[ERYTHROKARIOCYTES APOPTO- TIC ACTIVITY IN ERYTHROBLASTIC ISLANDS OF RATS BONE MARROW].

The functional activity of bcl-2 andp53 proteins in erythrokariocytes of erythroblastic islands (EI) of various classes of bone marrow belonging to intact rats and animals following an acute blood loss were investigated. It is observed that intact rats had the elevated levels of percent content of bcl-2+ erythrokariocytes in EI of the second maturity class, compared to EI of first maturity class and EI involutionary class, and also less in the EI involutionary class compared to EI 1 and EI 2. The percentage content of p53+ erythrokariocytes in intact rats in the EI 2 class was less than in EI 1 and EI involutionary class, and were nearly the same of EI 1, EI 3, EI involutionary class and EI reconstructed.

[REGULATION OF EXTRARENAL ERYTHROPOIETIN PRODUCTION].

The review describes the induction of erythropoietin gene expression in liver, reproouctive and hemopoietic systems during hypoxia or a state of proliferation.

[INFLUENCE OF TOTAL RNA SPLENIC LYMPHOID CELLS ON ERYTHROPOIESIS IN EXPERIMENTAL POLYCYTHEMIA].

Total RNA, isolated from rat spleen's lymphoid cells during the period of hematopoietic tissue's reparative regeneration, has a remarkable activating effect on the processes of erythroblastic islet formation in vivo and in vitro. Total RNA, isolated from normal rat spleen's lymphoid cells, restores depressed erythropoiesis in erythroblastic islets up to physiological level.

Effects of fullerenol C₆₀(OH)₂₄ on erythropoiesis in vitro.

The effects of fullerenol C60(OH)24 in doses of 0.1-100 μg/ml on erythropoiesis were studied in the culture of erythroblastic islets of the bone marrow. Fullerenol in concentrations of 10 and 100 μg/ml had negative effects on the development of erythroid tissue: it inhibits proliferation of erythroid cells, delays erythroblast maturation, decelerates recruitment of erythroid CFU to differentiation, and suppresses repeated involvement of macrophages in erythroblastic islets.