A novel triple signal amplification platform of peroxide test strip for sensitive detection of adenosine triphosphate.

Peroxide test strip (PTS) has been widely used for the point-of-care testing (POCT), but its poor sensitivity is a big obstacle for analyzing trace target. Herein, a triple signal amplifying platform integrating the liposome enrichment, the hybridization chain reaction (HCR) circuit, and the image analysis method was constructed to enhance the visual readout of PTS for the sensitive detection of adenosine triphosphate (ATP), an important biomarker of food spoilage. In the presence of ATP, the HCR amplifier was firstly initiated on the surface of magnetic beads (MBs), inducing a large number of glucose oxidase (GOD) imbedded liposomes to be attached.

Bifunctional In-MOFs for Selective and Sensitive Detection of Trace Nitrobenzene Compounds in Water and Possessing High Proton Conductivity.

With the escalating prevalence of terrorism and global environmental pollution, nitroaromatic compounds (NACs) have increasingly come into focus as the primary culprit. To counter these challenges, it is imperative to develop simple and efficient methods for detecting NACs. Considering the electron-deficient structure of NAC molecules, this paper constructed a novel three-dimensional In-MOF with permanent porosity using electron-rich organic molecules 4'-[1,2,2-tris(3',5'-dicarboxy[1,1'-biphenyl]-4-yl)ethenyl]-[1,1'-biphenyl]-3,5-dicarboxylic acid (HETTB) for fluorescence detection by photoinduced electron transfer.

CRISPR-Cas12a Coupled with DNA Nanosheet-Amplified Fluorescence Anisotropy for Sensitive Detection of Biomolecules.

DNA nanosheets (DNSs) have been utilized effectively as a fluorescence anisotropy (FA) amplifier for biosensing. But, their sensitivity needs to be further improved. Herein, CRISPR-Cas12a with strong -cleavage activity was utilized to enhance the FA amplification ability of DNSs for the sensitive detection of miRNA-155 (miR-155) as a proof-of-principle target.

Trait value and phenotypic integration contribute to the response of exotic Rhus typhina to heterogeneous nitrogen deposition: A comparison with native Rhus chinensis.

The temporal heterogeneity of nitrogen availability in soils is increasing due to agricultural deposition. We here compared the effects of gradually increasing nitrogen deposition rate and its increasing temporal heterogeneity patterns on the functional traits of seedlings of exotic species Rhus typhina and the native species Rhus chinensis. Nitrogen deposition rates of 0, 8, 20 g N m year and constant, single-peak, and double-peak nitrogen were added to simulate deposition rate and temporal heterogeneity.

The role of medical gas in stroke: an updated review.

Medical gas is a large class of bioactive gases used in clinical medicine and basic scientific research. At present, the role of medical gas in neuroprotection has received growing attention. Stroke is a leading cause of death and disability in adults worldwide, but current treatment is still very limited.

Induction of Antiphytopathogenic Metabolite and Squalene Production and Phytotoxin Elimination by Adjustment of the Mode of Fermentation in Cocultures of Phytopathogenic and .

The investigation of the metabolites from different cocultures of and in solid medium revealed two new squalenes ( and ); one new azaphilone (); two new tremulane sesquiterpenes ( and ); and three known compounds, conocenol B (), conocenol C (), and 4-(4-dihydroxymethylphenoxy)benzaldehyde (). The antagonistic relationship was examined by studying metabolite production. The production of compounds and by after the induction of coculture indicated significant selectivity for antifungal activity against phytopathogenic , with MICs of 16 μg/mL; compounds and also exhibited antifungal activities in vivo against infected by at concentrations of 100 μg/mL.

Assessment on the binding characteristics of dasatinib, a tyrosine kinase inhibitor to calf thymus DNA: insights from multi-spectroscopic methodologies and molecular docking as well as DFT calculation.

The binding characteristics of calf thymus DNA (ct-DNA) with dasatinib (DSTN), a tyrosine kinase inhibitor was assessed through multi-spectroscopic methodologies and viscosity measurement combined with molecular docking as well as DFT calculation to understand the binding mechanism, affinity of DSTN onto ct-DNA, effect of DSTN on ct-DNA conformation, and among others. The results confirmed DSTN bound onto ct-DNA, leading to forming the DSTN-ct-DNA complex with the binding constant of 4.82 × 10 M at 310 K.

[Overexpression of miR-30a Promotes Apoptosis of Leukemia K562 Cells].

Objective: To investigate the pro-apoptotic effect and mechanism of miR-30a overexpression on chronic myeloid leukemia K562 cells.

Methods: The k562 cells were transfected with the recombinant plasmid pEGFP-pre-miR-30a, the real-time quantitative PCR was used to detect the level of miR-30a and BCR/ABL, and then the cell apoptosis was assessed by flow cytometry with AnnexinV-FITC/PI double staining. Western blot was used to detect the expression of BCR/ABL protein,apoptosis-related protein BCL-2 and BAX, PTEN, AKT and p-AKT.