FAERS).

Background: Limited knowledge exists regarding nephrotoxic risks associated with the combination of sodium-glucose co-transporter-2 inhibitors (SGLT2i) and potentially nephrotoxic drugs. This study evaluates acute renal failure (ARF) events linked to such combinations using data from the FDA Adverse Event Reporting System (FAERS).

Methods: Signal mining was performed by estimating reporting odds ratios (ROR), with validation through additive, multiplicative, and proportional reporting ratio (PRR) models.

Fixed-Duration Acalabrutinib Combinations in Untreated Chronic Lymphocytic Leukemia.

Background: Whether fixed-duration acalabrutinib-venetoclax (with or without obinutuzumab) would result in better progression-free survival than chemoimmunotherapy in patients with untreated chronic lymphocytic leukemia (CLL) is unknown.

Methods: In this phase 3, open-label trial, we included patients 18 years of age or older who had an Eastern Cooperative Oncology Group performance-status score of 0 to 2 (range, 0 to 5, with higher numbers indicating greater disability) and who did not have a 17p deletion or mutation. Patients were randomly assigned, in a 1:1:1 ratio, to receive acalabrutinib-venetoclax (acalabrutinib, cycles 1 to 14; venetoclax, cycles 3 to 14), acalabrutinib-venetoclax-obinutuzumab (as above, plus obinutuzumab, cycles 2 to 7), or chemoimmunotherapy with the investigator's choice of fludarabine-cyclophosphamide-rituximab or bendamustine-rituximab (cycles 1 to 6).

Hyperuricemia Exacerbates Experimental Periodontitis via Uric Acid-Induced Periodontal Inflammation and Oxidative Stress.

Aim: To investigate the effects of hyperuricemia on periodontitis and the underlying mechanisms by establishing combined animal and cell models.

Methods: A hyperuricemia mouse model was established by potassium oxonate injection, with sodium carboxymethylcellulose treatment serving as controls. Both models were treated with or without periodontitis induction (n = 10/group).

Case report: The value of early application of mNGS technology in the diagnosis and treatment of severe Legionnaires' disease: reports of two cases with different outcomes.

Background: Legionnaires' disease has a high clinical mortality rate, and early diagnosis and treatment are critical. Increasing evidence shows that metagenomic next-generation sequencing (mNGS) has excellent potential for the early identification of pathogens. To help clinicians better recognize Legionnaires' disease in its early stage and to illustrate the diagnostic value of mNGS technology, we reviewed and summarized two cases of severe Legionnaires' disease.

Excessive screen time and problem behaviours among school-age children in Fujian, China: a cross-sectional study.

Background: Screen time encompasses activities conducted on digital devices, including traditional devices such as televisions and computers, as well as modern devices like smartphones, tablets, and other digital screens. Excessive screen time among children has been linked to a heightened likelihood of engaging in high-risk problem behaviours. This study aimed to quantify the prevalence of excessive screen time and delve into its correlation with problem behaviours among school-aged children in Fujian, thereby gaining insight into the prevalence and trends within this region.

Identification of VcRBOH genes in blueberry and functional characterization of VcRBOHF in plant defense.

Reactive oxygen species (ROS) serve as signal molecules in plant defense responses, and the respiratory burst oxidase homolog (RBOH) enzyme plays a crucial role in their production. Although numerous RBOH family members have been identified in various plants, little is known about the RBOH genes in blueberries. In this study, we identified six VcRBOH genes from the blueberry genome.

Comparative efficacy of pharmacological interventions on metabolic and hormonal outcomes in polycystic ovary syndrome: a Network Meta-Analysis of Randomized controlled trials.

Background: Polycystic ovary syndrome (PCOS) is a common endocrine disorder associated with metabolic and hormonal abnormalities. This study aimed to evaluate the comparative efficacy of pharmacological interventions on these outcomes.

Methods: We conducted a systematic review and network meta-analysis of randomized controlled trials (RCTs) assessing pharmacological treatments for PCOS.

[Heat-sensitive moxibustion assisted in palliative treatment to improve the quality of life in elderly patients with malignant tumor: a randomized controlled trial].

Objective: To observe the effect of heat-sensitive moxibustion on the quality of life in the elderly patients with malignant tumor based on palliative treatment.

Methods: A total of 100 elderly patients with malignant tumor were randomly divided into an observation group (50 cases, 3 cases dropped out) and a control group (50 cases, 4 cases dropped out). The conventional palliative treatment was performed in the control group.

[Clinical efficacy of antagonistic needling therapy on post-stroke lower limb spasticity and its effect on muscle morphology].

Objective: To observe the effects of antagonistic needling therapy on lower limb spasticity and the muscle morphology of the tibialis anterior and gastrocnemius in patients with stroke.

Methods: A total of 100 patients with post-stroke lower limb spasticity were randomly divided into an antagonistic needling group (50 cases, 1 case dropped out) and a routine acupuncture group (50 cases, 1 case dropped out). Both groups received basic treatment and rehabilitation training.

Development of Recombinant Human Collagen-Based Porous Scaffolds for Skin Tissue Engineering: Enhanced Mechanical Strength and Biocompatibility.

Skin tissue engineering scaffolds should possess key properties such as porosity, degradability, durability, and biocompatibility to effectively facilitate skin cell adhesion and growth. In this study, recombinant human collagen (RHC) was used to fabricate porous scaffolds via freeze-drying, offering an alternative to animal-derived collagen where bovine collagen (BC)-based scaffolds were also prepared for comparison. The internal morphology of the RHC scaffolds were characterized by scanning electron microscopy (SEM) and the pore size ranged from 68.

Conserved GTPase OLA1 promotes efficient translation on D/E-rich mRNA.

The TRAFAC (translation factors) GTPase OLA1 plays a critical role in various stress responses and is implicated in the regulation of tumor progression. It is conserved from bacteria to eukaryotes and regulates the translation through binding to the ribosome. Here, we report the cryo-electron microscopy structure of its Escherichia coli homolog, YchF, with the 50S subunit.

Smart Pharmaceutical Monitoring System With Personalized Medication Schedules and Self-Management Programs for Patients With Diabetes: Development and Evaluation Study.

Background: With the climbing incidence of type 2 diabetes, the health care system is under pressure to manage patients with this condition properly. Particularly, pharmacological therapy constitutes the most fundamental means of controlling blood glucose levels and preventing the progression of complications. However, its effectiveness is often hindered by factors such as treatment complexity, polypharmacy, and poor patient adherence.

Identification of key genes underlying radiosensitivity and radioresistance in endometrial cancer through integrated bioinformatics analysis.

Introduction: Radiation therapy is crucial in the treatment of endometrial cancer (UCEC). Patients exhibit significant variability in radiosensitivity, affecting therapeutic effect. Scarcity of studies exploring the gene-radiosensitivity relationship based on clinical data.

Quantification of mycophenolic acid in plasma by isotope dilution liquid chromatography-tandem mass spectrometry candidate reference method.

Accurate measurements of plasma mycophenolic acid (MPA) are essential for therapeutic drug monitoring in transplant recipients and autoimmune diseases. The performance of plasma mycophenolic acid routine methods remains highly variable that calls for a candidate reference measurement procedure (cRMP) to improve the standardization of plasma mycophenolic acid measurements. In this study, sample preparation was based on protein precipitation with methanol followed by further dilution.

Mechanistic insights into the role of traditional Chinese medicine in treating gastric cancer.

Gastric cancer remains a leading cause of cancer-related mortality worldwide, with advanced stages presenting significant challenges due to metastasis and drug resistance. Traditional Chinese Medicine (TCM) offers a promising complementary approach characterized by holistic treatment principles and minimal side effects. This review comprehensively explores the multifaceted mechanisms by which TCM addresses gastric cancer.

Discovery and Optimization of a Series of Novel Morpholine-Containing USP1 Inhibitors.

Ubiquitin-specific protease 1 (USP1), a well-known member of the deubiquitinating enzymes, serves as a key regulator in DNA damage repair (DDR) processes. Herein, we utilized ring-opening and cyclization strategies based on KSQ-4279 to design a novel series of USP1 inhibitors featuring a morpholine scaffold. Notably, compound exhibited a more potent enzymatic and cellular inhibition activity compared to KSQ-4279.

Enantioselective Synthesis of Indole-Fused Polycycles Bearing Four Consecutive Stereocenters via Rhodium Catalysis.

Indole-fused polycycles are common in natural products and bioactive molecules, yet their concise and efficient synthesis remains challenging, especially for compounds with multiple stereocenters. Herein, we report the application of a chiral CpRh catalyst in the enantioselective C-H activation/[4+2] annulation of indoles with bicyclic alkenes. This chiral catalytic system exhibits high enantioselectivity and broad functional group tolerance and operates under benign conditions.

Strengthening perovskite interfaces with in-situ polymerized self-assembled monolayers.

Poly(3,4-ethylenedioxythiophene)-poly(styrene sulfonate) (PEDOT:PSS) has been widely used as the hole transport layers (HTLs) for perovskite solar cells (PSCs), especially in all-perovskite tandems. However, the energy-level mismatch between PEDOT:PSS and perovskite leads to large voltage deficit in PSCs, and the dopant PSS with high acidity and hygroscopicity conspicuously deteriorates the device stability. Herein, a powerful strategy for constructing self-assembled polymer HTLs is developed by in-situ polymerization of functionalized 3,4-ethylenedioxythiophene with carboxylic acids as side groups.

Multifunctional Fluorescent Probe for Simultaneous Detection of ATP, Cys, Hcy, and GSH: Advancing Insights into Epilepsy and Liver Injury.

Adenosine triphosphate (ATP) is a critical intracellular energy currency that plays a key role in various cellular processes and is closely associated with numerous diseases. Similarly, biothiols such as glutathione (GSH), cysteine (Cys), and homocysteine (Hcy) are integral to many physiological and pathological processes due to their strong redox properties. Simultaneous discrimination and detection of ATP and biothiols offer valuable insights into the pathogenesis of conditions such as epilepsy and liver injury.