Effect of hydroxyapatite as a component of biostable composites on population and proliferation of mesenchymal stem cells.

Three groups of biostable composite materials were studied. The initial binder polymers (polymethylmethacrylate, polyamide-12, superhigh-molecular-weight polyethylene) and hydroxyapatite-containing composites on the basis of these polymers were tested. Biostable polymers, including those containing hydroxyapatite, were nontoxic for fibroblasts and mesenchymal stem cells: the adhesion parameters for these cells were maximum for polyamide-12 and superhigh-molecular-weight polyethylene and did not depend on the presence of hydroxyapatite.

Structure of human vertebral lamellar bone in age-associated involution and osteoporosis.

We compared the structure of lamellar bone in anterolateral zones of vertebral bodies in adult and senile subjects in health and osteoporosis. The appearance of structural signs of age-related osteopenia and enlargement of coplanar consolidations of the bone matrix crystals were noted. Osteopenia was more pronounced in osteoporosis, while the structural organization of the mineral component did not differ from that in age-matched controls.

Comparison of cytotoxicity of aminoglycoside antibiotics using a panel cellular biotest system.

The cytotoxicity of four aminoglycoside antibiotics was studied by estimation of the dose-effect relationship using a panel cellular biotest system including cell cultures for test objects. The cultures represented 4 differentiation types: normal human fibroblasts and myoblasts, human or Syrian hamster hepatoma cells, and mouse/mouse hybridoma cells. It was found that three widely used antibiotics gentamicin, kanamycin, and neomycin exhibit similar, but not identical cytotoxicity parameters and differ distinctly from geneticin.

Structural changes in abdominal aorta and vena cava inferior after experimental microwave destruction.

We studied morphological changes in rat abdominal aorta and vena cava inferior induced by endovascular microwave destruction. This treatment induced thrombosis and necrosis of the vascular walls, but fibrous framework was not completely destroyed. Then we observed irreversible changes in vessels: obliteration, atrophy of the aorta walls, and sclerosis of venous walls.