Separation of aromatic acids by wide-bore electrophoresis with nanoparticles prepared by electrospray as pseudostationary phase.

A model mixture of six aromatic acids has been separated using a laboratory-made wide-bore electrophoretic device with aminopropyl-modified nanoparticles used as pseudostationary phase. Optimization of preparation of nanoparticles by an electrospray (ES) method is described. With the optimized electrophoretic method, 30 mmol/L acetate running buffer, pH 4.

Determination of n-octanol-water partition coefficients by hollow-fiber membrane solvent microextraction coupled with HPLC.

A novel direct method has been developed for determination of n-octanol-water partition coefficients by hollow-fiber membrane solvent microextraction (HFMSME) combined with high-performance liquid chromatography (HPLC). The compound of interest is dissolved in water with sonication and a hollow fiber containing octanol inside is placed in the sample solution to perform microextraction. After microextraction the concentrations in both the aqueous and n-octanol phases are analyzed by HPLC with UV detection.