Freezing-driven reduction of chromium(Ⅵ) in ice under solar light without external reductants.

This study systematically investigated the direct photoreduction of Cr(Ⅵ) in ice under simulated sunlight without external reductants. It was shown that the Cr(Ⅵ) photoreduction was observed only in ice at near-neutral pH values but not in aqueous solutions. Cr(Ⅵ) at an initial concentration of 10 μM was efficiently reduced by 75.

Prediction of clinical pregnancy outcome after single fresh blastocyst transfer during in vitro fertilization: an ensemble learning perspective.

To establish a predictive model for clinical pregnancy outcomes following the transfer of a single fresh blastocyst in vitro fertilization (IVF). 615 patients (492 in training set and 123 in test set) who underwent the first single and fresh blastocyst transfer in the first IVF or intracytoplasmic sperm injection cycle performed in fertility centre of Shenzhen Zhongshan Obstetrics & Gynecology Hospital from July 2015 to June 2021 were enrolled in this study. Conventional method such as logistic regression (LR), individual machine learning methods including naive bayesian (NB), K-nearest neighbours (K-NN), support vector machine (SVM), decision tree (DT), and ensemble learning methods including random forest (RF), XGBoost, LightGBM, Voting were used to establish the clinical pregnancy outcome prediction model, and the efficacy among different models was compared.

Programming DNA Nanoassemblies into Polyvalent Lysosomal Degraders for Potent Degradation of Pathogenic Membrane Proteins.

Lysosome-targeting chimera (LYTAC) shows great promise for protein-based therapeutics by targeted degradation of disease-associated membrane or extracellular proteins, yet its efficiency is constrained by the limited binding affinity between LYTAC reagents and designated proteins. Here, we established a programmable and multivalent LYTAC system by tandem assembly of DNA into a high-affinity protein degrader, a heterodimer aptamer nanostructure targeting both pathogenic membrane protein and lysosome-targeting receptor (insulin-like growth factor 2 receptor, IGF2R) with adjustable spatial distribution or organization pattern. The DNA-based multivalent LYTACs showed enhanced efficacy in removing immune-checkpoint protein programmable death-ligand 1 (PD-L1) and vascular endothelial growth factor receptor 2 (VEGFR2) in tumor cell membrane that respectively motivated a significant increase in T cell activity and a potent effect on cancer cell growth inhibition.

Programming Fast DNA Amplifier Circuits with Versatile Toehold Exchange Pathway.

DNA amplifier circuits establish powerful tools to dynamically control molecular assembly for computation, sensing, and biological applications. However, the slow reaction speed remains a major barrier to their practical utility. Here, diverse fast DNA amplifier circuits termed toehold exchange polymerization (TEP) and toehold exchange catalysis (TEC) using toehold exchange-mediated assembly as a fundamental mechanism are built.

Endometrial preparation protocols did not impact pregnancy outcomes of patients with cured chronic endometritis.

Research Question: Do endometrial preparation protocols have an effect on pregnancy outcomes in patients with cured chronic endometritis?

Design: A retrospective study was conducted on 3721 infertile patients from December 2018 to August 2020. Endometrial tissues obtained during the proliferative phase were immunostained for CD138. The presence of CD138-positive cells within the stromal cells indicated chronic endometritis.

Inhibition of Alzheimer's disease by 4-octyl itaconate revealed by RNA-seq transcriptome analysis.

Aims: This study aimed to examine the therapeutic effects and response mechanisms of 4-OI in Alzheimer's disease (AD).

Methods: In this study, network pharmacology was employed to analyze potential targets for AD drug therapy. Immunofluorescence and quantitative reverse transcription polymerase chain reaction (qRT-PCR) techniques were utilized to detect inflammatory phenotypes in a 4-OI-resistant mouse microglia cell line (BV2).

Attenuated memory impairment and neuroinflammation in Alzheimer's disease by aucubin via the inhibition of ERK-FOS axis.

Alzheimer's disease (AD) is a degenerative illness accompanied by cognitive and memory loss. In addition to the widely accepted, convincing amyloid cascade hypothesis, the activation of glial cells and neuroinflammation, especially the microglia-mediated neuroinflammation, has an essential role in the development and progression of AD. Therefore, the anti-inflammatory treatment is becoming a promising therapeutic strategy.

Platform for Quantitative Detection of Endometrial Immune Cells Based on Immunohistochemistry and Digital Image Analysis.

To evaluate the endometrial immune microenvironment of patients with recurrent miscarriage (RM), a digital immunohistochemistry image analysis platform was developed and validated to quantitatively analyze endometrial immune cells during the mid-luteal phase. All endometrium samples were collected during the mid-luteal phase of the menstrual cycle. Paraffin-embedded endometrial tissues were sectioned into 4 µm thick slides, and immunohistochemistry (IHC)staining was carried out for detecting endometrial immune cells, including CD56+ uNK cells, Foxp3+ Tregs, CD163+ M2 macrophages, CD1a+ DCs, and CD8+ T cells.

A self-controlled, cross-over study of intensive insulin treatment with needle-based injection versus needle-free injection in hospitalized patients with type 2 diabetes.

Background And Aims: Needle injection and needle-free injection were proven effective in improving glycated hemoglobin (HbA1c) in type 2 diabetes mellitus (T2DM) patients. However, it is unclear if needle-free and needle injections of insulin during intensive insulin therapy in hospitalized patients provide similar efficacy and safety benefits.

Methods: A self-controlled cross-over study was conducted on 62 patients with T2DM who received intensive long-acting and short-acting insulin injections with or without needles.

Comparing the peri-implantation endometrial T-bet/GATA3 ratio between control fertile women and patients with recurrent miscarriage: establishment and application of a reference range.

Study Question: Is the ratio of endometrial T-box expressed in T cell (T-bet) and GATA-binding protein 3 (GATA3) changed in patients with recurrent miscarriage (RM) compared to fertile controls?

Summary Answer: Our study showed a significantly higher T-bet/GATA3 ratio in patients with RM compared with fertile controls.

What Is Known Already: The endometrial T-bet (Th1 lineage-committed transcription factor)/GATA3 (Th2 lineage-committed transcription factor) ratio could represent the Th1/Th2 balance, which is particularly important for healthy pregnancy. However, a reliable reference range for the T-bet/GATA3 ratio during the peri-implantation period has not yet been established for use in clinical practice.

Modeling the dynamic changes in Plasmopara viticola sporangia concentration based on LSTM and understanding the impact of relative factor variability.

Reliable disease management can guarantee healthy plant production and relies on the knowledge of pathogen prevalence. Modeling the dynamic changes in spore concentration is available for realizing this purpose. We present a novel model based on a time-series modeling machine learning method, i.

Exosomes-delivered PD-L1 siRNA and CTLA-4 siRNA protect against growth and tumor immune escape in colorectal cancer.

Objective: This study aims to dissect impacts of exosomes-delivered PD-L1 and CTLA-4 siRNAs on colorectal cancer (CRC) progression and immune responses.

Methods: Exosomes containing PD-L1 siRNA and CTLA-4 siRNA were prepared and utilized to treat CRC cells to evaluate their effects. A tumor-bearing mouse model was established for verification.

Can precancerous stem cells be risk markers for malignant transformation in the oral mucosa?

Accurate assessment of the carcinogenic potential of oral mucosal diseases can significantly reduce the prevalence of oral cancer. We speculate that precancerous stem cells (pCSCs) arise during the evolution of carcinomas based on long-term experimental findings, published literature, and the cancer stem cell (CSC) theory, wherein pCSCs exist in precancerous lesions and have characteristics of both CSCs and normal stem cells. This apparently contradictory feature may be the foundation of the reversible transformation of precancerous lesions.

The mechanisms underlying the enrichment and action of glypican-1-positive exosomes in colorectal cancer cells.

Background: Glypican-1 (GPC1) is overexpressed in several tumors, and GPC1 exosomes have shown the potential to predict early colorectal cancer (CRC). However, the mechanisms underlying the enrichment and action of GPC1 exosomes in CRC remain unknown.

Methods: The expression of slit guidance ligand 2 (SLIT2), hypoxia-inducible factor (HIF)-1α/2α, and GPC1 in clinical CRC tissues was detected using immunohistochemistry and western blot.

Impact of body mass index on peripheral and uterine immune status in the window of implantation in patients with recurrent reproductive failure.

This study aimed to investigate whether maternal obesity affects the immune status of peripheral blood and endometrium in patients with recurrent reproductive failure classified according to their body mass index (BMI). A total of 228 repeated implantation failure (RIF) and 266 recurrent miscarriage (RM) patients were enrolled in the study and further subdivided into three groups according to their BMI: (i) normal weight (18.5≤ BMI <23); (ii) overweight (23≤ BMI <25); and (iii) obese (BMI ≥25).

Polypropylene microplastics aging under natural conditions in winter and summer and its effects on the sorption and desorption of nonylphenol.

Plastics in the environment undergo various aging effects. Due to the changes in physical and chemical properties, the sorption behavior of aged microplastics (MPs) for pollutants differs from that of pristine MPs. In this paper, the most common disposable polypropylene (PP) rice box was used as the source of MPs to study the sorption and desorption behavior of nonylphenol (NP) on pristine and naturally aged PPs in summer and winter.

Genome Resource of PY-1, a Broad-Spectrum Antimicrobial Strain in Particular Antagonistic Against .

PY-1 exhibited promising antimicrobial properties; in particular, it is highly inhibitory to , which causes downy mildew of grape. It is very necessary to carry out systematic and in-depth research on the PY-1 strain for the improvement, application, and promotion of biocontrol agents. The PY-1 genome was fully sequenced and assembled.

Establishment of reference intervals of endometrial immune cells during the mid-luteal phase.

This study aimed to develop reference intervals (RIs) of endometrial immune cells in control infertile women during the mid-luteal phase, and compare with the proportion of endometrial immune cells in recurrent reproductive failure (RRF) patients. Endometrial tissue sections were obtained from 113 fertile women and 79 patients with RRF, including 40 patients who had suffered recurrent miscarriage (RM) and 39 patients with repeated implantation failure (RIF) during the mid-luteal phase of the menstrual cycle. Immunohistochemical staining and quantitative analysis of CD56, Foxp3, CD163, CD1a and CD8 cells were performed in endometriums.

The protective effects of dietary Clostridium butyricum supplementation on hepatic ischemia reperfusion injury in rats.

Purpose: This study investigated the effects of oral administration of Clostridium butyricum (C. butyricum) on inflammation, oxidative stress, and gut flora in rats with hepatic ischemia reperfusion injury (HIRI).

Methods: The rats from C.

Chronic endometritis impairs embryo implantation in patients with repeated implantation failure: A retrospective study.

Objective: To assess whether chronic endometritis (CE) affects embryo implantation in patients with repeated implantation failure (RIF).

Materials And Methods: We retrospectively analyzed 126 RIF patients who were never diagnosed with CE and received no prior antibiotic therapy. Endometrial specimens obtained by endometrial scratching during mid-luteal phase were immunostained by CD138, a hallmark plasmacyte marker, to identify CE.

Screening, and Optimization of Fermentation Medium to Produce Secondary Metabolites from for the Biocontrol of Early Leaf Spot Disease, and Growth Promoting Effects on Peanut ( L.).

A novel Bacillus amyloliquefaciens BAM strain, with novel fermentation nutrient mediums and compositions, could produce potent antifungal secondary metabolites, as the existing strains face resistance from fungus pathogens. In the current study, we introduced two novel nutrient mediums for the fermentation process, semolina and peanut root extract, as carbon and nitrogen sources in order to maximize the antifungal effects of B. amyloliquefaciens against Cercaspora arachidichola to control early leaf spot disease in peanuts.

A Comparison of Three Types of "Vineyard Management" and Their Effects on the Structure of Populations and Epidemic Dynamics of Grape Downy Mildew.

Grape downy mildew (GDM) is a destructive grapevine disease caused by that occurs worldwide. In this study, we determined the characteristics of GDM epidemics and the grapevine canopy micro-climate in open-field, fungicide-spray, and rain-shelter plots during two constitutive years (2016 and 2017). It was found that rain shelter can significantly delay the disease occurrence by 28 and 21 days, reduce the epidemic phase by 28 and 21 days, and decrease the final disease index by 82% and 83%.

Adsorption of acetone, ethyl acetate and toluene by beta zeolite/diatomite composites: preparation, characterization and adsorbability.

The hierarchical porous composites (Beta/Dt) were prepared by secondary growth method using natural diatomite and beta zeolite. Moreover, XRD, SEM, and BET characterize the composite's composition, surface structure, and pore structure. The adsorbability of Beta/Dt was evaluated by adsorption of three common volatile organic compounds (VOCs) of the printing industry: acetone, ethyl acetate, and toluene.

Unveiling the relationship between the multilayer structure of metallic MoS and the cycling performance for lithium ion batteries.

Molybdenum disulfide (MoS) with a layered structure is a desirable substitute for the graphite anode in lithium ion storage. Compared with the semiconducting phase (2H-MoS), the metallic polymorph (1T-MoS) usually shows much better cycling stability. Nevertheless, the origin of this remarkable cycling stability is still ambiguous, hindering further development of MoS-based anodes.