In vivo Bidirectional Modulation of Cannabinoid on the Activity of Globus Pallidus in Rats.

Endocannabinoids are bioactive substances which participate in central motor control. The globus pallidus (GP) is a major nucleus in the basal ganglia circuit, which plays an important function in movement regulation. Both cannabinoid receptor type 1 (CBR) and cannabinoid receptor type 2 (CBR) are expressed in the GP suggesting GP as a main action area of endocannabinoids.

Tumor suppressor miR-192-5p targets TRPM7 and inhibits proliferation and invasion in cervical cancer.

Cervical cancer is the fourth highest mortality cancer among women worldwide. Many researchers have discovered the major anticancer role of miR-192-5p. However, no study has revealed the effect of miR-192-5p on cervical cancer and its molecular mechanism.

Consistency of postoperative pain assessments between nurses and patients undergoing enhanced recovery after gynaecological surgery.

Aims And Objectives: To explore the consistency of pain intensity and pain location assessed by nurses and patients in gynaecology undergoing enhanced recovery after surgery pathway.

Background: Several studies have shown that clinical nurses' assessment of patients' pain is not always accurate. Little is known about the accuracy of nurses' pain assessments for gynaecological patients.

Anti-inflammatory effect of Yam Glycoprotein on lipopolysaccharide-induced acute lung injury via the NLRP3 and NF-κB/TLR4 signaling pathway.

Acute lung injury (ALI) is a common lung disease accompanied by acute and persistent pulmonary inflammatory response syndrome, which leads to alveolar epithelial cells and capillary endothelial cell damage. Yam glycoprotein, separated from traditional Chinese yam, has been shown to have anti-inflammatory and immunomodulatory effects. In this experiment, we mainly studied the therapeutic effect and mechanism of a glycoprotein on the lipopolysaccharide (LPS)-induced ALI mice.

Fraxin inhibits lipopolysaccharide-induced inflammatory cytokines and protects against endotoxic shock in mice.

Fraxin, the effective component isolated from Cortex Fraxini, has been reported to have anti-inflammation effects. The aim of this study was to explore the effect of fraxin on lipopolysaccharide (LPS)-induced endotoxic shock in mice. We used Kunming male mice to establish the model, and we found that fraxin could improve the survival rate of the LPS-induced mice.

TSLP promotes angiogenesis of human umbilical vein endothelial cells by strengthening the crosstalk between cervical cancer cells and eosinophils.

Our previous study demonstrated that thymic stromal lymphopoietin (TSLP) secreted by cervical cancer cells promotes angiogenesis and recruitment, and regulates the function of eosinophils (EOS). However, the function of TSLP in the crosstalk between EOS and vascular endothelial cells in cancer lesions remains unknown. The aim of the present study was to investigate the effect of EOS caused by TSLP in angiogenesis of human umbilical vein endothelial cells (HUVECs).

Protopanaxadiol and metformin synergistically inhibit estrogen-mediated proliferation and anti-autophagy effects in endometrial cancer cells.

Metformin is commonly used for treating type II diabetes and has recently been reported to possess anti-proliferative properties that can be exploited for the prevention and treatment of a variety of cancers. Ginsenosides are the main effective biological components of ginseng. It has been reported that ginsenoside-Rb2 inhibit the invasiveness of endometrial cancer cells (ECC).

Cisplatin inhibits the growth, migration and invasion of cervical cancer cells by down-regulating IL-17E/IL-17RB.

Interleukin (IL)-17E mainly produced by immune cells, is a distinct member of the IL-17 cytokine family, which has multifarious immunomodulatory activities. As a potent anticancer drug, cisplatin is commonly used against various types of solid tumors. The present study was performed to investigate whether cisplatin regulates the expression of IL-17E and it receptor IL-17RB, and the role of IL17E in cervical cancer cells .

The cross talk between cervical carcinoma cells and vascular endothelial cells mediated by IL-27 restrains angiogenesis.

Problem: To explore whether cervical carcinoma cell-derived interleukin-27 (IL-27) modulates the angiogenesis of vascular endothelial cells.

Method Of Study: The expression of IL-27 in cervical cancer tissues and cervical cell lines was analyzed by immunohistochemistry, ELISA and flow cytometry. Then, the effects of IL-27 on the proliferation and apoptosis-related molecules and angiogenesis in vitro of human umbilical vein endothelial cells (HUVECs) were investigated.

RANKL/RANK interaction promotes the growth of cervical cancer cells by strengthening the dialogue between cervical cancer cells and regulation of IL-8 secretion.

Receptor activator for nuclear factor κB ligand (RANKL) is a member of the tumor necrosis factor (TNF) family. The interaction between RANKL and its receptor RANK plays an important role in the development and function of diverse tissues. However, the expression and role of RANKL in cervical cancer are still unknown.

Enhancement of anammox performance by Cu(II), Ni(II) and Fe(III) supplementation.

This study explored the influence of metal ion addition on specific anaerobic ammonium oxidation activity (SAA). Batch assays were used to demonstrate the enhancement of the SAA upon the addition of Cu2+, Ni2+ and Fe3+. The SAA was enhanced by 41.

Hypoxia promotes the proliferation of cervical carcinoma cells through stimulating the secretion of IL-8.

To explore whether hypoxia and interleukin 8 (IL-8) regulate the viability and apoptosis of cervical carcinomas cells and the possible mechanism. We evaluated the expression of hypoxia inducible factor-1α (HIF-1α), IL-8 and its receptors (CXCR1 and CXCR2) in cervical cancer and cervicitis tissues by immunohistochemistry. Then the effects of hypoxia and IL-8 on the viability and apoptosis of HeLa and SiHa cells were detected by the SRB and apoptosis assays.

Transient and long-term effects of bicarbonate on the ANAMMOX process.

In this study, the effects of both transient and long-term inorganic carbon (IC) addition on the anaerobic ammonium oxidation (ANAMMOX) process under pseudo-steady-state and substrate inhibitions were analyzed using reactor performance and measures of sludge activity. Compared with the nitrogen removal rate (NRR) of 3.42 kg N m(-3) day(-1) in the control bioreactor (ICDR) without IC, the peak NRR reached 21.

Enhancement of ANAMMOX activity by low-intensity ultrasound irradiation at ambient temperature.

This paper aims to investigate the enhancement effect of low intensity intermittent ultrasound irradiation on the efficiency of anaerobic ammonium oxidation (ANAMMOX) process at ambient temperature. With intermittently irradiated (ultrasound intensity of 0.19 w/cm(2), exposure time of 0.

The effect of sulfide inhibition on the ANAMMOX process.

The feasibility of anaerobic ammonium oxidation (ANAMMOX) process to treat wastewaters containing sulfide was studied in this work. Serum bottles were used as experimental containers in batch tests to analyze the short-term response of the ANAMMOX process under sulfide stress. The IC(50) of sulfide-S for ANAMMOX biomass was substrates-dependent and was calculated to be 264 mg L(-1) at an initial total nitrogen level of 200 mg L(-1) (molar ratio of ammonium and nitrite was 1:1).

Hyaluronic acid derivative-based self-assembled nanoparticles for the treatment of melanoma.

Purpose: Hyaluronic acid-ceramide (HACE)-based nanoparticles (NPs) were developed for the targeted delivery of doxorubicin (DOX), and their antitumor efficacy for melanoma was evaluated.

Methods: DOX-loaded HACE-based self-assembled NPs were prepared and their physicochemical properties were characterized. The in vitro cytotoxicity of HACE was measured using an MTS-based assay.

The ANAMMOX reactor under transient-state conditions: process stability with fluctuations of the nitrogen concentration, inflow rate, pH and sodium chloride addition.

The process stability of an anaerobic ammonium oxidation (ANAMMOX) was investigated in an upflow anaerobic sludge blanket reactor subjected to overloads of 2.0- to 3.0-fold increases in substrate concentrations, inflow rates lasting 12 or 24h, extreme pH levels of 4 and 10 for 12h and a 12-h 30 g l(-1) NaCl addition.

Analysis of diferentially expressed protein from primary and recurrent ovarian cancer serum.

Objective: To study the value of the differentially expressed proteins from primary and recurrent ovarian cancer serum for early diagnosis of primary and recurrent ovarian cancer.

Methods: WCX kit (Bruker Daltonics GraBH) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) technology were used to detect serum samples from 49 patients with primary ovarian cancer and 21 patients with recurrent disease.

Results: In the mass range (Mr) from 1,000 to 12,000 Da, eight differentially expressed protein peaks were screened from primary ovarian cancer serum.

Performance and robustness of an ANAMMOX anaerobic baffled reactor subjected to transient shock loads.

The impacts of transient overloads on the performance of a laboratory-scale anaerobic ammonium oxidation (ANAMMOX) anaerobic baffled reactor was studied by increasing the substrate concentration or inflow rate to 1.5-3.0 times above normal values.

Dihydrochalcone glucosides and antioxidant activity from the roots of Anneslea fragrans var. lanceolata.

Bioassay-guided fractionation of the roots of Anneslea fragrans var. lanceolata led to the isolation of four dihydrochalcone glucosides, davidigenin-2'-O-(6″-O-4″'-hydroxybenzoyl)-β-glucoside (1), davidigenin-2'-O-(2″-O-4″'-hydroxybenzoyl)-β-glucoside (2), davidigenin-2'-O-(3″-O-4″'-hydroxybenzoyl)-β-glucoside (3), and davidigenin-2'-O-(6″-O-syringoyl)-β-glucoside (4), and 13 known compounds. The structures were identified by means of spectroscopic analysis.

Impacts of transient salinity shock loads on Anammox process performance.

The effect of salinity shock (5-60 g l(-1) NaCl) on anaerobic ammonium oxidation (Anammox) process performance was investigated. The response to the shock loads can be divided into three stages: a sensitive period, an interim stable period and a recovery period which lasted 6-26 d. When exposed to NaCl shocks for 12h, the sludge retention time (SRT) of the reactor decreased with increasing NaCl shock loads, ranging between 2.

Polyethylene glycol-conjugated hyaluronic acid-ceramide self-assembled nanoparticles for targeted delivery of doxorubicin.

Polyethylene glycol (PEG)-conjugated hyaluronic acid-ceramide (HACE) was synthesized for the preparation of doxorubicin (DOX)-loaded HACE-PEG-based nanoparticles, 160 nm in mean diameter with a negative surface charge. Greater uptake of DOX from these HACE-PEG-based nanoparticles was observed in the CD44 receptor highly expressed SCC7 cell line, compared to results from the CD44-negative cell line, NIH3T3. A strong fluorescent signal was detected in the tumor region upon intravenous injection of cyanine 5.