Influenza virus H1N1 induced apoptosis of mouse astrocytes and the effect on protein expression.

Objective: To investigate the effects of influenza A virus H1N1 infection on the proliferation and apoptosis of mouse astrocytes cells and its protein expression.

Methods: After mouse astrocytes was infected with purified influenza A virus H1N1 in vitro, viral integration and replication status of the cells were detected by RT-PCR assay, cell proliferation and apoptosis was determined by MTT method and flow cytometry, respectively. Associated protein expression was detected by Western blotting.

[Optical-fiber Fourier transform spectrometer].

A novel Fourier transform spectrum analyzer based on a single mode fiber Mach-Zehnder interferometer is reported. An optical fiber Fourier transform spectrometer, with bulk optics components replaced by fiber optical components and with the moving mirror replaced by a piezoelectric element fiber stretcher was constructed. The output spectrum of a LD below threshold was measured.

[Effects of simvastatin on homocysteine-induced endothelial dysfunction and inflammatory response].

Objective: To investigate the effects of simvastatin (SIM) on homocysteine (HCY)-induced endothelial dysfunction and inflammatory response.

Methods: Human umbilical vein endothelial cells (HUVECs) were isolated from the umbilical cords from healthy lying-in women and cultured and added with HCY of the concentrations of 0.1, 0.

Molecular modeling of the bacterial outer membrane receptor energizer, ExbBD/TonB, based on homology with the flagellar motor, MotAB.

The MotA/MotB proteins serve as the motor that drives bacterial flagellar rotation in response to the proton motive force (pmf). They have been shown to comprise a transmembrane proton pathway. The ExbB/ExbD/TonB protein complex serves to energize transport of iron siderophores and vitamin B12 across the outer membrane of the Gram-negative bacterial cell using the pmf.